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Separation of Cytidine 5′-triphosphate Biosynthesized from Cytidine 5′-monophosphate on Ion-exchange Resin and HPLC Analysis of Cytidine Compounds

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Abstract

Conditions were studied in the biosynthesis of cytidine 5′-triphosphate (CTP) from cytidine 5′-monophosphate (CMP). A 201 × 7 anion ion-exchange resin was applied for the separation of CTP from CMP. Adsorption isotherm and elution conditions (eluant, eluant concentration, flow rate, sample volume loaded) were investigated. At the same time, a new high-performance liquid chromatography on an anion ion-exchange column WAX-1 with UV detector at 260 nm was developed to measure CMP, cytidine 5′-diphosphate (CDP), and CTP. The retention time for CMP, CDP, and CTP are 0.723, 1.448, and 4.432 min, respectively. This new rapid high-performance liquid chromatography (HPLC) method for the analysis of cytidine compounds in biological sample has a wide linear range with high precision and repeatability.

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Authors and Affiliations

  1. College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China

    Lu-E Shi, Zhen-Xing Tang & Hua-Zhang Liu

  2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China

    Guo-Qing Ying & Yu Yi

  3. Hang Zhou Jiuyuan Gene Engineering Co., Ltd, Hangzhou, 310014, Zhejiang, China

    Jian-Feng Shan

Authors
  1. Lu-E Shi
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  2. Guo-Qing Ying
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  3. Zhen-Xing Tang
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  4. Yu Yi
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  5. Jian-Feng Shan
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  6. Hua-Zhang Liu
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Correspondence to Guo-Qing Ying.

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Shi, LE., Ying, GQ., Tang, ZX. et al. Separation of Cytidine 5′-triphosphate Biosynthesized from Cytidine 5′-monophosphate on Ion-exchange Resin and HPLC Analysis of Cytidine Compounds. Appl Biochem Biotechnol 144, 1–14 (2008). https://doi.org/10.1007/s12010-007-8015-0

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  • DOI: https://doi.org/10.1007/s12010-007-8015-0

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