Skip to main content
SpringerLink
Log in

Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The lipase inhibitory activity of ethanol extracts obtained from some marine algae collected on the Tunisian coast was evaluated. Caulerpa prolifera extract markedly reduced both dog gastric (DGL) and human pancreatic lipase (HPL) activities. Generally, the inhibition reached 100% after 40 to 60 min of incubation depending on lipase types and on substrates used. Moreover, the inhibitory effect of C. prolifera extract on lipases appeared to be accelerated by adding bile salts, which likely modified the interface and allowed the inhibitory compound to inactivate the lipase. The separation of C. prolifera extract by thin-layer chromatography (TLC) resulted in eight fractions showing efficient inhibition rate against DGL, compared to the crude extract. In the case of HPL, TLC fractionation reduced the inhibitory rates, suggesting that the effect of algal extract on lipases may be caused by a synergetic action of several compounds within the extract. High-performance liquid chromatograph separation resulted in isolation of a major compound displaying high inhibition capacity of HPL activity. Caulerpa prolifera extract may therefore be useful in developing antiobesity drugs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Kopelman, P. G. (2000). Nature, 404, 635–643.

    CAS  Google Scholar 

  2. Gallup, E., & Dujovne, C. (2001). Expert Opinion on Investigational Drugs, 10, 561–567.

    Article  CAS  Google Scholar 

  3. Yanovski, S. Z., & Yanovski, J. A. (2002). New England Journal of Medicine, 346, 591–602.

    Article  CAS  Google Scholar 

  4. Bernback, S., Blackberg, L., & Hernell, O. (1990). Journal of Clinical Investigation, 85, 1221–1226.

    Article  CAS  Google Scholar 

  5. Borel, P., Armand, M., Sentft, M., Lafont, H., & Lairon, D. (1991). Gastroenterology, 100, 1582–1589.

    CAS  Google Scholar 

  6. Carey, M. C., Small, D. M., & Bliss, C. M. (1983). Annual Review of Physiology, 45, 651–677.

    Article  CAS  Google Scholar 

  7. Hadavary, P., Lengsfeld, H., & Wolfer, H. (1988). Biochemical Journal, 256, 357–361.

    Google Scholar 

  8. Hauptman, J. B., Jeunt, F. S., & Hartmann, D. (1992). American Journal of Clinical Nutrition, 55, 309–313.

    Google Scholar 

  9. Drent, M. L., Larsson, I., William-Olsson, T., Quaade, F., Czubayko, F., Von Bergmann, K., et al. (1995). International Journal of Obesity, 19, 221–226.

    CAS  Google Scholar 

  10. Sjostrom, L., Rissanen, A., Andersen, T., Boldrin, M., Golay, A., Koppeschaar, H. P. F., et al. (1998). Lancet, 352, 167–172.

    Article  CAS  Google Scholar 

  11. Wang, S. M., & Huang, A. H. C. (1984). Plant Physiology, 76, 929–934.

    Article  CAS  Google Scholar 

  12. Gargouri, Y., Julien, R., Pieroni, G., Verger, R., & Sarda, L. (1984). Journal of Lipid Research, 25, 1214–1221.

    CAS  Google Scholar 

  13. Juhel, C., Armand, M., Pafumi, Y., Rosier, C., Vandermander, J., & Lairon, D. (2000). Journal of Nutritional Biochemistry, 11, 45–51.

    Article  CAS  Google Scholar 

  14. Gargouri, Y., Julien, R., Sugihara, A., Verger, R., & Sarda, L. (1984). Biochimica et Biophysica Acta, 795, 326–331.

    CAS  Google Scholar 

  15. Brockerhoff, H. (1974). In H. Brockerhoff, & R. G. Jensen (Eds.) Lipolytic enzymes pp. 25–125. New York: Academic.

    Google Scholar 

  16. Sharma, N., Sharma, V. K., & Seo, S. Y. (2005). Journal of Ethnopharmacology, 97, 453–456.

    Article  Google Scholar 

  17. Shimura, S., Tsuzuki, W., Kobayashi, S., & Suzuki, T. (1992). Bioscience, Biotechnology, and Biochemistry, 56, 1478–1479.

    Article  CAS  Google Scholar 

  18. Gargouri, Y., Chahinian, H., Moreau, H., Ransac, S., & Verger, R. (1991). Biochimica et Biophysica Acta, 1085, 322–328.

    CAS  Google Scholar 

  19. Gargouri, Y., Cudrey, C., Mejboub, H., & Verger, R. (1992). European Journal of Biochemistry, 204, 1063–1067.

    Article  CAS  Google Scholar 

  20. Gargouri, Y., Pieroni, G., Riviere, C., Sauniere, J. F., Lowe, P. A., Sarda, L., et al. (1986). Gastroenterology, 91, 919–925.

    CAS  Google Scholar 

  21. Tsujita, T., Sumiyoshi, M., Takaku, T., Momsen, W. E., Lowe, M. E., & Brockman, H. L. (2003). Journal of Lipid Research, 44, 2278–2286.

    Article  CAS  Google Scholar 

  22. Gargouri, Y., Pieroni, G., Lowe, P. A., Sarda, L., & Verger, R. (1986). European Journal of Biochemistry, 156, 305–310.

    Article  CAS  Google Scholar 

  23. Bitou, N., Ninomiya, M., Tsujita, T., & Okuda, H. (1999). Lipids, 34, 441–445.

    Article  CAS  Google Scholar 

  24. Barwell, C. J., Blunden, G., & Manandhar, D. (1989). Journal of Applied Phycology, 1, 319–323.

    Article  Google Scholar 

  25. Maréchal, J. P., Culioli, G., Hellio, C., Thomas-Guyon, H., Callow, M. E., Clare, A. S., et al. (2004). Journal of Experimental Marine Biology and Ecology, 313, 47–62.

    Article  Google Scholar 

  26. Pavia, H., & Toth, G. B. (2000). Hydrobiologia, 440, 299–305.

    Article  CAS  Google Scholar 

  27. Steinberg, P. D., & Van Altena, I. (1992). Ecological Monographs, 62, 189–222.

    Article  Google Scholar 

  28. Ardevol, A., Blade, C., Salvado, M. J., & Arola, L. (2000). International Journal of Obesity, 24, 319–324.

    Article  CAS  Google Scholar 

  29. Moreno, D. A., Ilic, N., Poulev, A., Brasaemle, D. L., Fried, S. K., & Raskin, I. (2003). Nutrition, 19, 876–879.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut National des Sciences et Technologies de la Mer-Sfax B.P. 1035, Sfax, 3018, Tunisia

    Faouzi Ben Rebah

  2. Laboratoire de biochimie et de génie enzymatique des lipases, ENIS, BPW, 3038, Sfax, Tunisia

    Sana Smaoui, Fakher Frikha, Youssef Gargouri & Nabil Miled

Authors
  1. Faouzi Ben Rebah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sana Smaoui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fakher Frikha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Youssef Gargouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nabil Miled
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Faouzi Ben Rebah.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ben Rebah, F., Smaoui, S., Frikha, F. et al. Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases. Appl Biochem Biotechnol 151, 71–79 (2008). https://doi.org/10.1007/s12010-008-8167-6

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-008-8167-6

Keywords

Search

Navigation