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Expression and Production of Human Interleukin-7 in Insect Cells Using Baculovirus Expression Vector System (BEVS)

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Abstract

Interleukin-7 (IL-7) is a glycoprotein cytokine with significant clinical and biomedical potential, such as cancer therapy and HIV infections. Earlier it has been cloned and expressed in various protein expression systems; however, they are not efficient for large-scale production. To address this inadequacy, we report in this paper the production of recombinant human interleukin-7 (hIL-7) in insect cells. A recombinant bacmid containing hIL-7 was constructed, purified, and characterized. It was used to infect Trichoplusia ni (BT1-TN-5B1/High Five™) insect cells. Result shows that T. ni cells successfully produce hIL-7 in shake flask cultures. A scale up to 2.5-L laboratory batch bioreactor showed the efficacy of this system for large-scale production. Our results offer a highly efficient, inexpensive, and convenient system for the large-scale expression and production of recombinant hIL-7.

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Acknowledgements

This work was supported by research grants (to S.P.) from the Canadian Institute of Health Research (CIHR). The authors acknowledge Dr. Yan Xu for her advice in the plasmid construction, Johnny Montes for the technical assistance and Jasmine Rohinton Bhathena for manuscript proof reading. M. M. acknowledges a Ph.D. Scholarship from the Iranian Ministry of Health and Education.

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Authors and Affiliations

  1. Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada

    Maryam Mirzaei & Satya Prakash

  2. Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada

    Maryam Mirzaei & Satya Prakash

  3. Biotechnology Research Institute (BRI), National Research Council (NRC), 6100 Royalmount Avenue, Montreal, QC, H4P 2R2, Canada

    Maryam Mirzaei, Barbara Jardin & Cynthia B. Elias

Authors
  1. Maryam Mirzaei
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  2. Barbara Jardin
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  3. Cynthia B. Elias
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  4. Satya Prakash
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Correspondence to Satya Prakash.

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Mirzaei, M., Jardin, B., Elias, C.B. et al. Expression and Production of Human Interleukin-7 in Insect Cells Using Baculovirus Expression Vector System (BEVS). Appl Biochem Biotechnol 151, 93–103 (2008). https://doi.org/10.1007/s12010-008-8178-3

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  • DOI: https://doi.org/10.1007/s12010-008-8178-3

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