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Oxygen-controlled Biosurfactant Production in a Bench Scale Bioreactor

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Abstract

Rhamnolipids have been pointed out as promising biosurfactants. The most studied microorganisms for the aerobic production of these molecules are the bacteria of the genus Pseudomonas. The aim of this work was to produce a rhamnolipid-type biosurfactant in a bench-scale bioreactor by one strain of Pseudomonas aeruginosa isolated from oil environments. To study the microorganism growth and production dependency on oxygen, a nondispersive oxygenation device was developed, and a programmable logic controller (PLC) was used to set the dissolved oxygen (DO) concentration. Using the data stored in a computer and the predetermined characteristics of the oxygenation device, it was possible to evaluate the oxygen uptake rate (OUR) and the specific OUR (SOUR) of this microorganism. These rates, obtained for some different DO concentrations, were then compared to the bacterial growth, to the carbon source consumption, and to the rhamnolipid and other virulence factors production. The SOUR presented an initial value of about 60.0 mgO2/gDW h. Then, when the exponential growth phase begins, there is a rise in this rate. After that, the SOUR reduces to about 20.0 mgO2/gDW h. The carbon source consumption is linear during the whole process.

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Authors and Affiliations

  1. Instituto Alberto Luiz Coimbra de Pós Graduação e Pesquisa de Engenharia, Programa de Engenharia Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, bloco G, sala 115—Cidade Universitária, P.O. Box 68502, 21945-970, Rio de Janeiro, RJ, Brazil

    Frederico de Araujo Kronemberger & Cristiano Piacsek Borges

  2. Gerência de Biotecnologia e Tratamentos Ambientais, Centro de Pesquisa e Desenvolvimento Leopoldo Américo Miguêz de Mello, Cidade Universitária, Rio de Janeiro, RJ, Brazil

    Lidia Maria Melo Santa Anna

  3. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Ana Carolina Loureiro Brito Fernandes, Reginaldo Ramos de Menezes & Denise Maria Guimarães Freire

Authors
  1. Frederico de Araujo Kronemberger
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  2. Lidia Maria Melo Santa Anna
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  3. Ana Carolina Loureiro Brito Fernandes
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  4. Reginaldo Ramos de Menezes
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  5. Cristiano Piacsek Borges
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  6. Denise Maria Guimarães Freire
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Correspondence to Frederico de Araujo Kronemberger.

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Kronemberger, F.A., Santa Anna, L.M.M., Fernandes, A.C.L.B. et al. Oxygen-controlled Biosurfactant Production in a Bench Scale Bioreactor. Appl Biochem Biotechnol 147, 33–45 (2008). https://doi.org/10.1007/s12010-007-8057-3

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  • DOI: https://doi.org/10.1007/s12010-007-8057-3

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