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Effect of Agitation and Aeration Rates on Chitinase Production Using Trichoderma virens UKM1 in 2-l Stirred Tank Reactor

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Abstract

Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-d-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the k L a of 8.34 per hour.

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Acknowledgment

The authors acknowledged the financial support from the Ministry of Science, Technology and Innovation, Malaysia (MOSTI).

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Authors and Affiliations

  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Suraini Abd-Aziz & Mohd. Ali Hassan

  2. Laboratory of Industrial Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Suraini Abd-Aziz & Christine Cheryl Fernandez

  3. Department of Biology, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Madihah Md. Salleh

  4. Department of Bioprocess Engineering, Faculty of Chemical Engineering and Natural Resources Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Rosli Md. Illias

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  1. Suraini Abd-Aziz
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  4. Rosli Md. Illias
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Correspondence to Suraini Abd-Aziz.

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Abd-Aziz, S., Fernandez, C.C., Salleh, M.M. et al. Effect of Agitation and Aeration Rates on Chitinase Production Using Trichoderma virens UKM1 in 2-l Stirred Tank Reactor. Appl Biochem Biotechnol 150, 193–204 (2008). https://doi.org/10.1007/s12010-008-8140-4

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  • DOI: https://doi.org/10.1007/s12010-008-8140-4

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