Applied Biochemistry and Biotechnology

, Volume 149, Issue 1, pp 89–98 | Cite as

A Simple Substrate Feeding Strategy using a pH Control Trigger in Fed-Batch Fermentation

  • Tiong-Ee Ting
  • Gregory J. ThomaEmail author
  • Robert R. BeitleJr.
  • Ralph K. Davis
  • Rugkiat Perkins
  • Khursheed Karim
  • Hui-Min Liu


A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture’s growth kinetics. To demonstrate the effectiveness of the coupled pH and glucose control strategy in biomass and/or secondary metabolite production, several fed-batch fermentations of indigenous Escherichia coli and recombinant E. coli were carried out. Both strains produced biomass with optical density of greater than 40 at 600 nm. We also tested the glucose control strategy using two types of pH controller: a less sophisticated portable pH controller and a more sophisticated online proportional-integral-derivative (PID) controller. Our control strategy was successfully applied with both controllers, although better control was observed using the PID controller. We have successfully demonstrated that a glucose feeding strategy based on a simple pH control scheme to indirectly control the glucose concentration can be easily achieved and adapted to conventional bioreactors in the absence of online glucose measurement and control.


E. coli Fed-batch Glucose regulation pH control 



This work was supported in part by a grant (EAR-0207793) from the National Science Foundation.


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Tiong-Ee Ting
    • 1
  • Gregory J. Thoma
    • 1
    Email author
  • Robert R. BeitleJr.
    • 1
  • Ralph K. Davis
    • 2
  • Rugkiat Perkins
    • 1
  • Khursheed Karim
    • 1
  • Hui-Min Liu
    • 3
  1. 1.Ralph E. Martin Department of Chemical EngineeringUniversity of ArkansasFayettevilleUSA
  2. 2.Department of GeosciencesUniversity of ArkansasFayettevilleUSA
  3. 3.Department of Biological SciencesUniversity of ArkansasFayettevilleUSA

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