Abstract
We proposed and developed a series of fluorescent methods for analysis and investigation of biological systems with a view of future biotechnological and biomedical applications. The methods we describe have been built upon several photochemical and photophysical phenomena including fluorescent quenching, photochrome photoisomerization, and energy transfer. Three new types of molecular probes have been developed and employed for such studies: (1) dual fluorophore–nitroxide compounds, (2) fluorescence–photochrome molecules, and (3) super molecules containing both fluorescence and fluorescent quenching segments. The fluorescent properties of the new probes were intensively exploited for several practical applications including a real-time analysis of antioxidants, nitric oxide, superoxide, reactive radicals, trinitrotoluene, and metal ions, investigation of molecular dynamics of biomembranes in a wide range characteristic times, detection of protein conformational transition, and characterization of surface system. Owning high sensitivity, simplicity, and availability of fluorescent techniques, these methods can be widely employed and are adaptable to fibrooptic sensoring. A general survey of the physical principles and application of the new fluorescent methods has been provided.
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References
Likhtenstein, G. I., Ishii, K., & Nakatsuji, S. (2007). Photochemistry and Photobiology, 83, 871–881.
Papper, V., & Likhtenshtein, G. I. (2001). Journal of Photochemistry and Photobiology. A, Chemistry, 140, 39–52.
Nagii, V. Y., Bystryak, I. M., Kotel’nikov, A. I., Likhtenshtein, G. I., Petrukhin, O. M., Zolotov, Y. A. et al. (1990). Analyst (Cambridge, United Kingdom) 115(6), 839–841.
Likhtenshtein, G. I. (2005). Labeling, Biophysical. In R. Meyers (Ed.), Encyclopedia of Molecular Biology and Molecular Medicine (vol. Vol 7, (pp. 157–178)). New York: VCH.
Lozinsky, E., Shames, A., & Likhtenshtein, G. I. (2000). Dual fluorophore–nitroxides: Models for investigation of intramolecular quenching and novel redox probes. In S.G. Pandalai (Ed.), Recent research development in photochemistry and photobiology. Transworld Research Network (Vol. 2). India: Trivandrtum.
Likhtenshtein, G. I. (1993). Biophysical labeling methods in molecular biology. Cambridge, NY: Cambridge University Press.
Likhtenshtein, G. I. (2003). New Trends In Enzyme Catalysis and Mimicking Chemical Reactions. New York: Kluwer.
Chen O., Glaser R., & Likhtenshtein, G. I. (2008). Journal of Biochemical and Biophysical Methods (in press).
Likhtenshtein, G. I., Papper, V., Pines, D., Pines, E. (1997). Photochemical and photophysical characterization of 4,4¢- substituted stilbenes: linear free energy. In S. G. Pandalai (Ed.), Recent research development in photochemistry and photobiology (pp. 205–250). Transworld Research Network (Vol. 1). India: Trivandrtum.
Medvedeva, N., Martin, V. V., & Likhtenshten, G. I. (2004). Journal of Photochemistry and Photobiology. A, Chemistry, 163, 45–51.
Likhtenshtein, G. I., Bogatyrenko, V. R., Kulikov, A. V., Hideg, K., Hankovsy, G. O., Lukoyanov, N. V., et al. (1980). Doklady Akademii Nauk SSSR, 253(2), 481–484 [Biophys.].
Bystryak, I. M., Likhtenshtein, G. I., Kotel’nikov, A. I., Hankovsky, O., & Hideg, K. (1986). Russian Journal of Physical Chemistry, 60, 1679–1683.
Likhtenshtein, G. I., Yamauchi, J., Nakatuji, S., Smirnov, A., Tamura R. Nitroxides: Application in Chemistry, Biomedicine, and Materials Science (WILEY-VCH, in press)
Likhtenshtein, G. I., Febrario, F., & Nucci, R. (2000). Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy, 56, 2011–2031.
Vogel, V. R., Rubtsova, E. T., Likhtenshtein, G. I., & Hideg, K. (1994). Journal of Photochemistry and Photobiology. A, Chemistry, 83(3), 229–236.
Lozinsky, E., Martin, V. V., Berezina, T. A., Shames, A. I., Weis, A. L., & Likhtenshtein, G. I. (1999). Dual fluorophore–nitroxide probes for analysis of vitamin C in biological liquids. Journal of Biochemical and Biophysical Methods, 38(1), 29–42.
Lozinsky, E., Novoselsky, A., Shames, A. I., Saphier, O., Likhtenshtein, G. I., & Meyerstein, D. (2001). Biochimica et Biophysica Acta, General Subjects, 1526(1), 53–60.
Saphier, O., Silberstein, T., Shames, A. I., Likhtenshtein, G. I., Maimon, E., Mankuta, D., et al. (2003). Free Radical Research, 37, 301–308.
Packer, L. (1994). Nitric oxide. In Methods in Enzymology (pp. 739–749). San Diego: Academic.
Stuehr, D. J., & Ghosh, S. (2000). Enzymology of nitric oxide synthases. Handbook of Experimental Pharmacology, 143, 33–70 (Nitric Oxide).
Lozinsky, E. M., Martina, L. V., Shames, A. I., Uzlaner, N., Masarwa, A., Likhtenshtein, G. I., et al. (2004). Analytical Biochemistry, 326(2), 139–145.
Blough, N. V., & Simpson, D. J. (1988). Journal of the American Chemical Society, 110, 1915–1917.
Pou, S., Huang, Y. I., Bhan, A., Bhadti, V. S., Hosmane, R. S., Wu, S. Y., et al. (1993). Analytical Biochemistry, 212, 85–90.
Borisenko, G., Martin, I., Zhao, Q., & Kagan, V. E. (2004). Journal of the American Chemical Society, 126, 9221–9232.
Bian, Z.-Y., Guo, X.-Q., Zhao, Y.-B., & Du, J.-O. (2005). Analytical Sciences, 21, 553–559.
Coenjarts, C., García, O., Llauger, L., Palfreyman, J., Vinette, A. L., & Scaiano, J. C. (2003). Journal of the American Chemical Society, 125, 620–621.
Sun, Y. P., & Saltiel, J. (1989). Journal of Physical Chemistry, 93, 8310–8316.
Likhtenshtein, G. I., Papper, V., Pines, D., Pines, E. (1997). Photochemical and photophysical characterization of 4,4¢-substituted stilbenes: linear free energy. In S. G. Pandalai (Ed.), Recent research development in photochemistry and photobiology (pp. 205–250). Transworld Research Network (Vol. 1). India: Trivandrtum.
Papper, V., Likhtenshtein, G. I., Pines, D., & Pines, E. (1997). Journal of Photochemistry and Photobiology. A, Chemistry, 111, 87–96.
Mekler, V. M., & Likhtenshtein, G. I. (1986). Biofizika, 31, 568–571.
Likhtenshtein, G. I., Bishara, R., Papper, V., Uzan, B., Fishov, I., Gill, D., et al. (1996). Journal of Biochemical and Biophysical Methods, 33(2), 117–133.
Papper, V., Likhtenshtein, G. I., Medvedeva, N., & Khoudyakov, D. V. (1999). Journal of Photochemistry and Photobiology. A, Chemistry, 122, 79–85.
Papper, V., Medvedeva, N., Fishov, I., & Likhtenshtein, G. I. (2000). Applied Biochemistry and Biotechnology, 89(2–3), 231–248.
Medvedeva, N., Papper, V., & Likhtenshten, G. I. (2005). Physical Chemistry Chemical Physics, 7, 3368–3374.
Mekler, V. M., & Umarova, F. T. (1988). Biofizika, 33, 720–722.
Likhtenshtein, G. I., Khudjakov, D. V., & Vogel, V. R. (1992). Journal of Biochemical and Biophysical Methods, 25(4), 219–229.
Chen, O., Glaser, R., & Likhtenshtein, G. I. (2003). Biophysical Chemistry, 103, 139–156.
Ahluwalia, A., Papper, V., Chen, O., Likhtenshtein, G. I., & De Rossi, D. (2002). Analitical Biochemistry, 305, 121–134.
Razi Naqvi, K., Martins, J., & Melo, E. (2000). Phys. Chem. B., 104, 12035–12038.
Chen, O., Uzlaner, N., Priel, Z., Likhtenshtein, G. I. (2008). Journal of Biochemical and Biophysical Methods (accepted).
Parkhomyuk-Ben Arye, P. (2004). Development of multifunctional fluorescent probes for analysis of nitric oxide and antioxidants. Doctor of Phylosophy Tesis, Ben-Gurion University of the Negev, Beer-Sheva, Israel (in English).
Strashnikova, N. V., Papper, V., Parhomyuk, P., Ratner, V., Likhtenshtein, G. I., & Marks, R. (1999). Journal of Photochemistry and Photobiology. A, Chemistry, 122, 133–142.
Parkhomyuk-Ben Arye, P., Strashnikova, N., & Likhtenshtein, G. I. (2002). Journal of Biochemical and Biophysical Methods, 51, 1–15.
Strashnikova, N. V., Medvedeva, N., & Likhtenshtein, G. I. (2001). Journal of Biochemical and Biophysical Methods, 48, 43–60.
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Likhtenshtein, G.I. Novel Fluorescent Methods for Biotechnological and Biomedical Sensoring: Assessing Antioxidants, Reactive Radicals, NO Dynamics, Immunoassay, and Biomembranes Fluidity. Appl Biochem Biotechnol 152, 135–155 (2009). https://doi.org/10.1007/s12010-008-8219-y
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DOI: https://doi.org/10.1007/s12010-008-8219-y