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Single-Cell RNA-Sequencing in Glioma

  • Neuro-oncology (S Nagpal, Section Editor)
  • Published:
Current Oncology Reports Aims and scope Submit manuscript

Abstract

Purpose of Review

In this review, we seek to summarize the literature concerning the use of single-cell RNA-sequencing for CNS gliomas.

Recent Findings

Single-cell analysis has revealed complex tumor heterogeneity, subpopulations of proliferating stem-like cells and expanded our view of tumor microenvironment influence in the disease process.

Summary

Although bulk RNA-sequencing has guided our initial understanding of glioma genetics, this method does not accurately define the heterogeneous subpopulations found within these tumors. Single-cell techniques have appealing applications in cancer research, as diverse cell types and the tumor microenvironment have important implications in therapy. High cost and difficult protocols prevent widespread use of single-cell RNA-sequencing; however, continued innovation will improve accessibility and expand our of knowledge gliomas.

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Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

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Authors and Affiliations

  1. Stanford University School of Medicine, Stanford, CA, USA

    Eli Johnson, Katherine L. Dickerson & Ian D. Connolly

  2. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA

    Melanie Hayden Gephart

  3. Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA, 94305, USA

    Melanie Hayden Gephart

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  1. Eli Johnson
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  2. Katherine L. Dickerson
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  4. Melanie Hayden Gephart
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Correspondence to Melanie Hayden Gephart.

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Eli Johnson, Katherine L. Dickerson, Ian D. Connolly, and Melanie Hayden Gephart declare they have no conflict of interest.

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This article does not contain any studies with human or animal subjects performed by any of the authors.

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This article is part of the Topical Collection on Neuro-oncology

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Johnson, E., Dickerson, K.L., Connolly, I.D. et al. Single-Cell RNA-Sequencing in Glioma. Curr Oncol Rep 20, 42 (2018). https://doi.org/10.1007/s11912-018-0673-2

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  • DOI: https://doi.org/10.1007/s11912-018-0673-2

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