Food and Bioprocess Technology

, Volume 12, Issue 7, pp 1197–1204 | Cite as

Ethanol Precipitation of Mannooligosaccharides from Subcritical Water-Treated Coconut Meal Hydrolysate

  • Khwanjai Klinchongkon
  • Thapanee Bunyakiat
  • Pramote KhuwijitjaruEmail author
  • Shuji Adachi
Original Paper


Subcritical water hydrolysis is an effective method for producing mannooligosaccharides from coconut meal, which is a by-product from coconut milk processing. In this study, the purification process to obtain mannooligosaccharides from coconut meal hydrolysate was investigated. The effects of adsorbent (activated carbon and bentonite), concentration (1–10% w/v), and adsorption time (5–60 min) were studied for impurities removal. The activated carbon showed much higher efficiency for impurities removal. Mannooligosaccharides were precipitated using ethanol at different concentrations (0–90% v/v) and initial carbohydrate contents (50, 100, and 200 g/L). The results showed that the ethanol concentration at 90% v/v and initial carbohydrate content of 200 g/L gave the highest recovery of saccharides (31 g/L). The obtained precipitate contained 9.7, 22.6, 12.9, 19.4, 19.4, and 16.1% w/w of saccharides with 1 to 6 degree of polymerization, respectively.


Mannooligosaccharide recovery Oligosaccharide purification Cocos nucifera L. Subcritical water treatment Subcritical water hydrolysis 


Funding information

This research was partly funded by a grant from Silpakorn University Research and development Institute [SURDI 53/02/03].


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Innovation in Food Technology, College of Health SciencesChristian University of ThailandNakhon PathomThailand
  2. 2.Department of Food Technology, Faculty of Engineering and Industrial TechnologySilpakorn UniversityNakhon PathomThailand
  3. 3.Department of Agriculture and Food Technology, Faculty of Bio-environmental ScienceKyoto University of Advanced ScienceKyotoJapan

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