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Enzymatic Hydrolysis of Penicillin for 6-APA Production in Three-Liquid-Phase System

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Abstract

A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO–PPO–PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its “cloud point”. 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40°C) and stability (enzymatic half-time up to 55 h at 60°C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product’s side with 6-APA productivity of 80% at 42°C, pH 5.5.

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Acknowledgment

This work was financially supported by Innovation Research Group (No.20221603), National Nature Science Foundation of China (No.20490200) and the Fundamental Foundation of China (No.90610007).

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Authors and Affiliations

  1. Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing, 100080, China

    Yangyang Jiang, Hansong Xia, Chen Guo, Iram Mahmood & Huizhou Liu

  2. Graduate School of the Chinese Academy of Science, Beijing, 100039, China

    Yangyang Jiang & Iram Mahmood

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  1. Yangyang Jiang
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  2. Hansong Xia
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  3. Chen Guo
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  4. Iram Mahmood
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Correspondence to Hansong Xia or Huizhou Liu.

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Jiang, Y., Xia, H., Guo, C. et al. Enzymatic Hydrolysis of Penicillin for 6-APA Production in Three-Liquid-Phase System. Appl Biochem Biotechnol 144, 145–159 (2008). https://doi.org/10.1007/s12010-007-8018-x

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