Abstract
The increase in metal-on-metal hip arthroplasties has led to concern regarding the effect of raised serum and tissue metal ion levels. Our aim was to determine changes in the integrity and function of cells of the immune system after exposure to CoCr nanoparticles in specific cell culture experiments. Nanometer-sized particles of CoCr were made from a manufacturer’s forged CoCr used for metal-on-metal articulations. Primary, murine dendritic cells and T and B lymphocytes then were exposed to these CoCr particles under cell culture conditions and then assayed for viability and proliferation/activation. CoCr nanoparticles did not directly activate dendritic cells or regulate B cells. Although nanoparticles were not directly toxic to resting T cells, Signals 1- and 2-dependent T cell proliferation were reduced. This may explain the observed reduction in CD8+ T cells observed in patients with metal-on-metal implants.
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The help and expertise of N. S. Tabrizi in producing the nanoparticles is greatly appreciated.
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Each author certifies that he or she has no commercial associations (eg, consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted article.
Each author certifies that his or her institution has approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.
This study was done at Southern General Hospital, Glasgow, Scotland, Centre for Biophotonics, SIPBS, University of Strathclyde, Glasgow, UK, and Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands.
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Ogunwale, B., Schmidt-Ott, A., Meek, R.M.D. et al. Investigating the Immunologic Effects of CoCr Nanoparticles. Clin Orthop Relat Res 467, 3010–3016 (2009). https://doi.org/10.1007/s11999-009-0949-9
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DOI: https://doi.org/10.1007/s11999-009-0949-9