Applied Biochemistry and Biotechnology

, Volume 143, Issue 1, pp 93–100 | Cite as

A Novel Stepwise Recovery Strategy of Cellulase Adsorbed to the Residual Substrate after Hydrolysis of Steam Exploded Wheat Straw

  • Jian Xu
  • Hongzhang ChenEmail author


Cellulase distribution between residual substrate and supernatant in the process of enzymatic hydrolysis of steam-exploded wheat straw was investigated. Subsequently, a novel stepwise recovery strategy with three successive steps was adopted to recover cellulase adsorbed to the residual substrate. The results showed that cellulase protein in the supernatant increased as the hydrolysis time increased. When hydrolysis ended, the cellulase remaining on the residual substrate accounted for 33–42% of the original added cellulase according to the different cellulase loading. To obtain the maximum cellulase recovery rate, the residual substrate was dealt with in three successive steps: washed with sodium acetate buffer (step 1), shaken with sodium acetate buffer (step 2), and then treated with 0.0015 mol/L, pH 10 Ca(OH)2 (step 3). The total cellulase protein recovered by the three steps reached 96.70–98.14%. The enzyme activity of cellulase recovered by the first two steps was kept well. The ratios of the specific activity between the recovered cellulase and the original were 89–96%, which was by far higher than that using step 3 (the value was 48% ∼ 56%).


Cellulase Enzymatic hydrolysis Residual substrate Stepwise recovery strategy Specific activity 



Financial support for this study was provided by National Basic Research Program of China (2004CB719700) and the Knowledge Innovation Program of Chinese Academy of Sciences (KJCXZ-SW-206-2).


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  1. 1.State Key Lab of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Environmental Science and ForestryState University of New YorkSyracuseUSA

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