Applied Biochemistry and Biotechnology

, Volume 143, Issue 1, pp 54–62 | Cite as

Accumulation of Silver(I) Ion and Diamine Silver Complex by Aeromonas SH10 biomass

  • Haoran Zhang
  • Qingbiao LiEmail author
  • Huixuan Wang
  • Daohua Sun
  • Yinghua Lu
  • Ning He


The biomass of Aeromonas SH10 was proven to strongly absorb Ag+ and [Ag(NH3)2]+. The maximum uptake of [Ag(NH3)2]+ was 0.23 g(Ag) g−1(cell dry weight), higher than that of Ag+. Fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. After SH10 cells were suspended in the aqueous solution of [Ag(NH3)2]+ under 60°C for more than 12 h, [Ag(NH3)2]+ was reduced to Ag(0), which was demonstrated by the characteristic absorbance peak of elemental silver nanoparticle in UV-VIS spectrum. Scanning electron microscopy and transmission electron microscopy observation showed that nanoparticles were formed on the cell wall after reduction. These particles were then confirmed to be elemental silver crystal by energy dispersive X-ray spectroscopy, X-ray diffraction, and UV-VIS analysis. This study demonstrated the potential use of Aeromonas SH10 in silver-containing wastewater treatment due to its high silver biosorption ability, and the potential application of bioreduction of [Ag(NH3)2]+ in nanoparticle preparation technology.


Biosorption Bioreduction Silver ion Diamine silver complex Nanoparticle 



This work is part of the project (20376076) supported by National Natural Science Foundation of China. The authors thank Analysis and Testing Center of Xiamen University for the help of SEM and TEM analysis in this study.


  1. 1.
    Hilmi, A., Luong, J., & Nguyen, A. (1999). Utilization of TiO2 deposited on glass plates for removal of metals from aqueous wastes. Chemosphere, 38, 865–874.CrossRefGoogle Scholar
  2. 2.
    Adani, K. G., Barley, R. W., & Pascoe, R. D. (2005). Silver recovery from synthetic photographic and medical X-ray process effluents using activated carbon. Mineral Engineering, 18, 1269–1276.CrossRefGoogle Scholar
  3. 3.
    Othman, N., Mat, H., & Goto, M. (2006). Separation of silver from photographic wastes by emulsion liquid membrane system. Journal of Membrane Science, 282, 171–177.CrossRefGoogle Scholar
  4. 4.
    Chen, J. P., & Lim, L. L. (2002). Key factors in chemical reduction by hydrazine for recovery of precious metals. Chemosphere, 49, 363–370.CrossRefGoogle Scholar
  5. 5.
    Pollet, B., Lorimer, J. P., Phull, S. S., & Hihn, J. Y. (2000). Sonoelectrochemical recovery of silver from photographic processing solutions. Ultrasonics Sonochemistry, 7, 69–76.CrossRefGoogle Scholar
  6. 6.
    Ajiwe, V. I. E., & Anyadiegwu, I. E. (2000). Recovery of silver from industrial wastes, cassava solution effects. Separation and Purification Technology, 18, 89–92.CrossRefGoogle Scholar
  7. 7.
    Fourest, E., Canal, C., & Roux, J. (1994). Improvement of heavy metal biosorption by mycelial dead biomasses (Rhizopus arrhizus, Mucor miehei and Penicillium chrysogenum): pH control and cationic activation. FEMS Microbiology Reviews, 14, 325–332.CrossRefGoogle Scholar
  8. 8.
    Pethkar, A. V., & Paknikar, K. M. (2003). Thiosulfate biodegradation–silver biosorption process for the treatment of photofilm processing wastewater. Process Biochemistry, 38, 855–860.CrossRefGoogle Scholar
  9. 9.
    Simmons, P., & Singleton, I. (1996). A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 45, 278–285.CrossRefGoogle Scholar
  10. 10.
    Mukherjee, P., Ahmad, A., Mandal, D., Senapati, S., Sainkar, S. R., Khan, M. I., et al. (2001). Fungus-Mediated Synthesis of Silver Nanoparticles and Their Immobilization in the Mycelial Matrix: A Novel Biological Approach to Nanoparticle Synthesis. Nano Letters, 1, 515–519.CrossRefGoogle Scholar
  11. 11.
    Ahmad, A., Mukherjee, P., Senapati, S., Mandal, D., Khan, M. I., Kumar, R., et al. (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces B, 28, 313–318.CrossRefGoogle Scholar
  12. 12.
    Klaus, T., Joerger, R., Olsson, E., & Granqvist, C. G. (1999). Silver-based crystalline nanoparticles, microbially fabricated. Proceedings of the National Academy of Sciences of the United States of America, 96, 13611–13614.CrossRefGoogle Scholar
  13. 13.
    Fu, J., Liu, Y., Gu, P., Tang, D., Lin, Z., Yao, B., et al. (2000). Spectroscopic characterization on the biosorption and bioreduction of Ag(1) by Lactobacillus sp. A09. Acta Physico-Chimica Sinica, 16, 779–782.Google Scholar
  14. 14.
    Lin, Z., Zhou, C., Wu, J., Zhou, J., & Wang, L. (2005). A further insight into the mechanism of Ag+ biosorption by Lactobacillus sp. strain A09, Spectrochimica Acta, 61, 1195–1200.CrossRefGoogle Scholar
  15. 15.
    Zhang, H., Li, Q., Lu, Y., Sun, D., Lin, X., Deng, X., et al. (2005). Biosorption and bioreduction of diamine silver complex by Corynebacterium. Journal of Chemical Technology and Biothechnology, 80, 285–290.CrossRefGoogle Scholar
  16. 16.
    Tobin, J. M., Cooper, D. G., & Neufeld, R. J. (1984). Uptake of Metal Ions by Rhizopus arrhizus Biomass. Applied and Environmental Microbiology, 47, 821–824.Google Scholar
  17. 17.
    Kapoor, S. (1998). Preparation, Characterization, and Surface Modification of Silver Particles. Langmuir, 14, 1021–1025.CrossRefGoogle Scholar
  18. 18.
    Zhu, J. J., Liu, S. W., Palchik, O., Koltypin, Y., & Gedanken, A. (2000). Shape-Controlled Synthesis of Silver Nanoparticles by Pulse Sonoelectrochemical Methods. Langmuir, 16, 6396–6399.CrossRefGoogle Scholar
  19. 19.
    Esumi, K., Hosoya, T., Suzuki, A., & Torigoe, K. (2000). Formation of Gold and Silver Nanoparticles in Aqueous Solution of Sugar-Persubstituted Poly(amidoamine) Dendrimers. Journal of Colloid and Interface Science, 226, 346–352.CrossRefGoogle Scholar
  20. 20.
    Lee, M. H., Oh, S. G., Suh, K. D., Kim, D. G., & Sohn, D. (2002). Preparation of silver nanoparticles in hexagonal phase formed by nonionic Triton X-100 surfactant. Colloids and Surfaces A, 210, 49–60.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Haoran Zhang
    • 1
  • Qingbiao Li
    • 1
    Email author
  • Huixuan Wang
    • 1
  • Daohua Sun
    • 1
  • Yinghua Lu
    • 1
  • Ning He
    • 1
  1. 1.Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Chemical Biology of Fujian ProvinceXiamen UniversityXiamenPeople’s Republic of China

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