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Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging

  • Symposium: New Approaches to Allograft Transplantation
  • Published:
Clinical Orthopaedics and Related Research

Abstract

Ionizing radiation could supplement tissue bank screening to further reduce the probability of diseases transmitted by allografts if denaturation effects can be minimized. It is important, however, such sterilization procedures be nondetrimental to tissues. We compared crosslinking and free radical scavenging potential methods to accomplish this task in tendon tissue. In addition, two forms of ionizing irradiation, gamma and electron beam (e-beam), were also compared. Crosslinkers included 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and glucose, which were used to add exogenous crosslinks to collagen. Free radical scavengers included mannitol, ascorbate, and riboflavin. Radioprotective effects were assessed through tensile testing and collagenase resistance testing after irradiation at 25 kGy and 50 kGy. Gamma and e-beam irradiation produced similar degenerative effects. Crosslinkers had the highest strength at 50 kGy, EDC treated tendons had 54% and 49% higher strength than untreated, for gamma and e-beam irradiation respectively. Free radical scavengers showed protective effects up to 25 kGy, especially for ascorbate and riboflavin. Crosslinked samples had higher resistance to collagenase and over a wider dose range than scavenger-treated. Of the options studied, the data suggest EDC precrosslinking or glucose treatment provides the best maintenance of native tendon properties after exposure to ionizing irradiation.

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Authors and Affiliations

  1. Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, UMDNJ-Robert Wood Johnson Medical School, 51 French Street, Orthopaedic Surgery, Room 424, PO Box 19, New Brunswick, NJ, 08903, USA

    Aaron Seto MS, Charles J. Gatt Jr MD & Michael G. Dunn PhD

Authors
  1. Aaron Seto MS
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  2. Charles J. Gatt Jr MD
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  3. Michael G. Dunn PhD
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Corresponding author

Correspondence to Michael G. Dunn PhD.

Additional information

One or more of the authors (MGD) have received funding from the Musculoskeletal Transplant Foundation Peer Reviewed Scientific Grants Program (January–December 2005).

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Seto, A., Gatt, C.J. & Dunn, M.G. Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging. Clin Orthop Relat Res 466, 1788–1795 (2008). https://doi.org/10.1007/s11999-008-0301-9

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  • DOI: https://doi.org/10.1007/s11999-008-0301-9

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