Abstract
This article aims at the evaluation of the catalytic performance of glucose oxidase (GO) (EC.1.1.3.4) for the glucose/gluconic acid conversion in the ultrafiltration cell type membrane reactor (MB-CSTR). The reactor was coupled with a Millipore ultrafiltration-membrane (cutoff of 100 kDa) and operated for 24 h under agitation of 100 rpm, pH 5.5, and 30°C. The experimental conditions varied were the glucose concentration (2.5, 5.0, 10.0, 20.0, and 40.0 mM), the feeding rate (0.5, 1.0, 3.0, and 6.0/h), dissolved oxygen (8.0 and 16.0 mg/L), GO concentration (2.5, 5.0, 10.0, and 20.0 UGO/mL), and the glucose oxidase/catalase activity ratio (UGO/UCAT)(1:0, 1:10, 1:20, and 1:30). A conversion yield of 80% and specific reaction rate of 40 × 10−4 mmol/h·UGO were attained when the process was carried out under the following conditions: D =3.0/h, dissolved oxygen =16.0 mg/L, [G] =40 mM, and (UGO/UCAT) = 1:20. A simplified model for explaining the inhibition of GO activity by hydrogen peroxide, formed during the glucose/gluconic acid conversion, was presented.
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das Neves, L.C.M., Vitolo, M. Use of glucose oxidase in a membrane reactor for gluconic acid production. Appl Biochem Biotechnol 137, 161–170 (2007). https://doi.org/10.1007/s12010-007-9048-0
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DOI: https://doi.org/10.1007/s12010-007-9048-0