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Novel Imaging Methods for Analysis of Tissue Resident Cells in HIV/SIV

  • HIV Pathogenesis and Treatment (AL Landay, Section Editor)
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Abstract

The use of advanced tissue-imaging methodologies has greatly facilitated the study of molecular mechanisms and cellular interactions in humans and animal models of disease. Particularly, in HIV research, there is an ever-increasing demand for a comprehensive analysis of immune cell dynamics at tissue level stemming from the need to advance our understanding of those interactions that regulate the generation of adaptive antigen-specific immune responses. The latter is critical for the development of vaccines to elicit broadly neutralizing antibodies as well as for the discovery of novel targets for immuno-therapies to strengthen the cytolytic arm of the immune system at local level. In this review, we focus on current and emerging imaging technologies, discuss their strengths and limitations, and examine how such technologies can inform the development of new treatments and vaccination strategies. We also present some perspective on the future of the technology development.

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Acknowledgments

This research was supported by the Intramural Research Program of the Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health.

The authors would like to acknowledge the Miami Center for AIDS Research (CFAR) Laboratory Core at the University of Miami for partially supporting under award number P30AI073961.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, 33136-1013, USA

    Eirini Moysi

  2. AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA

    Jacob D. Estes

  3. Immunology Laboratory, Vaccine Research Center, NIAID, National Institutes of Health, Building 40, 40 Convent Drive, Bethesda, MD, 20892-3005, USA

    Constantinos Petrovas

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  1. Eirini Moysi
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  2. Jacob D. Estes
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Correspondence to Constantinos Petrovas.

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This article is part of the Topical Collection on HIV Pathogenesis and Treatment

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Moysi, E., Estes, J.D. & Petrovas, C. Novel Imaging Methods for Analysis of Tissue Resident Cells in HIV/SIV. Curr HIV/AIDS Rep 13, 38–43 (2016). https://doi.org/10.1007/s11904-016-0300-5

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