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Breeding of d(–)-Lactic Acid High Producing Strain by Low-energy Ion Implantation and Preliminary Analysis of Related Metabolism

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Abstract

The low-energy nitrogen ion beam implantation technique was used in the breeding of mutant d(–)-lactic-acid-producing strains. The wild strain Sporolactobacillus sp. DX12 was mutated by an N+ ion beam with energy of 10keV and doses ranging from 0.4 × 1015 to 6.60 × 1015 ions/cm2. Combined with an efficient screening method, an efficient mutant Y2-8 was selected after two times N+ ion beam implantation. By using the mutant Y2-8, 121.6g/l of d-lactic acid was produced with the molar yields of 162.1% to the glucose. The yield of d-lactic acid by strain Y2-8 was 198.8% higher than the wild strain. Determination of anaerobic metabolism by Biolog MT2 was used to analyze the activities of the concerned enzymes in the lactic acid metabolic pathway. The results showed that the activities of the key enzymes responded on the substrates such as 6-phosphofructokinase, pyruvate kinase, and d-lactate dehydrogenase were considerably higher in the mutants than the wild strain. These might be affected by ion beam implantation.

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References

  1. Wee, Y. J., Kim, J. N., & Ryu, H. W. (2006). Food Technology and Biotechnology, 44, 163–172.

    CAS  Google Scholar 

  2. Ikada, Y., Jamshidi, K., Tsuji, H., & Hyon, S. H. (1987). Macromolecules, 20, 904–906.

    Article  CAS  Google Scholar 

  3. Tsuji, H., & Fukui, I. (2003). Polymer, 44, 2891–2896.

    Article  CAS  Google Scholar 

  4. Bustos, G., Moldes, A. B., Alonso, J. L., & Vázquez, M. (2004). Food Microbiology, 21, 143–148.

    Article  CAS  Google Scholar 

  5. Tanaka, T., Masahiro, H., Suguru, T., Kenji, S., Sadami, O., & Masayuki, T. (2006). Bioresource Technology, 97, 211–217.

    Article  CAS  Google Scholar 

  6. Ishida, N., Suzuki, T., Tokuhiro, K., Nagamori, E., Onishi, T., Saitoh, S., et al. (2006). Journal of Bioscience and Bioengineering, 101, 172–177.

    Article  CAS  Google Scholar 

  7. Yu, Z. L., Deng, J. G., He, J. J., Huo, Y. P., Wu, Y. J., Wang, X. D., et al. (1991). Nuclear Instruments & Methods in Physics Research, Section B, 59–60, 705–708.

    Article  Google Scholar 

  8. Yu, Z. L., Yang, J. B., Wu, Y. J., Cheng, B. J., He, J. J., & Huo, Y. P. (1993). Nuclear Instruments & Methods in Physics Research, Section B, 80–81, 1328–1331.

    Google Scholar 

  9. Chen, Y., Lin, Z. X., Zou, Z. Y., Zhang, F., Liu, D., Liu, X. H., et al. (1998). Nuclear Instruments & Methods in Physics Research, Section B, 140, 341–348.

    Article  CAS  Google Scholar 

  10. Gu, S. B., Yao, J. M., Yuan, Q. P., Xue, P. J., Zheng, Z. M., Wang, L., et al. (2006). Applied Microbiology and Biotechnology, 72, 456–461.

    Article  CAS  Google Scholar 

  11. Huang, W. D., Yu, Z. L., & Zhang, Y. H. (1998). Chemical Physics, 237, 223–231.

    Article  CAS  Google Scholar 

  12. Feng, H. Y., Yu, Z. L., & Chu, P. K. (2006). Materials Science & Engineering. R, 54, 49–120.

    Article  Google Scholar 

  13. Ding, Z. J., Bai, Z. Z., Sun, Z. H., Ouyang, P. K., & He, B. F. (2004). Chinese Journal of Bioprocess Engineering, 2, 30–36 (in Chinese).

    CAS  Google Scholar 

  14. Shigenobu, M., Lies, D., Tomohiro, A., Minako, H., Liu, T. J., & Mitsuyasu, O. (2004). Journal of Bioscience and Bioengineering, 97, 19–23.

    Google Scholar 

  15. Guckert, J. B., Carr, G. J., Johnson, T. D., Hamm, B. G., Davidson, D. H., & Kumagai, Y. (1996). Journal of Microbiological Methods, 27, 183–197.

    Article  Google Scholar 

  16. Covert, M. W., Knight, E. M., Reed, J. L., Herrgard, M. J., & Palsson, B. O. (2004). Nature, 429, 92–96.

    Article  CAS  Google Scholar 

  17. Hobbie, E. A., Watrud, L. S., Maggard, S. M., Shiroyama, T., & Rygiewicz, P. T. (2003). Soil Biology & Biochemistry, 35, 303–311.

    Article  CAS  Google Scholar 

  18. Koo, B. M., Yoon, M. J., Lee, C. R., Nam, T. W., Choe, Y. J., Jaffe, H., et al. (2004). The Journal of Biological Chemistry, 279, 31613–31621.

    Article  CAS  Google Scholar 

  19. Dos Santos, L. F., Defrenne, L., & Krebs-Brown, A. (2002). Analytica Chimica Acta, 456, 41–45.

    Article  Google Scholar 

  20. Su, C. X., Zhou, W., Fan, Y. H., Li, W., Zhao, S. G., & Yu, Z. L. (2006). Journal of Industrial Microbiology & Biotechnology, 33, 1037–1042.

    Article  CAS  Google Scholar 

  21. Okino, S., Suda, M., Fujikura, K., Inui, M., & Yukawa, H. (2008). Applied Microbiology and Biotechnology, 78, 449–454.

    Article  CAS  Google Scholar 

  22. Calabia, B. P., & Tokiwa, Y. (2007). Biotechnology Letters, 29, 1329–1332.

    Article  CAS  Google Scholar 

  23. De Vos, W. M., & Hugenholtz, J. (2004). Trends in Biotechnology, 22, 72–79.

    Article  Google Scholar 

  24. Wang, Q., Zhang, G., Du, Y. H., Zhao, Y., & Qiu, G. Y. (2003). Mutation Research, 528, 55–60.

    CAS  Google Scholar 

  25. Gu, S. B., Li, S. C., Feng, H. Y., Wu, Y., & Yu, Z. L. (2008). Applied Microbiology and Biotechnology, 78, 201–209.

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Long Yu of the Nanjing University of Technology for technical support. This work was supported by the 973 program (grant no. 2003CB716004) and the 863 program (grant no. 2006AA020102) of China.

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  1. College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Xinmofan Road 5, Nanjing, 210009, Jiangsu, China

    Ting-Ting Xu, Zhong-Zhong Bai, Li-Juan Wang & Bing-Fang He

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  1. Ting-Ting Xu
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  2. Zhong-Zhong Bai
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  3. Li-Juan Wang
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Correspondence to Bing-Fang He.

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Xu, TT., Bai, ZZ., Wang, LJ. et al. Breeding of d(–)-Lactic Acid High Producing Strain by Low-energy Ion Implantation and Preliminary Analysis of Related Metabolism. Appl Biochem Biotechnol 160, 314–321 (2010). https://doi.org/10.1007/s12010-008-8274-4

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