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Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase

  • Session 1A: Enzyme Catalysis And Engineering
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Abstract

The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50°C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.

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Authors and Affiliations

  1. Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, PHLC, sl. 427, CEP: 20559-900, RJ, RJ, Brazil

    Otávio L. Bernardes & Marta A. P. Langone

  2. Centro de Pesquisa e Desenvolvimento da Petrobras—Cenpes, Brazil

    Juliana V. Bevilaqua

  3. Instituto de Química, Universidade Federal do Rio de Janeiro, RJ, CEP: 21949-900, Brazil

    Márcia C. M. R. Leal & Denise M. G. Freire

Authors
  1. Otávio L. Bernardes
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  2. Juliana V. Bevilaqua
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  3. Márcia C. M. R. Leal
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  4. Denise M. G. Freire
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  5. Marta A. P. Langone
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Correspondence to Marta A. P. Langone.

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Bernardes, O.L., Bevilaqua, J.V., Leal, M.C.M.R. et al. Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase. Appl Biochem Biotechnol 137, 105–114 (2007). https://doi.org/10.1007/s12010-007-9043-5

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  • DOI: https://doi.org/10.1007/s12010-007-9043-5

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