Abstract
The growing interest in natural food has raised the global demand for nutraceuticals. We studied enhanced production of biomass, delta-aminolevulinic acid (δ-ALA), bili pigments and antioxidant capacity of a food alga Nostochopsis lobatus in a full-factorial (three level) design with supplemental Zn, glutamine, and Zn + glutamine in batch culture. Production of biomass, pigments, and antioxidant capacity all were higher under immobilized cell cultures in comparison to free cell cultures. Maximum biomass (2,390 mg dry wt l−1), δ-ALA (2.715 μg mg−1 dry wt h−1), phycocyanin (98.50 mg g−1 dry wt), phycoerythrin (158.0 mg g−1 dry wt), and antioxidant capacity (140.50 μmoles ascorbic acid equivalent capacity g−1 fresh wt) were recorded when Zn and glutamine were supplemented together in the growth medium at pH 7.8. These effects were found to be significantly related to the activities of glutamine synthetase (GSmax: 490.2 nmoles mg protein−1 min−1), glutamate synthase (GOGATmax: 27.0 nmoles mg protein−1 min−1), and glutamate dehydrogenase (GDHmax: 159.9 nmoles mg protein−1 min−1). This study shows that N. lobatus could be a promising bioresource for the production of nutritionally rich biomass, δ-ALA, bili pigments, and antioxidants. Use of immobilized cells in batch culture supplemented with Zn and glutamine could be an effective approach for scaling up production for commercial use.
Similar content being viewed by others
References
Kumar, K., Lakshmanan, A., & Kannaiyan, S. (2003). Indian Journal of Microbiology, 43, 9–16.
Thajuddin, N., & Subramaniam, G. (2005). Current Science, 29, 47–57.
Parikh, A., & Madamwar, D. (2006). Bioresource Technology, 97, 1822–1827.
Colla, L. M., Reinehr, C. O., Reichert, C., & Costa, J. A. V. (2007). Bioresource Technology, 98, 1489–1493.
Boyde, M. R. (1997). Antimicrobial Agents and Chemotherapy, 41, 1521–1530.
Pandey, U. (2003). Indian Journal of Applied and Pure Biology, 18, 27–30.
Sies, H. (1996). Antioxidants in disease, mechanisms and therapy. New York: Academic Press.
Jose, N., & Janardhanan, K. K. (2000). Current Science, 79, 941–943.
Devasagayan, T. P. A., Tilak, J. C., Boloor, K. K., Sane, K. K., Ghaskadbi, S., & Lele, R. D. (2004). Journal of Association of Physicians of India, 7, 794–804.
Mishra, A., Bapat, M. M., Tilak, J. C., & Devasagayam, T. P. A. (2006). Current Science, 91, 90–93.
Hirata, T., Tanak, M., Ooike, M., Tsunomura, T., & Sakaguchi, M. (2000). Journal of Applied Phycology, 12, 435–439.
Romay, C., Gonzalez, R., Ledon, N., Remirez, D., & Rimbau, V. (2003). Current Protein and Peptide Science, 4, 207–216.
Döring, F., Walter, J., Will, J., Föcking, M., Boll, M., Amasheh, S., et al. (1998). Journal of Clinical Investigation, 101, 2761–2767.
Inskeep, W. P., & Bloom, P. R. (1985). Plant Physiology, 77, 483–485.
Bennett, A., & Bogorad, L. (1973). Journal of Cell Biology, 58, 419–435.
Hirosawa, T., & Miyachi, S. (1983). Plant Science Letters, 28, 291–298.
Shapiro, B. M., & Stadtman, E. R. (1970). In H. Tabor, & C. W. Tabor (Eds.) Methods in enzymology pp. 910–922. New York: Academic Press.
Thevanathan, R. (1980). Ph.D. Thesis, University of Madras, India.
Ahmed, I., & Hellebust, J. A. (1984). Plant Physiology, 76, 658–663.
Cao, G., & Prior, R. L. (1999). Methods in Enzymology, 299, 50–62.
Benzie, I. F., & Strain, J. J. (1996). Analytical Biochemistry, 239, 70–76.
Mahajan, G., & Kamat, M. (1995). Applied Microbiology and Biotechnology, 43, 466–469.
Dillion, J. C., & Phuc, A. P. (1995). World Review of Nutrition and Dietetics, 77, 32–46.
Emodi, A. (1978). Food Technology, 32, 38–42.
Rodriguez, H., Rivas, J., Guerrero, M. G., & Losada, M. (1989). Applied and Environmental Microbiology, 55, 758–760.
O’ Neill, G. P. O., Peterson, D. M., Schön, A., Chen, M. W., & Söll, D. (1988). Journal of Bacteriology, 170, 3810–3816.
Bannister, J. V., Bannister, W. H., & Rotilio, G. (1987). Critical Reviews in Biochemistry, 22, 111–180.
Herrero, M., Martin-Alvarez, P. J., Senorans, J., Cifuentes, A., & Ibanez, E. (2005). Food Chemistry, 93, 417–423.
Tilak, J. C., Banerjee, M., Mohan, H., & Devasagayam, T. P. A. (2004). Phytotheraphy Research, 18, 798–804.
Cao, G., Sofic, E., & Prior, R. L. (1996). Journal of Agricultural and Food Chemistry, 44, 3426–3431.
Powel, S. R. (2000). Journal of Nutrition, 130, 1447S–1454S.
Zago, M. P., & Oteiza, P. I. (2001). Free Radical Biology & Medicine, 31, 266–274.
Acknowledgement
The authors are thankful to Prof. H. R. Tyagi, former Convener, Department of Environmental Sciences, MLS University, for laboratory facilities.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pandey, U., Pandey, J. Enhanced Production of High-Quality Biomass, δ-Aminolevulinic Acid, Bilipigments, and Antioxidant Capacity of a Food Alga Nostochopsis lobatus . Appl Biochem Biotechnol 150, 221–231 (2008). https://doi.org/10.1007/s12010-008-8149-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8149-8