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Substrate Dependency and Effect of Xylanase Supplementation on Enzymatic Hydrolysis of Ammonia-Treated Biomass

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Abstract

Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the important features of ammonia-based pretreatment is that most of the hemicellulose is retained after treatment, which simplifies the overall bioconversion process and enhances the conversion efficiency. The pretreatment processes were optimized for these feedstocks, taking carbohydrate retention as well as sugar yield in consideration. The data indicate that hybrid poplar is more difficult to treat than corn stover, thus, requires more severe conditions. On the other hand, hybrid poplar has a beneficial property that it retains most of the hemicellulose after pretreatment. To enhance the digestibility of ammonia-treated poplars, xylanase was supplemented during enzymatic hydrolysis. Because of high retention of hemicellulose in treated hybrid poplar, xylanase supplementation significantly improved xylan as well as glucan digestibility. Of the three feedstocks, best results and highest improvement by xylanase addition was observed with LL hybrid poplar, showing 90% of overall sugar yield.

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Acknowledgment

The authors acknowledge the financial support for this research from the US Department of Energy (financial assistance no. DE-PS36–00GO10482, channeled through Dartmouth College). They also would like to thank Genencor International (Paulo Alto, CA, USA) for providing enzymes used in this research and NREL for providing the feedstocks.

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Authors and Affiliations

  1. Department of Chemical Engineering, Auburn University, 207 Ross Hall, Auburn, AL, 36830, USA

    Rajesh Gupta & Yoon Y. Lee

  2. Eastern Regional Research Center NAA, ARS, USDA, 600 East Mermaid Lane, Wyndmoor, PA, 19038, USA

    Tae Hyun Kim

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  1. Rajesh Gupta
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  2. Tae Hyun Kim
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  3. Yoon Y. Lee
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Correspondence to Yoon Y. Lee.

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Gupta, R., Kim, T.H. & Lee, Y.Y. Substrate Dependency and Effect of Xylanase Supplementation on Enzymatic Hydrolysis of Ammonia-Treated Biomass. Appl Biochem Biotechnol 148, 59–70 (2008). https://doi.org/10.1007/s12010-007-8071-5

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  • DOI: https://doi.org/10.1007/s12010-007-8071-5

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