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Decolorization of Some Azo Dyes by Immobilized Geotrichum sp. Biomass in Fluidized Bed Bioreactor

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Abstract

Geotrichum sp. strain, which is able to decolorize azo dyes enzymatically, was used in this study for decolorization of synthetics solutions contaminated by toxic azo dyes orange G, trypan blue, azorubine, and methyl red. The biomass of Geotrichum sp. was immobilized in calcium alginate and polyacrylamide gels and used for the decolorization of tested azo dyes in fluidized bed bioreactor. The highest specific decolorization rate was obtained when the fungal biomass was entrapped in calcium alginate beads. Immobilized biomass in calcium alginate continuously decolorized azo dyes after eight repeated batch decolorization experiments without significant loss of activity whereas polyacrylamide immobilized biomass retained only 10% of its activity after 4 days of incubation. The effects of some physicochemical parameters such as temperature, pH, and dyes concentration on decolorization performance of isolated fungal strain were also investigated.

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Acknowledgements

This work was supported by the grant of 2005 Postdoctoral Program and research funds of Chonbuk National University and the Oriental Medicine R&D Project, Ministry of Health and Welfare, Republic of Korea (0405-OM00-0815-0001).

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Authors and Affiliations

  1. Laboratory of Enzyme Technology, College of Natural Science, Chonbuk National University, Chonju, 561-756, Republic of Korea

    Youssef Zeroual, Beom Su Kim, Myoung Won Yang & Kang Min Lee

  2. Laboratory of Microbiology, Biotechnology and Environment, Faculty of Sciences, University Hassan II, Cassablanca, Morocco

    Youssef Zeroual & Mohamed Blaghen

Authors
  1. Youssef Zeroual
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  2. Beom Su Kim
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  3. Myoung Won Yang
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  4. Mohamed Blaghen
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  5. Kang Min Lee
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Correspondence to Kang Min Lee.

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Zeroual, Y., Kim, B.S., Yang, M.W. et al. Decolorization of Some Azo Dyes by Immobilized Geotrichum sp. Biomass in Fluidized Bed Bioreactor. Appl Biochem Biotechnol 142, 307–316 (2007). https://doi.org/10.1007/s12010-007-0037-0

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  • DOI: https://doi.org/10.1007/s12010-007-0037-0

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