In agreement with the recent trend of consumers’ demand for natural antimicrobial products, the importance of using natural compounds in food is increasing. Among numerous natural compounds, grapefruit seed extract (GSE) is widely known for its extremely effective antimicrobial activity. The direct use of GSE in food is the most common method of application. Other GSE applications exist using it with coating materials and incorporating it into edible films. Previously done studies identified benzalkonium chloride, benzethonium chloride, and other synthetic agents from GSE. Unfortunately, the presence of these antibacterial contents does not exactly coincide with the natural aspects of GSE. Even though GSE is identified as a natural antimicrobial, this observation suggests that the GSE antibacterial activity is derived from these synthetic substances such as benzalkonium chloride. Therefore, further research is needed about these controversial issues. This review analyzed the application of GSE as a natural antimicrobial in the food industry and suggested further research directions.
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Aloui, H., & Khwaldia, K. (2016). Natural antimicrobial edible coatings for microbial safety and food quality enhancement. Comprehensive Reviews in Food Science and Food Safety, 15(6), 1080–1103.
Aloui, H., Khwaldia, K., Sánchez-González, L., Muneret, L., Jeandel, C., Hamdi, M., & Desobry, S. (2014). Alginate coatings containing grapefruit essential oil or grapefruit seed extract for grapes preservation. International Journal of Food Science & Technology, 49(4), 952–959.
Armando, C., Maythe, S., & Beatriz, N. P. (1998). Antioxidant activity of grapefruit seed extract on vegetable oils. Journal of the Science of Food and Agriculture, 77(4), 463–467.
Avula, B., Dentali, S., & Khan, I. A. (2007). Simultaneous identification and quantification by liquid chromatography of benzethonium chloride, methyl paraben and triclosan in commercial products labeled as grapefruit seed extract. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 62(8), 593–596.
Bekiroglu, S., Myrberg, O., Östman, K., Ek, M., Arvidsson, T., Rundlöf, T., & Hakkarainen, B. (2008). Validation of a quantitative NMR method for suspected counterfeit products exemplified on determination of benzethonium chloride in grapefruit seed extracts. Journal of Pharmaceutical and Biomedical Analysis, 47(4-5), 958–961.
Callaway, T. R., Carroll, J. A., Arthington, J. D., Edrington, T. S., Anderson, R. C., Ricke, S. C., et al. (2011). Citrus products and their use against bacteria: Potential health and cost benefits. In Nutrients, dietary supplements, and nutriceuticals (pp. 277–286). Humana Press.
Calo, J. R., Crandall, P. G., O'Bryan, C. A., & Ricke, S. C. (2015). Essential oils as antimicrobials in food systems–A review. Food Control, 54, 111–119.
Cho, S. H., Seo, I. W., Choi, J. D., & Joo, I. S. (1990). Antimicrobial and antioxidant activity of grapefruit and seed extract on fishery products. Korean Journal of Fisheries and Aquatic Sciences, 23(4), 289–296.
Cho, S. H., Lee, H. C., Seo, I. W., Kim, Z. U., Chang, Y. S., & Shin, Z. I. (1991). Efficacy of grapefruit seed extract in the preservation of Satsuma mandarin. Korean Journal of Food Science and Technology, 23(5), 614–618.
Choi, J. S., Lee, Y. R., Ha, Y. M., Seo, H. J., Kim, Y. H., Park, S. M., & Sohn, J. H. (2014). Antibacterial effect of grapefruit seed extract (GSE) on Makgeolli-brewing microorganisms and its application in the preservation of fresh Makgeolli. Journal of Food Science, 79(6), M1159–M1167.
Cvetnić, Z., & Vladimir-Knežević, S. (2004). Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharmaceutica, 54(3), 243–250.
Dabbah, R., Edwards, V. M., & Moats, W. A. (1970). Antimicrobial action of some citrus fruit oils on selected food-borne bacteria. Applied Microbiology, 19(1), 27–31.
Dehghani, S., Hosseini, S. V., & Regenstein, J. M. (2018). Edible films and coatings in seafood preservation: A review. Food Chemistry, 240, 505–513.
Del Nobile, M. A., Lucera, A., Costa, C., & Conte, A. (2012). Food applications of natural antimicrobial compounds. Frontiers in Microbiology, 3, 287.
Dhall, R. K. (2013). Advances in Edible Coatings for Fresh Fruits and Vegetables: A Review. Critical Reviews in Food Science and Nutrition, 53(5), 435–450.
Faleiro, M. L. (2011). The mode of antibacterial action of essential oils. Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, 2, 1143–1156.
Ganzera, M., Aberham, A., & Stuppner, H. (2006). Development and validation of an HPLC/UV/MS method for simultaneous determination of 18 preservatives in grapefruit seed extract. Journal of Agricultural and Food Chemistry, 54(11), 3768–3772.
Gonelimali, F. D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S. R. (2018). Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers in Microbiology, 9, 1639.
Gyawali, R., & Ibrahim, S. A. (2014). Natural products as antimicrobial agents. Food Control, 46, 412–429.
Haskaraca, G., Juneja, V. K., Mukhopadhyay, S., & Kolsarici, N. (2019). Food Control, 95, 71–76.
Hong, Y. H., Lim, G. O., & Song, K. B. (2009). Physical properties of Gelidium corneum–gelatin blend films containing grapefruit seed extract or green tea extract and its application in the packaging of pork loins. Journal of Food Science, 74(1), C6–C10.
Jang, S. A., Shin, Y. J., & Song, K. B. (2011). Effect of rapeseed protein–gelatin film containing grapefruit seed extract on ‘Maehyang’ strawberry quality. International Journal of Food Science & Technology, 46(3), 620–625.
Jianglian, D., & Shaoying, Z. (2013). Application of chitosan based coating in fruit and vegetable preservation: A review. Journal of Food Processing & Technology, 4(5), 227.
Jo, W. S., Song, N. B., Lee, J. H., & Song, K. B. (2014). Physical properties and antimicrobial activities of a persimmon peel/red algae composite film containing grapefruit seed extract. Food Science and Biotechnology, 23(4), 1169–1172.
Juneja, V. K., Fan, X., Peña-Ramos, A., Diaz-Cinco, M., & Pacheco-Aguilar, R. (2006). The effect of grapefruit extract and temperature abuse on growth of Clostridium perfringens from spore inocula in marinated, sous-vide chicken products. Innovative Food Science & Emerging Technologies, 7(1-2), 100–106.
Kanmani, P., & Rhim, J. W. (2014a). Antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract. Carbohydrate Polymers, 102, 708–716.
Kanmani, P., & Rhim, J. W. (2014b). Development and characterization of carrageenan/grapefruit seed extract composite films for active packaging. International Journal of Biological Macromolecules, 68, 258–266.
Kim, J. W., Matsler, P. L., Wang, H., & Slavik, M. F. (1996). Grapefruit seed extract (BF-100) treatment of poultry to reduce attached Salmonella. Journal of Food Hygiene and Safety, 11(1).
Kim, J. H., Kwon, K. H., & Oh, S. W. (2016). Effects of malic acid or/and grapefruit seed extract for the inactivation of common food pathogens on fresh-cut lettuce. Food Science and Biotechnology, 25(6), 1801–1804.
Kim, J. H., Hong, W. S., & Oh, S. W. (2018). Effect of layer-by-layer antimicrobial edible coating of alginate and chitosan with grapefruit seed extract for shelf-life extension of shrimp (Litopenaeus vannamei) stored at 4 C. International Journal of Biological Macromolecules, 120(Pt B), 1468–1473.
Kumar, S., Mukherjee, A., & Dutta, J. (2020). Chitosan based nanocomposite films and coatings: Emerging antimicrobial food packaging alternatives. Trends in Food Science & Technology, 97, 196–209.
Misir, J., Brishti, F. H., & Hoque, M. M. (2014). Aloe vera gel as a novel edible coating for fresh fruits: A review. American Journal of Food Science and Technology, 2(3), 93–97.
Muthaiyan, A., Martin, E. M., Natesan, S., Crandall, P. G., Wilkinson, B. J., & Ricke, S. C. (2012). Antimicrobial effect and mode of action of terpeneless cold-pressed Valencia orange essential oil on methicillin-resistant Staphylococcus aureus. Journal of Applied Microbiology, 112(5), 1020–1033.
Perez-Perez, C., Regalado-González, C., Rodríguez-Rodríguez, C. A., Barbosa-Rodríguez, J. R., & Villaseñor-Ortega, F. (2006). Incorporation of antimicrobial agents in food packaging films and coatings. Advances in Agricultural and Food Biotechnology, 11, 193–216.
Raghav, P. K., Agarwal, N., & Saini, M. (2016). Edible coating of fruits and vegetables: A review. International Journal of Scientific Research and Modern Education, 1(1), 188–204.
Reagor, L., Gusman, J., McCoy, L., Carino, E., & Heggers, J. P. (2002). The effectiveness of processed grapefruit-seed extract as an antibacterial agent: I. An in vitro agar assay. The Journal of Alternative & Complementary Medicine, 8(3), 325–332.
Sakamoto, S., Sato, K., Maitani, T., & Yamada, T. (1996). Analysis of components in natural food additive “grapefruit seed extract” by HPLC and LC/MS. Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences, (114), 38–42.
Shankar, S., & Rhim, J. W. (2018). Antimicrobial wrapping paper coated with a ternary blend of carbohydrates (alginate, carboxymethyl cellulose, carrageenan) and grapefruit seed extract. Carbohydrate Polymers, 196, 92–101.
Shin, Y. J., Song, H. Y., Seo, Y. B., & Song, K. B. (2012). Preparation of red algae film containing grapefruit seed extract and application for the packaging of cheese and bacon. Food Science and Biotechnology, 21(1), 225–231.
Shin, Y. J., Song, H. Y., Jo, W. S., Lee, M. J., & Song, K. B. (2013). Physical properties of a barley protein/nano-clay composite film containing grapefruit seed extract and antimicrobial benefits for packaging of A garicus bisporus. International Journal of Food Science & Technology, 48(8), 1736–1743.
Sofos, J. N., Beuchat, L. R., Davidson, P. M., & Johnson, E. A. (1998). Naturally occurring antimicrobials in food. Regulatory Toxicology and Pharmacology: RTP, 28(2), 71–72.
Song, H. Y., Shin, Y. J., & Song, K. B. (2012). Preparation of a barley bran protein–gelatin composite film containing grapefruit seed extract and its application in salmon packaging. Journal of Food Engineering, 113(4), 541–547.
Tahir, H. E., Xiaobo, Z., Mahunu, G. K., Arslan, M., Abdalhai, M., & Zhihua, L. (2019). Recent developments in gum edible coating applications for fruits and vegetables preservation: A review. Carbohydrate Polymers, 224, 115141.
Takeoka, G., Dao, L., Wong, R. Y., Lundin, R., & Mahoney, N. (2001). Identification of benzethonium chloride in commercial grapefruit seed extracts. Journal of Agricultural and Food Chemistry, 49(7), 3316–3320.
Takeoka, G. R., Dao, L. T., Wong, R. Y., & Harden, L. A. (2005). Identification of benzalkonium chloride in commercial grapefruit seed extracts. Journal of Agricultural and Food Chemistry, 53(19), 7630–7636.
Valencia-Chamorro, S. A., Palou, L., del Rio, M. A., & Perez-Gago, M. B. (2011). Antimicrobial edible films and coatings for fresh and minimally processed fruits and vegetables: A review. Critical Reviews in Food Science and Nutrition, 51(9), 872–900.
Von Woedtke, T., Schlüter, B., Pflegel, P., Lindequist, U., & Jülich, W. D. (1999). Aspects of the antimicrobial efficacy of grapefruit seed extract and its relation to preservative substances contained. Die Pharmazie, 54(6), 452–456.
Wang, L. F., & Rhim, J. W. (2016). Grapefruit seed extract incorporated antimicrobial LDPE and PLA films: Effect of type of polymer matrix. LWT, 74, 338–345.
Wang, K., Lim, P. N., Tong, S. Y., & San Thian, E. (2019). Development of grapefruit seed extract-loaded poly (ε-caprolactone)/chitosan films for antimicrobial food packaging. Food Packaging and Shelf Life, 22, 100396.
Won, J. S., Lee, S. J., Park, H. H., Song, K. B., & Min, S. C. (2018). Edible coating using a chitosan-based colloid incorporating grapefruit seed extract for cherry tomato safety and preservation. Journal of Food Science, 83(1), 138–146.
Xu, W. T., Huang, K. L., Guo, F., Qu, W., Yang, J. J., Liang, Z. H., & Luo, Y. B. (2007a). Postharvest grapefruit seed extract and chitosan treatments of table grapes to control Botrytis cinerea. Postharvest Biology and Technology, 46(1), 86–94.
Xu, W., Qu, W., Huang, K., Guo, F., Yang, J., Zhao, H., & Luo, Y. (2007b). Antibacterial effect of grapefruit seed extract on food-borne pathogens and its application in the preservation of minimally processed vegetables. Postharvest Biology and Technology, 45(1), 126–133.
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Kim, T., Kim, JH. & Oh, SW. Grapefruit Seed Extract as a Natural Food Antimicrobial: a Review. Food Bioprocess Technol 14, 626–633 (2021). https://doi.org/10.1007/s11947-021-02610-5
- Foodborne pathogen
- Food safety
- Grapefruit seed extract