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Studies on Productivity and Characterization of Polygalacturonase from Aspergillus giganteus Submerged Culture Using Citrus Pectin and Orange Waste

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Abstract

Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natural substrates, were tested in standing cultures, and the best results were obtained with orange bagasse and purified citrus pectin. On citrus pectin as sole carbon source, the highest extracellular activity (9.5 U/ml and 40.6 U/mg protein) was obtained in 4.5-day-old cultures shaken at 120 rpm, pH 3.5 and 30°C, while on orange bagasse, the highest extracellular activity (48.5 U/ml and 78.3 U/mg protein) was obtained in 3.5-day-old cultures shaken at 120 rpm, pH 6.0 and 30°C. Optimal polygalacturonase activity was observed in assays conducted at pH 5.5–6.5 and 55–60°C. The activity showed good thermal stability, with half-lives of 90 and 30 min when incubated at 55 and 60°C, respectively. High stability was observed from pH 4.5 to 8.5; more than 90% of the activity remained after 24 h in this pH range.

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Acknowledgements

The authors are grateful to Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) for financial support and to CPKelco - Limeira, SP, Brazil, for their donation of citrus pectin.

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Authors and Affiliations

  1. Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista - Unesp., Av. 24A, 1515, CEP 13506-900, Rio Claro, SP, Brazil

    Danielle Biscaro Pedrolli & Eleonora Cano Carmona

  2. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista - Unesp., R. Cristóvão Colombo, 2265, CEP 15054-000, São José do Rio Preto, SP, Brazil

    Eleni Gomes

  3. Departamento de Alimentos e Nutrição, Faculdade de Ciências Farmaceuticas, Universidade Estadual Paulista - Unesp., Rodovia Araraquara/Jau Km 1, CEP 14801-902, Araraquara, SP, Brazil

    Rubens Monti

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  1. Danielle Biscaro Pedrolli
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  2. Eleni Gomes
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  3. Rubens Monti
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  4. Eleonora Cano Carmona
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Correspondence to Eleonora Cano Carmona.

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Pedrolli, D.B., Gomes, E., Monti, R. et al. Studies on Productivity and Characterization of Polygalacturonase from Aspergillus giganteus Submerged Culture Using Citrus Pectin and Orange Waste. Appl Biochem Biotechnol 144, 191–200 (2008). https://doi.org/10.1007/s12010-007-8059-1

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  • DOI: https://doi.org/10.1007/s12010-007-8059-1

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