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Monoglycerides and Diglycerides Synthesis in a Solvent-Free System by Lipase-Catalyzed Glycerolysis

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Abstract

Five lipases were screened (Thermomyces lanuginosus free and immobilized forms, Candida antarctica B, Candida rugosa, Aspergillus niger, and Rhizomucor miehei) to study their ability to produce monoglycerides (MG) and diglycerides (DG) through enzymatic glycerolysis of soybean oil. Lipase from C. antarctica was further studied to verify the enzyme load (wt% of oil mass), the molar ratio glycerol/oil, and the water content (wt% of glycerol) on the glycerolysis reaction. The best DG and MG productions were in the range 45–48% and 28–30% (w/w, based on the total oil), respectively. Using immobilized lipases, the amount of free fatty acids (FFA) produced was about 5%. However, the amount of FFA produced when using free lipases, with 3.5% extra water in the system, is equivalent to the MG yield, about 23%. The extra water content provides a competition between hydrolysis and glycerolysis reactions, increasing the FFA production.

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Acknowledgments

The authors are grateful for the financial support of CNPq and FAPESP.

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Authors and Affiliations

  1. Separation Process Development Laboratory, Chemical Engineering School, State University of Campinas, 13081 970, Campinas, São Paolo, Brazil

    Patricia Bogalhos Lucente Fregolente, Leonardo Vasconcelos Fregolente, Gláucia Maria F. Pinto, Benedito César Batistella, Maria Regina Wolf-Maciel & Rubens Maciel Filho

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  1. Patricia Bogalhos Lucente Fregolente
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  2. Leonardo Vasconcelos Fregolente
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  3. Gláucia Maria F. Pinto
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  4. Benedito César Batistella
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  5. Maria Regina Wolf-Maciel
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  6. Rubens Maciel Filho
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Correspondence to Patricia Bogalhos Lucente Fregolente.

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Fregolente, P.B.L., Fregolente, L.V., Pinto, G.M.F. et al. Monoglycerides and Diglycerides Synthesis in a Solvent-Free System by Lipase-Catalyzed Glycerolysis. Appl Biochem Biotechnol 146, 165–172 (2008). https://doi.org/10.1007/s12010-008-8133-3

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  • DOI: https://doi.org/10.1007/s12010-008-8133-3

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