Food and Bioprocess Technology

, Volume 10, Issue 4, pp 630–638 | Cite as

A Non-invasive Real-Time Methodology for the Quantification of Antioxidant Properties in Coffee During the Roasting Process Based on Near-Infrared Spectroscopy

  • Tiago A. Catelani
  • Ricardo N. M. J. Páscoa
  • João Rodrigo Santos
  • Leonardo Pezza
  • Helena R. Pezza
  • José L. F. C. Lima
  • João A. Lopes
Original Paper


This paper describes the development of a real-time method based on near-infrared spectroscopy (NIRS) for determination of total antioxidant capacity (TAC) and total phenolic content (TPC) in coffee during the roasting process. The real-time non-invasive monitoring procedure involved pointing a diffuse reflectance probe directly at the roasting chamber through a glass window in order to monitor the roasting process. The figures of merit of the chemometric models to estimate TAC and TPC showed selectivity values higher than 12% and determination coefficients (R 2 P) above 0.90. The TAC and TPC profiles during the roasting procedure are discussed in terms of the antioxidant compounds likely to be present in green and roasted coffee. NIRS was found to be a satisfactory real-time tool for monitoring the content of antioxidant compounds in coffee during the roasting process, complementing other established procedures.


Near-infrared spectroscopy Coffee roasting Real-time monitoring Total antioxidant capacity Total phenolic content 



T.A. Catelani is grateful to CAPES for a scholarship (PDSE no. 99999.000655/2015-05) and to CNPq. R.N.J. Páscoa acknowledges financial support from SFRH/BPD/81384/2011. This work received financial support from FCT/MEC through national funds and was co-financed by FEDER under partnership agreement PT2020—UID/QUI/50006/2013—POCI/01/0145/FEDER/007265. The authors also acknowledge funding from NORTE-01-0145-FEDER-000011 and iMed.ULisboa (grant UID/DTP/04138/2013).

Supplementary material

11947_2016_1843_MOESM1_ESM.docx (156 kb)
Fig. SD1 Score plot from a PCA model build with near-infrared spectra of roasted coffee samples of four geographical origins. (DOCX 155 kb)
11947_2016_1843_MOESM2_ESM.docx (169 kb)
Fig. SD2 Regression coefficient vectors for the PLS models built for the prediction of TAC and TPC. (DOCX 169 kb)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Tiago A. Catelani
    • 1
  • Ricardo N. M. J. Páscoa
    • 2
  • João Rodrigo Santos
    • 3
  • Leonardo Pezza
    • 1
  • Helena R. Pezza
    • 1
  • José L. F. C. Lima
    • 2
  • João A. Lopes
    • 4
  1. 1.Institute of ChemistryState University of São Paulo - UNESPAraraquaraBrazil
  2. 2.REQUIMTE/LAQV - Departamento de Ciências Químicas, Laboratório de Química Aplicada, Faculdade de FarmáciaUniversidade do PortoPortoPortugal
  3. 3.REQUIMTE/LAQV - Departamento de Química e Bioquímica, Faculdade de CiênciasUniversidade do PortoPortoPortugal
  4. 4.Research Institute for Medicines (iMed.ULisboa), Faculdade de FarmáciaUniversidade de LisboaLisbonPortugal

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