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Production and Characterization of Cellulase-Free Xylanase from Trichoderma inhamatum

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Abstract

The production of extracellular cellulase-free xylanase from Trichoderma inhamatum was evaluated in liquid Vogel medium with different carbon sources as natural substrates and agricultural or agro-industrial wastes. Optimal production of 244.02 U/mL was obtained with xylan as carbon source, pH 6.0 at 25°C, 120 rpm, and 60-h time culture. Optimal conditions for enzyme activity were 50°C and pH 5.5. Thermal stability of T. inhamatum xylanolytic complex expressed as T1/2 was 2.2 h at 40°C and 2 min at 50°C. The pH stability was high from 4.0 to 11.0. These results indicate possible employment of such enzymatic complex in some industrial processes which require activity in acid pH, wide-ranging pH stability, and cellulase activity absence.

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Acknowledgement

We acknowledge Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq for the financial support and the scholarship awarded to the first author.

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  1. Biochemistry and Microbiology Department, Bioscience Institute, São Paulo State University, UNESP, Av: 24A, 1515, P.O. Box 199, CEP 13.506-900, Rio Claro, SP, Brazil

    Leonor Alves de Oliveira da Silva & Eleonora Cano Carmona

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  1. Leonor Alves de Oliveira da Silva
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  2. Eleonora Cano Carmona
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Correspondence to Eleonora Cano Carmona.

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de Oliveira da Silva, L.A., Carmona, E.C. Production and Characterization of Cellulase-Free Xylanase from Trichoderma inhamatum . Appl Biochem Biotechnol 150, 117–125 (2008). https://doi.org/10.1007/s12010-008-8296-y

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  • DOI: https://doi.org/10.1007/s12010-008-8296-y

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