Construction of a Biocompatible and Antioxidant Multilayer Coating by Layer-by-Layer Assembly of κ-Carrageenan and Quercetin Nanoparticles

Souza, Marthyna P.; Vaz, Antônio F. M.; Costa, Thacianna B.; Cerqueira, Miguel A.; De Castro, Célia M. M. B.; Vicente, António A.; Carneiro-da-Cunha, Maria G.

doi:10.1007/s11947-018-2077-6

Original Paper
Published: 17 February 2018

Construction of a Biocompatible and Antioxidant Multilayer Coating by Layer-by-Layer Assembly of κ-Carrageenan and Quercetin Nanoparticles

Marthyna P. Souza^1,2,3,
Antônio F. M. Vaz⁴,
Thacianna B. Costa²,
Miguel A. Cerqueira³^nAff5,
Célia M. M. B. De Castro²,
António A. Vicente³ &
Maria G. Carneiro-da-Cunha ORCID: orcid.org/0000-0002-7219-5527^1,2

Food and Bioprocess Technology volume 11, pages 1050–1060 (2018)Cite this article

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Abstract

The present work aimed at the construction and characterization of a multilayer coating based on κ-carrageenan and quercetin-loaded lecithin/chitosan nanoparticles (Np) by the layer-by-layer technique and the evaluation of its antioxidant capacity and potential cytotoxicity in vitro. The multilayered coating was successfully self-assembled, as confirmed by UV-Vis spectroscopy, contact angle, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Multilayered coatings showed to have antioxidant capacity, with a DPPH• radical scavenging activity of 31.32 ± 3.13% and a result of the FRAP assay of 799.41 ± 95.39 μM of ferrous ion (Fe²⁺) equivalent. These coatings were also shown to be devoid of cell toxicity, as evaluated by determination of nitric oxide production and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. The alveolar macrophages culture was tested in the presence of the κ-carrageenan/quercetin-Np multilayer coating and showed a cell viability of 91.3 ± 9.6%. These results suggest that this multilayered coating is adequate for surfaces modification in view of biomedical and food industry applications.

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Acknowledgements

This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors would also like to thank the Brazilian Government for support given by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Carneiro-da-Cunha, M.G. expresses her gratitude to the CNPq for research grant.

Author information

Miguel A. Cerqueira
Present address: International Iberian Nanotechnology Laboratory-INL, Av. Mestre José Veiga s/n, 4715-330, Braga, Portugal

Authors and Affiliations

Biochemistry Department, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50, Recife, Pernambuco, 670-420, Brazil
Marthyna P. Souza & Maria G. Carneiro-da-Cunha
LIKA - Laboratório de Imunopatologia Keizo Asami, UFPE, Cidade Universitaria, CEP 50, Recife, Pernambuco, 670-901, Brazil
Marthyna P. Souza, Thacianna B. Costa, Célia M. M. B. De Castro & Maria G. Carneiro-da-Cunha
CEB – Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
Marthyna P. Souza, Miguel A. Cerqueira & António A. Vicente
Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande, CEP 58, Patos, Pernambuco, 7000-970, Brazil
Antônio F. M. Vaz

Authors

Marthyna P. Souza
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Antônio F. M. Vaz
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Thacianna B. Costa
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Miguel A. Cerqueira
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Célia M. M. B. De Castro
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António A. Vicente
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Maria G. Carneiro-da-Cunha
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Correspondence to Maria G. Carneiro-da-Cunha.

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Souza, M.P., Vaz, A.F.M., Costa, T.B. et al. Construction of a Biocompatible and Antioxidant Multilayer Coating by Layer-by-Layer Assembly of κ-Carrageenan and Quercetin Nanoparticles. Food Bioprocess Technol 11, 1050–1060 (2018). https://doi.org/10.1007/s11947-018-2077-6

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Received: 27 September 2017
Accepted: 02 February 2018
Published: 17 February 2018
Issue Date: May 2018
DOI: https://doi.org/10.1007/s11947-018-2077-6

Keywords

Biomaterial
Cytotoxicity
Multilayer
Polyelectrolyte
Surface modification