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Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems

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Abstract

In this study, a novel pH-sensitive and reversible water-soluble polymer(PABC) forming aqueous two-phase systems(ATPS) was synthesized by using 2-(dimethylamino)ethyl methacrylate, t-butyl methacrylate, and methyl methacrylate as monomers and 2,2′-azo-bis-isobutyronitrile as initiator. The PABC could be recovered by adjusting isoelectric point (PI) to 8.4, and recovery at PI could reach 95%. ATPS was formed by 5% (w/w) PABC and 10% (w/w) PEG20000. The partition coefficient K of lysozyme was 6.8, and the partition coefficient K of bovine serum albumin could reach 12.5 in the ATPS.

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Acknowledgment

This project was supported by the Natural Scientific Foundation of China (20474016) and Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars.

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Authors and Affiliations

  1. State Key laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China

    Wei QIN & Xue-Jun CAO

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  1. Wei QIN
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  2. Xue-Jun CAO
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Correspondence to Xue-Jun CAO.

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QIN, W., CAO, XJ. Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems. Appl Biochem Biotechnol 150, 171–183 (2008). https://doi.org/10.1007/s12010-008-8139-x

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  • DOI: https://doi.org/10.1007/s12010-008-8139-x

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