Abstract
There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose, as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box–Behnken experimental design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 °C, to produce maximum yields of oligosaccharide.
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Acknowledgments
This research was supported by a DOE award (DE-FG36-04GO14236). This support does not constitute an endorsement by DOE of the views expressed in this article.
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Kim, M., Day, D.F. Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase. Appl Biochem Biotechnol 148, 189–198 (2008). https://doi.org/10.1007/s12010-007-8042-x
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DOI: https://doi.org/10.1007/s12010-007-8042-x