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Characterization of Polyphenol Oxidase Activity in Juices from 12 Underutilized Tropical Fruits with High Agroindustrial Potential

Abstract

Polyphenol oxidase activities of lulo, mangosteen, Castilla blackberry, mango, apple guava, tree tomato, papaya, soursop, banana passionfruit, giant granadilla, sweet granadilla, and passion fruit were determined and related to their physicochemical properties. Mangosteen and lulo showed the highest activities (0.1435 and 0.1298 U mL−1, respectively), while passion fruit and banana passionfruit had the lowest ones (0.0101 and 0.0093 U mL−1). The two fruits with highest PPO activity were also the ones in which highest color differences (ΔE) during the first 90 min after fruit crushing were achieved. The Principal Component Analysis revealed a high correlation between PPO activity and total phenolic content for the compounds that are susceptible to act as enzyme substrate. These two variables were also highly correlated with the decrease produced in brightness (measured with the CIELab parameter L*).

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References

  1. Arogba, S. S., Ajiboye, O. L., Ugboko, L. A., Essienette, S. Y., & Afolabi, P. O. (1998). Properties of polyphenol oxidase in mango (Mangifera indica) kernel. Journal of the Science of Food and Agriculture, 77, 459–462.

    Article  CAS  Google Scholar 

  2. Arvanitoyannis, I. S., & Vlachos, A. (2007). Implementation of physicochemical and sensory analysis in conjunction with multivariate analysis towards assessing olive oil Authentication/Adulteration. Critical Reviews in Food Science and Nutrition, 47(5), 441–498.

    Article  CAS  Google Scholar 

  3. Arvanitoyannis, I. S., Chalhoub, C., Gotsiou, P., Lydakis-Simantiris, N., & Kefalas, P. (2005). Novel quality control methods in conjunction with chemometrics (multivariate analysis) for detecting honey authenticity. Critical Reviews in Food Science and Nutrition, 45(3), 193–203.

    Article  CAS  Google Scholar 

  4. Aular, J., Rodríguez, Y., Roa, S., Iade, P., & Antolínez, M. (2004). Características del fruto de cuatro pasifloras de la zona andina venezolana. Bioagro, 16(2), 137–142.

    Google Scholar 

  5. Bora, P. S., Holschuh, H. J., & da Silva Vasconcelos, M. A. (2004). Characterization of polyphenol oxidase of soursop (Annona muricata L.) fruit and a comparative study of its inhibition in enzyme extract and in pulp. Ciencia y Tecnologia Alimentaria, 4(4), 267–273.

    Article  CAS  Google Scholar 

  6. Camo, A. S. (1996). The unscrambler 6. Trondheim (Norway): User’s Guide.

    Google Scholar 

  7. Cano, M. P., Lobo, M. G., de Ancos, B., & Galeazzi, M. A. M. (1996). Polyphenol oxidase from Spanish hermaphrodite and female papaya fruits (Carica papaya sv. Sunrise, Solo group). Journal of Agricultural and Fruit Chemistry, 44, 3075–3079.

    Article  CAS  Google Scholar 

  8. Constabel, C. P., Lynn, Y., Patton, J. J., & Christopher, M. E. (2000). Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory. Plant Physiology, 124, 285–295.

    Article  CAS  Google Scholar 

  9. Falguera, V., Pagán, J., & Ibarz, A. (2010a). A kinetic model describing melanin formation by means of mushroom tyrosinase. Food Research International, 43(1), 66–69.

    Article  CAS  Google Scholar 

  10. Falguera, V., Gatius, F., Pagán, J., & Ibarz, A. (2010b). Kinetic analysis of melanogenesis by means of Agaricus bisporus tyrosinase. Food Research International, 43(3), 1174–1179.

    Article  CAS  Google Scholar 

  11. Falguera, V., Pagán, J., & Ibarz, A. (2011). Effect of UV irradiation on enzymatic activities and physicochemical properties of apple juices from different varieties. LWT Food Science and Technology, 44, 115–119.

    Article  CAS  Google Scholar 

  12. Gancel, A. L., Alter, P., Dhuique-Mayer, C., Ruales, J., & Vaillant, F. (2008). Identifying carotenoids and phenolic compounds in naranjilla (Solanum quitoense Lam. Var. Puyo Hybrid), an Andean fruit. Journal of Agricultural and Food Chemistry, 56, 11890–11899.

    Article  CAS  Google Scholar 

  13. García-Molina, F., Muñoz, J. L., Varón, R., Rodríguez-López, J. N., García-Cánovas, F., & Tudela, J. (2007). A review on spectrophotometric methods for measuring the monophenolase and diphenolase activities of tyrosinase. Journal of Agricultural and Food Chemistry, 55, 9739–9749.

    Article  Google Scholar 

  14. IFFJP (1972, 1984, 1996). International federation of fruit juice producers. analysen-methoden. Zug, Switzerland: Fruit-Union Suisse.

  15. Jiang, Y., Duan, X., Joyce, D., Zhang, Z., & Li, J. (2004). Advances in understanding of enzymatic browning in harvested litchi fruit. Food Chemistry, 88, 443–446.

    Article  CAS  Google Scholar 

  16. Ladaniya, M. (2008). Citrus fruit. Biology, technology and evaluation. San Diego (USA): Elsevier.

    Google Scholar 

  17. Li, L., & Steffens, C. (2002). Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta, 215, 239–247.

    Article  CAS  Google Scholar 

  18. Lozano, J. E., Drudis-Biscarri, R., & Ibarz-Ribas, A. (1994). Enzymatic browning in apple pulps. Journal of Food Science, 59(3), 564–567.

    Article  CAS  Google Scholar 

  19. Mayer, A. M., & Harel, E. (1979). Polyphenol oxidases in plants. Phytochemistry, 18, 193–215.

    Article  CAS  Google Scholar 

  20. Mertz, C., Gancel, A. L., Gunata, Z., Alter, P., Dhuique-Mayer, C., Vaillant, F., et al. (2009). Phenolic compounds, carotenoids and antioxidant activity of three tropical fruits. Journal of Food Composition and Analysis, 22, 381–387.

    Article  CAS  Google Scholar 

  21. Ngawhirunpat, T., Opanasopi, P., Sukma, M., Sittisombut, C., Kat, A., & Adachi, I. (2010). Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana). Pharmaceutical Biology, 48(1), 55–62.

    Article  CAS  Google Scholar 

  22. Queiroz, C., Mendes-Lopes, M. L., Fialho, E., & Valente-Mesquita, V. L. (2008). Polyphenol oxidase: characteristics and mechanisms of browning control. Food Reviews International, 24, 361–375.

    Article  CAS  Google Scholar 

  23. Rojas-Barquera, D., & Narváez-Cuenca, C. E. (2009). Determinación de vitamina C, compuestos fenólicos totales y actividad antioxidante de frutas de guayaba (Psidium guajava L.) cultivadas en Colombia. Quimica Nova, 32(9), 2336–2340.

    Article  CAS  Google Scholar 

  24. Shaghaghi, M., Manzoori, J. L., & Jouybanb, A. (2008). Determination of total phenols in tea infusions, tomato and apple juice by terbium sensitized fluorescence method as an alternative approach to the Folin–Ciocalteu spectrophotometric method. Food Chemistry, 108, 695–701.

    Article  CAS  Google Scholar 

  25. Tzouros, N. E., & Arvanitoyannis, I. S. (2001). Agricultural produces: synopsis of employed quality control methods for the authentication of foods and for the classification of foods according to their variety of geographical origin. Critical Reviews in Food Science and Nutrition, 41(4), 287–319.

    Article  CAS  Google Scholar 

  26. Ülker-Yerlitürk, F., Arslan, O., Sinan, S., Gencer, N., & Özensoy, O. (2008). Characterization of Polyphenol oxidase from wild pear (Pyrus elaegrifolia). Journal of Food Biochemistry, 32, 368–383.

    Article  Google Scholar 

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Acknowledgment

V. Falguera: The research leading to this work has been partially supported by the Programa de Formación del Profesorado Universitario from the Ministerio de Educación y Ciencia of the Spanish Government.

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Correspondence to Víctor Falguera.

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Falguera, V., Sánchez-Riaño, A.M., Quintero-Cerón, J.P. et al. Characterization of Polyphenol Oxidase Activity in Juices from 12 Underutilized Tropical Fruits with High Agroindustrial Potential. Food Bioprocess Technol 5, 2921–2927 (2012). https://doi.org/10.1007/s11947-011-0521-y

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Keywords

  • Polyphenol oxidase
  • Enzymatic browning
  • Tropical fruits
  • Fruit characterization
  • Multivariate analysis