Functional Properties of Plasticized Bio-Based Poly(Lactic Acid)_Poly(Hydroxybutyrate) (PLA_PHB) Films for Active Food Packaging
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Fully bio-based and biodegradable active films based on poly(lactic acid) (PLA) blended with poly(3-hydroxybutyrate) (PHB) and incorporating lactic acid oligomers (OLA) as plasticizers and carvacrol as active agent were extruded and fully characterized in their functional properties for antimicrobial active packaging. PLA_PHB films showed good barrier to water vapor, while the resistance to oxygen diffusion decreased with the addition of OLA and carvacrol. Their overall migration in aqueous food simulant was determined and no significant changes were observed by the addition of carvacrol and OLA to the PLA_PHB formulations. However, the effect of both additives in fatty food simulant can be considered a positive feature for the potential protection of foodstuff with high fat content. Moreover, the antioxidant and antimicrobial activities of the proposed formulations increased by the presence of carvacrol, with enhanced activity against Staphylococcus aureus if compared to Escherichia coli at short and long incubation times. These results underlined the specific antimicrobial properties of these bio-films suggesting their applicability in active food packaging.
KeywordsBio-films Active packaging Lactic acid oligomers Carvacrol Migration Antibacterial properties
This work was funded by the SAMSUNG GRO PROGRAMME 2012 and the Spanish Ministry of Economy and Competitiveness (Ref. MAT2014-59242-C2-2-R and MAT2014-55778-REDT).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Arrieta, M. P., Castro-López, M. M., Rayón, E., Barral-Losada, L. F., López-Vilariño, J. M., López, J., et al. (2014a). Plasticized poly (lactic acid)–poly (hydroxybutyrate) (PLA–PHB) blends incorporated with Catechin intended for active food-packaging applications. Journal of Agricultural and Food Chemistry, 62(41), 10170–10180.CrossRefGoogle Scholar
- ASTM (2005). Standard test methods for water vapor transmission of materials. In ASTM E-96/E 96 M-05: American Society for Testing and Materials.Google Scholar
- CLSI (2015). Performance standards for antimicrobial disk susceptibility tests; approved standard_Twelfth edition in CLSI document MO2-A12 Wayne. PA: Clinical and Laboratory Standards Institute.Google Scholar
- Cristani, M., D’Arrigo, M., Mandalari, G., Castelli, F., Sarpietro, M. G., Micieli, D., et al. (2007). Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. Journal of Agricultural and Food Chemistry, 55(15), 6300–6308.CrossRefGoogle Scholar
- Chaiwutthinan, P., Pimpan, V., Chuayjuljit, S., & Leejarkpai, T. (2015). Biodegradable plastics prepared from poly (lactic acid), poly (butylene succinate) and microcrystalline cellulose extracted from waste-cotton fabric with a chain extender. Journal of Polymers and the Environment, 23(1), 114–125.CrossRefGoogle Scholar
- Di Pasqua, R., Hoskins, N., Betts, G., & Mauriello, G. (2006). Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, Cinnamaldehyde, and eugenol in the growing media. Journal of Agricultural and Food Chemistry, 54(7), 2745–2749.CrossRefGoogle Scholar
- EC (2002). Commission Directive 2002/72/EC relating to plastic materials and articles intended to come into contact with foodstuffs. In Official Journal of European Communities.Google Scholar
- EC (2011). Commission Regulation EU N° 10/2011 on plastic materials and articles intended to come into contact with food. In Official Journal of European Communities.Google Scholar
- Fiori, S., & Ara, P. (2009). Method for plasticizing lactic acid polymers. World Patent.Google Scholar
- ISO (2004). Plastics. Determination of the degree of disintegration of plastic materials under simulated composting conditions in a laboratory-scale test. In ISO 20200:2004: International Organization for Standardization.Google Scholar
- Mastelić, J., Jerković, I., Blažević, I., Poljak-Blaži, M., Borović, S., Ivančić-Baće, I., et al. (2008). Comparative study on the antioxidant and biological activities of carvacrol, thymol, and eugenol derivatives. Journal of Agricultural and Food Chemistry, 56(11), 3989–3996.CrossRefGoogle Scholar
- Salmieri, S., Islam, F., Khan, R. A., Hossain, F. M., Ibrahim, H. M. M., Miao, C., et al. (2014). Antimicrobial nanocomposite films made of poly (lactic acid)-cellulose nanocrystals (PLA-CNC) in food applications: part A—effect of nisin release on the inactivation of Listeria monocytogenes in ham. Cellulose, 21(3), 1837–1850.CrossRefGoogle Scholar
- Ultee, A., Kets, E. P. W., & Smid, E. J. (1999). Mechanisms of action of carvacrol on the food-borne pathogen. Applied and Environmental Microbiology, 65(10), 4606–4610.Google Scholar