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Study of Optical Properties of Macrophomina phaseolina Impregnated Sol-gel Derived Silica Matrices

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Abstract

In the present frame of work, Macrophomina phaseolina is encapsulated in silica matrices at various concentrations by low temperature sol-gel technique using tetraethylorthosilicate (TEOS) as precursor. The optical and photophysical properties of these samples have been studied by second harmonics of Nd:YAG laser at 532 nm. UV-visible absorption spectra of samples have been recorded and it is found that the absorption increases with increase in concentration of fungus. Further, a decrease in output transmission intensity of the laser has been observed with increase in fungus concentration. The temporal response of these samples has also been examined. The results show that the fungus concentration can be measured within ∼15–20 min. This method of optical sensing of fungus in test sample is faster than other techniques, such as the conventional colorimetric method which takes about 1 h.

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Acknowledgements

The financial support in the form of major research grant from Department of Science and Technology New Delhi and University Grant Commission New Delhi is gratefully acknowledged. Special thanks are given to Prof. Devendra Mohan for providing the laboratory facilities at his place to carry out this research work.

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Authors and Affiliations

  1. Department of Applied Physics, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India

    Sunita Sharma

  2. Department of Zoology, G.V.M. Girls College, Sonipat, Haryana, India

    Vandana

  3. Department of Physics, Faculty of Science, Addis Ababa University, P.O. Box-1176, Addis Ababa, Arat Kilo, Ethiopia

    S. K. Ghoshal

  4. Department of Bio and Nano-Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India

    Pooja Arora, Neeraj Dilbaghi & Ashok Chaudhury

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  1. Sunita Sharma
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  2. Vandana
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  3. S. K. Ghoshal
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  4. Pooja Arora
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  5. Neeraj Dilbaghi
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  6. Ashok Chaudhury
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Correspondence to Sunita Sharma.

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Sharma, S., Vandana, Ghoshal, S.K. et al. Study of Optical Properties of Macrophomina phaseolina Impregnated Sol-gel Derived Silica Matrices. Appl Biochem Biotechnol 159, 310–316 (2009). https://doi.org/10.1007/s12010-008-8323-z

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  • DOI: https://doi.org/10.1007/s12010-008-8323-z

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