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Isolation and Identification of a Newly Isolated Alternaria sp. ND-16 and Characterization of Xylanase

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Abstract

Alternaria sp. ND-16, a bacterium isolated from soil sample, was identified as a strain of Alternaria mali based on the morphology and comparison of internal transcribed spacer rDNA gene sequence studies. Furthermore, it is demonstrated that this strain has xylanase activity, and the activity can be optimized under suitable growing conditions where wheat bran and urea are the primary sources of carbon and nitrogen. Partially purified xylanase from Alternaria sp. ND-16 is shown to have an optimal pH of 6.0 and optimal temperature of 50 °C, making this enzyme potentially suitable for industrial applications. It is also demonstrated that Na+ and Mn2+ show strong inhibition of the xylanase while K+, Li+, Fe2+, Cu2+, and Zn2+ have no significant effect on the activity.

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Acknowledgements

The authors greatly appreciate the help of Dr. Peter Baker of the Department of Chemical and Biological Science, Polytechnic University, USA, for his kindness in editing this manuscript. This work was supported by the Natural Science Foundation of Zhejiang Province (No. Y506136).

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Authors and Affiliations

  1. Department of Plant Science, North Dakota State University, Fargo, ND, 58105, USA

    Yin Li

  2. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Zhiqiang Liu

  3. NSF I/UCRC for Biocatalysis and Bioprocessing of Macromolecules, Polytechnic Institute of New York University, Six Metro Tech Center, Brooklyn, New York, 11201, USA

    Zhiqiang Liu & Geng Li

  4. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 2120013, People’s Republic of China

    Fengjie Cui

  5. Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Lifeng Ping

  6. School of Biotechnology, Chongqing Institute of Technology, Chongqing, 400050, People’s Republic of China

    Chongyan Qiu & Lijiao Yan

  7. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Lijiao Yan

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  1. Yin Li
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Correspondence to Yin Li, Zhiqiang Liu or Lijiao Yan.

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Li, Y., Liu, Z., Cui, F. et al. Isolation and Identification of a Newly Isolated Alternaria sp. ND-16 and Characterization of Xylanase. Appl Biochem Biotechnol 157, 36–49 (2009). https://doi.org/10.1007/s12010-008-8239-7

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  • DOI: https://doi.org/10.1007/s12010-008-8239-7

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