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Spectroscopic Characterization of Thiazole Orange-3 DNA Interaction

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Abstract

The interaction of a new derivative of thiazole orange (TO-3) with calf thymus DNA (ctDNA) has been investigated by fluorescence and absorption spectroscopy. When TO-3 binds to ctDNA, absorption bands exhibit significant hypochromicity at low base pair/dye ratio (BP/D ratio), and high BP/D show hyperchromicity with red shift. The spectral changes are attributed to the different species formed between TO-3 and ctDNA in the titration course of the dye molecule with DNA. Multivariate curve resolutions–alternating least squares (MCR–ALS) is applied to the absorption measurements recorded to recover the concentration profiles and the pure spectra of the DNA/TO-3 complexes involved in the process. The binding constant and size of the binding site have been determined spectrophotometrically using the McGhee von Hippel equation. MCR–ALS has been used to reveal the precise concentration profiles of all detectable species formed between ctDNA and TO-3 and their pure spectral profiles.

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Authors and Affiliations

  1. Department of Chemistry, Razi University, Kermanshah, Iran

    J. Ghasemi & Sh. Ahmadi

  2. Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    A. I. Ahmad

  3. Department of Biology, Razi University, Kermanshah, Iran

    S. Ghobadi

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  1. J. Ghasemi
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  2. Sh. Ahmadi
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  3. A. I. Ahmad
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  4. S. Ghobadi
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Correspondence to J. Ghasemi.

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Ghasemi, J., Ahmadi, S., Ahmad, A.I. et al. Spectroscopic Characterization of Thiazole Orange-3 DNA Interaction. Appl Biochem Biotechnol 149, 9–22 (2008). https://doi.org/10.1007/s12010-007-8124-9

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