Skip to main content
Log in

Evaluation of Enzymatic Reactors for Large-Scale Panose Production

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Panose is a trisaccharide constituted by a maltose molecule bonded to a glucose molecule by an α-1,6-glycosidic bond. This trisaccharide has potential to be used in the food industry as a noncariogenic sweetener, as the oral flora does not ferment it. Panose can also be considered prebiotic for stimulating the growth of benefic microorganisms, such as lactobacillus and bidifidobacteria, and for inhibiting the growth of undesired microorganisms such as E. coli and Samonella. In this paper, the production of panose by enzymatic synthesis in a batch and a fed-batch reactor was optimized using a mathematical model developed to simulate the process. Results show that optimum production is obtained in a fed-batch process with an optimum production of 11.23 g/l h of panose, which is 51.5% higher than production with batch reactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Tsuchiya, H. M., Hellman, N. N., Koepsell, H. J., Corman, J., Stringer, C. S., Rogovin, S. P., et al. (1955). Journal of the American Chemical Society, 77, 2412–2419.

    Article  CAS  Google Scholar 

  2. Heincke, C., Demuth, B., Jördenin, H. J., & Buchholz, K. (1999). Enzyme and Microbial Technology, 24, 523–534.

    Article  CAS  Google Scholar 

  3. Higashimura, Y., Emura, K., Kuze, N., Shirai, J., & Koda, T. (2002). Canadian Patent, 2378464.

  4. Miyake, T., Mikihiko, Y., & Kano, T. (1995). US Patent, 4518518.

  5. Machida, Y., Fukui, F., & Komoto, T. (1986). European Patent, 0242459.

  6. Chung, C. H., & Day, D. F. (2002). Journal of Industrial Microbiology & Biotechnology, 29, 196–199.

    Article  CAS  Google Scholar 

  7. Chung, C. H., & Day, D. F. (2004). Poultry Science, 83, 1302–1306.

    CAS  Google Scholar 

  8. Alsop, L. (1983). Progress in Industrial Microbiology, 18, 1–44.

    CAS  Google Scholar 

  9. Demuth, B., Jördening, H. J., & Buchholz, K. (1999). Biotechnical Engineering, 62, 583–592.

    CAS  Google Scholar 

  10. Torley, P. J., & van der Molen, F. (2005). Food Science and Technology, 38, 762–771.

    CAS  Google Scholar 

  11. Rodrigues, S. (2003). Estudo da Síntese enzimática de dextrana na presença de maltose como acceptor. Universidade Estadual de Campinas, Faculdade de Engenharia Química, Campinas, Brazil. PhD Thesis.

  12. Navarro, A., Caruel, H., & Rigal, L. (1997). Journal of Chromatography, A, 770, 39–50.

    Article  CAS  Google Scholar 

  13. Berensmeier, S., & Buchholz, K. (2004). Sep. Purific. Tech., 38, 129–138.

    Article  CAS  Google Scholar 

  14. Schulte, M., & Strube, J. (2001). Journal of Chromatograph A, 906, 399–416.

    Article  CAS  Google Scholar 

  15. Heuer, C., Kusters, E., Plattner, T., & Seidel-Morgenstern, A. (1998). Journal of Chromatograph A, 827, 175–191.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Fabiano A. N. Fernandes.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernandes, F.A.N., Rodrigues, S. Evaluation of Enzymatic Reactors for Large-Scale Panose Production. Appl Biochem Biotechnol 142, 95–104 (2007). https://doi.org/10.1007/s12010-007-0046-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-007-0046-z

Keywords

Navigation