Abstract
Laccase production by solid-state fermentation (SSF) using an indigenously isolated white rot basidiomycete Ganoderma sp. was studied. Among the various agricultural wastes tested, wheat bran was found to be the best substrate for laccase production. Solid-state fermentation parameters such as optimum substrate, initial moisture content, and inoculum size were optimized using the one-factor-at-a-time method. A maximum laccase yield of 2,400 U/g dry substrate (U/gds) was obtained using wheat bran as substrate with 70% initial moisture content at 25°C and the seven agar plugs as the inoculum. Further enhancement in laccase production was achieved by supplementing the solid-state medium with additional carbon and nitrogen source such as starch and yeast extract. This medium was optimized by response surface methodology, and a fourfold increase in laccase activity (10,050 U/g dry substrate) was achieved. Thus, the indigenous isolate seems to be a potential laccase producer using SSF. The process also promises economic utilization and value addition of agro-residues.
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References
Bajpai, P. (1999). Biotechnology Progress, 15, 147–157.
Bollag, J. M., & Leonowicz, A. (1984). Applied and Environmental Microbiology, 48(4), 849–854.
Breen, A., & Singleton, F. L. (1999). Current Opinion in Biotechnology, 10, 252–258.
Gu, X. B., Zheng, Z. M., Yu, H. Q., Wang, J., Liang, F. L., & Liu, R. L. (2005). Process Biochemistry, 40, 3196–3201.
Higuchi, T. (1990). Wood Science and Technology, 24, 23–63.
Karam, J., & Nicell, J. A. (1997). Journal of Chemistry Technology and Biotechnology, 69, 141–153.
Lorenzo, M., Moldes, D., Rodriguez Couto, S., & Sanroman, A. (2002). Bioresource Technology, 82, 109–113.
Machuca, A., Aoyama, H., & Duran, N. (1998). Biotechnology and Applied Biochemistry, 27, 217–223.
Myers, R. H., & Montgomery, D. C. (1995). Response surface methodology: Process and product optimization using designed experiments. New York, NY: Wiley.
Niku Paavola, M. L., Karhunen, E., Salola, P., & Raunio, V. (1988). Biochemical Journal, 254, 877–884.
Nyanhongo, G. S., Gomes, J., Gubitz, G., Zvauya, R., Read, J. S., & Steiner, W. (2002). Bioresource Technology, 84, 259–263.
Orth, A. B., Royse, D. J., & Tien, M. (1993). Applied and Environmental Microbiology, 59, 4017–4083.
Pandey, A. (1992). Process Biochemistry, 7, 109–116.
Pandey, A. (Ed.) (1994). Solid state fermentation. New Delhi: Wiley Eastern Limited.
Pandey, A., Selvakumar, P., Soccol, C. R., & Nigam, P. (1999). Current Science, 77, 149–162.
Paszczynski, A., & Crawford, R. L. (1995). Biotechnology Progress, 11, 368–379.
Reddy, C. A. (1995). Current Opinion in Biotechnology, 6, 320–328.
Revankar, M. S., & Lele, S. S. (2006). Process Biochemistry, 41(3), 581–588.
Sabu, A., Pandey, A., Daud, M. J., & Szakacs, G. (2005). Bioresource Technology, 96, 1223–1228.
Shah, V., & Nerud, F. (2002). Canadian Journal of Microbiology, 48, 857–870.
Techapun, C., Charoenrat, T., Watanabe, M., Sasaki, K., & Poosaran, N. (2002). Biochemical Journal, 12, 99–105.
Thurston, C. F. (1994). Microbiology, 140, 19–26.
Vasconcelos, A. D., Barbosa, A. M., Dekker, R. F. H., Scarminio, I. S., & Rezende, M. I. (2000). Process Biochemistry, 35, 1131–1138.
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Revankar, M.S., Desai, K.M. & Lele, S.S. Solid-state Fermentation for Enhanced Production of Laccase using Indigenously Isolated Ganoderma sp.. Appl Biochem Biotechnol 143, 16–26 (2007). https://doi.org/10.1007/s12010-007-0029-0
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DOI: https://doi.org/10.1007/s12010-007-0029-0