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How In Vitro Assays Contribute to Allergy Diagnosis

  • Immunologic/Diagnostic Tests in Allergy (M Chapman and A Pomés, Section Editors)
  • Published:
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Abstract

Diagnosis of allergic disorders is based upon the clinical history of the disease, the immunoglobulin E (IgE) antibody response, and the allergen exposure. During the last decade, many changes have occurred in the in vitro diagnostic tests used in daily practice. The most important one is the use of allergenic molecules, which helps to define severe profile of allergy and/or to better understand cross-reactivity. The correlation between IgE sensitization and bronchial or nasal response in provocation tests is not so clear, which implies that such tests are still helpful in allergy diagnosis. In order to strengthen the link between a real allergen exposure and allergic symptoms, environmental allergen load assessment can be performed. For clinicians, it appears obvious to know the pollen count to treat their patients; however, they rarely measure the allergen load in the indoor environment, while nowadays home-tests (semi-quantitative or quantitative) make the assessment very easy. In the future, assessment of the environmental exposure (preferably with an indoor technician) of an allergic patient should take into account not only the allergens but also the other indoor pollutants, which could enhance respiratory symptoms in allergic patients.

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References

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  1. Thomas WR, Stewart GA, Simpson RJ, Chua KY, Plozza TM, Dilworth RJ, et al. Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli. Int Arch Allergy Appl Immunol. 1988;85(1):127–9.

    Article  CAS  PubMed  Google Scholar 

  2. Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, et al. Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB J. 2002;16(3):414–6.

    CAS  PubMed  Google Scholar 

  3. Chapman MD, Ferreira F, Villalba M, Cromwell O, Bryan D, Becker WM, et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122(5):882–9.

    Article  PubMed  Google Scholar 

  4. Bernstein IL, Li JT, Bernstein DI, Hamilton R, Spector SL, Tan R, et al. Allergy diagnostic testing: an updated practice parameter. Ann Allergy Asthma Immunol. 2008;100(3Suppl 3):S1–148.

    CAS  Google Scholar 

  5. Hoffmann HJ, Santos AF, Mayorga C, Nopp A, Eberlein B, Ferrer M, et al. The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy.

  6. Gomez E, Campo P, Rondon C, Barrionuevo E, Blanca-Lopez N, Torres MJ, et al. Role of the basophil activation test in the diagnosis of local allergic rhinitis. J Allergy Clin Immunol. 2013;132(4):975–6.

    Article  PubMed  Google Scholar 

  7. Rondon C, Campo P, Togias A, Fokkens WJ, Durham SR, Powe DG, et al. Local allergic rhinitis: concept, pathophysiology, and management. J Allergy Clin Immunol. 2012;129(6):1460–7.

    Article  CAS  PubMed  Google Scholar 

  8. Meyer CH, Bond JF, Chen MS, Kasaian MT. Comparison of the levels of the major allergens Der p I and Der p II in standardized extracts of the house dust mite, Dermatophagoides pteronyssinus. Clin Exp Allergy. 1994;24(11):1041–8.

    Article  CAS  PubMed  Google Scholar 

  9. Curin M, Reininger R, Swoboda I, Focke M, Valenta R, Spitzauer S. Skin prick test extracts for dog allergy diagnosis show considerable variations regarding the content of major and minor dog allergens. Int Arch Allergy Immunol. 2011;154(3):258–63.

    Article  PubMed  Google Scholar 

  10. Thomsen GF, Schlunssen V, Skadhauge LR, Malling TH, Sherson DL, Omland O, et al. Are allergen batch differences and the use of double skin prick test important? BMC Pulm Med. 2015;15:33.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Casset A, Mari A, Purohit A, Resch Y, Weghofer M, Ferrara R, et al. Varying allergen composition and content affects the in vivo allergenic activity of commercial Dermatophagoides pteronyssinus extracts. Int Arch Allergy Immunol. 2012;159(3):253–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Kespohl S, Maryska S, Zahradnik E, Sander I, Bruning T, Raulf-Heimsoth M. Biochemical and immunological analysis of mould skin prick test solution: current status of standardization. Clin Exp Allergy. 2013;43(11):1286–96.

    Article  CAS  PubMed  Google Scholar 

  13. Gabriel MF, Tavares-Ratado P, Peixinho CM, Romeira AM, Taborda-Barata L, Postigo I, et al. Evaluation and comparison of commercially available latex extracts for skin prick tests. J Investig Allergol Clin Immunol. 2013;23(7):478–86.

    CAS  PubMed  Google Scholar 

  14. Focke M, Marth K, Flicker S, Valenta R. Heterogeneity of commercial timothy grass pollen extracts. Clin Exp Allergy. 2008;38(8):1400–8.

    Article  CAS  PubMed  Google Scholar 

  15. Focke M, Marth K, Valenta R. Molecular composition and biological activity of commercial birch pollen allergen extracts. Eur J Clin Invest. 2009;39(5):429–36.

    Article  CAS  PubMed  Google Scholar 

  16. Patterson ML, Slater JE. Characterization and comparison of commercially available German and American cockroach allergen extracts. Clin Exp Allergy. 2002;32(5):721–7.

    Article  CAS  PubMed  Google Scholar 

  17. Moingeon P, Batard T, Nony E, Hrabina M, Frati F. Quality control of house dust mite extracts for allergen immunotherapy. Int Arch Allergy Immunol. 2013;161(3):285–6.

    Article  PubMed  Google Scholar 

  18. de Blay F, Doyen V, Bloch-Morot E, Caillot D, Gayraud J, de Laval A, et al. French application of the European guidelines for regulation of allergenic extracts. J Allergy Clin Immunol. 2013;131(5):1435–7.

    Article  PubMed  Google Scholar 

  19. Valenta R, Lidholm J, Niederberger V, Hayek B, Kraft D, Gronlund H. The recombinant allergen-based concept of component-resolved diagnostics and immunotherapy (CRD and CRIT). Clin Exp Allergy. 1999;29(7):896–904.

    Article  CAS  PubMed  Google Scholar 

  20. Jakob T, Forstenlechner P, Matricardi P, Kleine-Tebbe J. Molecular allergy diagnostics using multiplex assays: methodological and practical considerations for use in research and clinical routine: part 21 of the Series Molecular Allergology. Allergo J Int. 2015;24:320–32.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Wohrl S, Vigl K, Zehetmayer S, Hiller R, Jarisch R, Prinz M, et al. The performance of a component-based allergen-microarray in clinical practice. Allergy. 2006;61(5):633–9.

    Article  CAS  PubMed  Google Scholar 

  22. Bousquet J, Chanez P, Chanal I, Michel FB. Comparison between RAST and Pharmacia CAP system: a new automated specific IgE assay. J Allergy Clin Immunol. 1990;85(6):1039–43.

    Article  CAS  PubMed  Google Scholar 

  23. Stringari G, Tripodi S, Caffarelli C, Dondi A, Asero R, Di Rienzo Businco A, et al. The effect of component-resolved diagnosis on specific immunotherapy prescription in children with hay fever. J Allergy Clin Immunol. 2014;134(1):75–81.

    Article  PubMed  Google Scholar 

  24. Scala G, Miceli Sopo S. When are serum specific IgE levels positive? J Allergy Clin Immunol. 2015;135(1):291–2.

    Article  PubMed  Google Scholar 

  25. Tripodi S, Caffarelli C, Stringari G, Dondi A, Asero R, Matricardi PM. Reply: To J Allergy Clin Immunol. 2015;135(1):292–3.

  26. Hamilton RG. Clinical laboratories worldwide need to report IgE antibody results on clinical specimens as analytical results and not use differential positive thresholds. J Allergy Clin Immunol. 2015;136(3):811–2.

    Article  PubMed  Google Scholar 

  27. de Blay F, Zana H, Offner M, Verot A, Velten M, Pauli G. Receiver operating characteristic analysis: a useful method for a comparison of the clinical relevance of two in vitro IgE tests. J Allergy Clin Immunol. 1993;92(2):255–63.

    Article  PubMed  Google Scholar 

  28. Bakke PS. Designing studies for diagnostic tests. Clin Respir J. 2008;2 Suppl 1:72–5.

    Article  PubMed  Google Scholar 

  29. Linden CC, Misiak RT, Wegienka G, Havstad S, Ownby DR, Johnson CC, et al. Analysis of allergen specific IgE cut points to cat and dog in the Childhood Allergy Study. Ann Allergy Asthma Immunol. 2011;106(2):153–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Matricardi PM, Nisini R, Biselli R, D’Amelio R. Evaluation of the overall degree of sensitization to airborne allergens by a single serologic test: implications for epidemiologic studies of allergy. J Allergy Clin Immunol. 1994;93(1 Pt 1):68–79.

    Article  CAS  PubMed  Google Scholar 

  31. Matricardi PM, Kleine-Tebbe J, Hoffmann HJ, Valenta R, Hilger C, Hofmaier S, et al. EAACI Molecular allergology user’s guide. Pediatr Allergy Immunol. 2016;27 Suppl 23:1–250. The up-to-date and essentiel guide on molecular allergology for allergists, which provides comprehensive information on important allergens and describes the diagnostic options using component-resolved diagnostics.

    Article  CAS  PubMed  Google Scholar 

  32. Minami T, Fukutomi Y, Lidholm J, Yasueda H, Saito A, Sekiya K, et al. IgE Abs to Der p 1 and Der p 2 as diagnostic markers of house dust mite allergy as defined by a bronchoprovocation test. Allergol Int. 2015;64(1):90–5.

    Article  CAS  PubMed  Google Scholar 

  33. Huss-Marp J, Gutermuth J, Schaffner I, Darsow U, Pfab F, Brockow K, et al. Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis. Allergo J Int. 2015;24:46–53.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Ahlgrim C, Gutermuth J, Onell A, Borres MP, Schaffner I, Darsow U, et al. Comparison of molecular multiplex and singleplex analysis of IgE to grass pollen allergens in untreated German grass pollen-allergic patients. J Investig Allergol Clin Immunol. 2015;25(3):190–5. This study provides a detailed comparison, on molecular level, between multiplex and singleplex analysis of IgE against grass pollen allergens.

    CAS  PubMed  Google Scholar 

  35. Lupinek C, Wollmann E, Baar A, Banerjee S, Breiteneder H, Broecker BM, et al. Advances in allergen-microarray technology for diagnosis and monitoring of allergy: the MeDALL allergen-chip. Methods. 2014;66(1):106–19.

    Article  CAS  PubMed  Google Scholar 

  36. Sircar G, Sarkar D, Bhattacharya SG, Saha S. Allergen databases. Methods Mol Biol. 2014;1184:165–81. Up-to-date review on allergen databases useful for clinician seeking information on allergens to interpret the “allergomics” data.

    Article  PubMed  Google Scholar 

  37. Canonica GW, Ansotegui IJ, Pawankar R, Schmid-Grendelmeier P, van Hage M, Baena-Cagnani CE, et al. A WAO - ARIA - GA(2)LEN consensus document on molecular-based allergy diagnostics. World Allergy Organ J. 2013;6(1):17.

    Article  PubMed  PubMed Central  Google Scholar 

  38. Bryant DH, Burns MW, Lazarus L. The correlation between skin tests, bronchial provocation tests and the serum level of IgE specific for common allergens in patients with asthma. Clin Allergy. 1975;5(2):145–57.

    Article  CAS  PubMed  Google Scholar 

  39. Barnig C, Purohit A, Casset A, Sohy C, Lieutier-Colas F, Sauleau E, et al. Nonallergic airway hyperresponsiveness and allergen-specific IgE levels are the main determinants of the early and late asthmatic response to allergen. J Investig Allergol Clin Immunol. 2013;23(4):267–74.

    CAS  PubMed  Google Scholar 

  40. Bousquet J, Lebel B, Dhivert H, Bataille Y, Martinot B, Michel FB. Nasal challenge with pollen grains, skin-prick tests and specific IgE in patients with grass pollen allergy. Clin Allergy. 1987;17(6):529–36.

    Article  CAS  PubMed  Google Scholar 

  41. Huss-Marp J, Darsow U, Brockow K, Pfab F, Weichenmeier I, Schober W, et al. Can immunoglobulin E-measurement replace challenge tests in allergic rhinoconjunctivits to grass pollen? Clin Exp Allergy. 2011;41(8):1116–24.

    Article  CAS  PubMed  Google Scholar 

  42. de Blay F, Doyen V, Lutz C, Godet J, Barnig C, Qi S, et al. A new, faster, and safe nasal provocation test method for diagnosing mite allergic rhinitis. Ann Allergy Asthma Immunol. 2015;115(5):385–90. Useful for clinicians, this study gives information on the rapid nasal provocation test for mite allergic patients.

    Article  PubMed  Google Scholar 

  43. Chen Z, Rihs HP, Slater JE, Paupore EJ, Schneider EM, Baur X. The absence of Hev b 5 in capture antigen may cause false-negative results in serologic assays for latex-specific IgE antibodies. J Allergy Clin Immun. 2000;105(1):S83–S.

    Article  Google Scholar 

  44. Lundberg M, Chen Z, Rihs HP, Wrangsjo K. Recombinant spiked allergen extract. Allergy. 2001;56(8):794–5.

    Article  CAS  PubMed  Google Scholar 

  45. Hamilton RG, Adkinson Jr NF. Diagnosis of natural rubber latex allergy: multicenter latex skin testing efficacy study. Multicenter Latex Skin Testing Study Task Force. J Allergy Clin Immunol. 1998;102(3):482–90.

    Article  CAS  PubMed  Google Scholar 

  46. Huss-Marp J, Raulf M, Jakob T. Spiking with recombinant allergens to improve allergen extracts: benefits and limitations for the use in routine diagnostics: part 19 of the Series Molecular Allergology. Allergo J Int. 2015;24:236–43.

    Article  PubMed  PubMed Central  Google Scholar 

  47. Raulf-Heimsoth M, Rihs HP, Rozynek P, Cremer R, Gaspar A, Pires G, et al. Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis). Clin Exp Allergy. 2007;37(11):1657–67.

    Article  CAS  PubMed  Google Scholar 

  48. Ebo DG, Hagendorens MM, De Knop KJ, Verweij MM, Bridts CH, De Clerck LS, et al. Component-resolved diagnosis from latex allergy by microarray. Clin Exp Allergy. 2010;40(2):348–58.

    Article  CAS  PubMed  Google Scholar 

  49. Sylvestre L, Jegu J, Metz-Favre C, Barnig C, Qi S, de Blay F. Component-based allergen-microarray: Der p 2 and Der f 2 dust mite sensitization is more common in patients with severe asthma. J Investig Allergol Clin Immunol. 2016;26(2):141–3. Study showing the interest of Der p 2 IgE measurement in asthmatic allergic patients sensitized to mite.

    Article  CAS  PubMed  Google Scholar 

  50. Batard T, Baron-Bodo V, Martelet A, Le Mignon M, Lemoine P, Jain K, et al. Patterns of IgE sensitization in house dust mite-allergic patients: implications for allergen immunotherapy. Allergy. 2016;71(2):220–9.

    Article  CAS  PubMed  Google Scholar 

  51. Uriarte SA, Sastre J. Clinical relevance of molecular diagnosis in pet allergy. Allergy. 2016;71(7):1066–8. This study reports pet albumines as marker for pet allergic patients suffering from severe asthma.

    Article  CAS  PubMed  Google Scholar 

  52. Bjerg A, Winberg A, Berthold M, Mattsson L, Borres MP, Ronmark E. A population-based study of animal component sensitization, asthma, and rhinitis in schoolchildren. Pediatr Allergy Immunol. 2015;26(6):557–63.

    Article  PubMed  Google Scholar 

  53. Konradsen JR, Nordlund B, Onell A, Borres MP, Gronlund H, Hedlin G. Severe childhood asthma and allergy to furry animals: refined assessment using molecular-based allergy diagnostics. Pediatr Allergy Immunol. 2014;25(2):187–92. This study describes the benefit of molecular allergology diagnostics to identify patients with pet allergy with higher risk of symptom severity.

    Article  PubMed  Google Scholar 

  54. Melioli G, Bonifazi F, Bonini S, Maggi E, Mussap M, Passalacqua G, et al. The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms. Clin Biochem. 2011;44(12):1005–11.

    Article  PubMed  Google Scholar 

  55. Garcia BE, Martinez-Aranguren R, Bernard Alonso A, Gamboa P, Feo Brito F, Bartra J, et al. Is the ISAC 112 microarray useful in the diagnosis of pollinosis in Spain? J Investig Allergol Clin Immunol. 2016;26(2):92–9.

    Article  CAS  PubMed  Google Scholar 

  56. Asero R, Jimeno L, Barber D. Preliminary results of a skin prick test-based study of the prevalence and clinical impact of hypersensitivity to pollen panallergens (polcalcin and profilin). J Investig Allergol Clin Immunol. 2010;20(1):35–8.

    CAS  PubMed  Google Scholar 

  57. Alvarado MI, Jimeno L, De La Torre F, Boissy P, Rivas B, Lazaro MJ, et al. Profilin as a severe food allergen in allergic patients overexposed to grass pollen. Allergy. 2014;69(12):1610–6. This study reports that profilins are severe food allergens in food-allergic patients overexposed to grass pollen. Important interest for oral provocation of food allergic patients in areas where grass pollen allergy is dominant.

    Article  CAS  PubMed  Google Scholar 

  58. McKenna OE, Asam C, Araujo GR, Roulias A, Goulart LR, Ferreira F. How relevant is panallergen sensitization in the development of allergies? Pediatr Allergy Immunol. 2016;27(6):560–8. An up-to-date review on the clinical relevance of panallergens.

    Article  PubMed  PubMed Central  Google Scholar 

  59. Burbach GJ, Heinzerling LM, Edenharter G, Bachert C, Bindslev-Jensen C, Bonini S, et al. GA(2)LEN skin test study II: clinical relevance of inhalant allergen sensitizations in Europe. Allergy. 2009;64(10):1507–15.

    Article  CAS  PubMed  Google Scholar 

  60. Thibaudon M, Caillaud D, Besancenot JP. Methods of studying airborne pollen and pollen calendars. Rev Mal Respir. 2013;30(6):463–79.

    Article  CAS  PubMed  Google Scholar 

  61. Buters J, Galan C, Thibaudon M. The Hialine project: allergen release from pollen across 10 european countries. Proccedings del 9th International Congress on Aerobiology. 2010;Buenos Aires:37–8.

  62. De Linares C, Diaz de la Guardia C, Nieto Lugilde D, Alba F. Airborne study of grass allergen (Lol p 1) in different-sized particles. Int Arch Allergy Immunol. 2010;152(1):49–57.

    Article  PubMed  Google Scholar 

  63. Buters JTM, Thibaudon M, Smith M, Kennedy R, Rantio-Lehtimaki A, Albertini R, et al. Release of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE study. Atmos Environ. 2012;55:496–505. An important paper on the first measurements of pollen allergens.

    Article  CAS  Google Scholar 

  64. GINA. Global initiative for asthma. http://ginasthma.org/. 2016.

  65. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol. 1997;100(6 Pt 1):S2–24.

    Article  CAS  PubMed  Google Scholar 

  66. Pauli G, Quoix E, Hedelin G, Bessot JC, Ott M, Dietemann A. Mite allergen content in mattress dust of Dermatophagoides-allergic asthmatics/rhinitics and matched controls. Clin Exp Allergy. 1993;23(7):606–11.

    Article  CAS  PubMed  Google Scholar 

  67. Munir AK, Einarsson R, Schou C, Dreborg SK. Allergens in school dust. I. The amount of the major cat (Fel d I) and dog (Can f I) allergens in dust from Swedish schools is high enough to probably cause perennial symptoms in most children with asthma who are sensitized to cat and dog. J Allergy Clin Immunol. 1993;91(5):1067–74.

    Article  CAS  PubMed  Google Scholar 

  68. de Andrade AD, Charpin D, Birnbaum J, Lanteaume A, Chapman M, Vervloet D. Indoor allergen levels in day nurseries. J Allergy Clin Immunol. 1995;95(6):1158–63.

    Article  PubMed  Google Scholar 

  69. Patchett K, Lewis S, Crane J, Fitzharris P. Cat allergen (Fel d 1) levels on school children’s clothing and in primary school classrooms in Wellington. New Zealand J Allergy Clin Immunol. 1997;100(6 Pt 1):755–9.

    Article  CAS  PubMed  Google Scholar 

  70. Sarpong SB, Wood RA, Karrison T, Eggleston PA. Cockroach allergen (Bla g 1) in school dust. J Allergy Clin Immunol. 1997;99(4):486–92.

    Article  CAS  PubMed  Google Scholar 

  71. Custovic A, Green R, Taggart SC, Smith A, Pickering CA, Chapman MD, et al. Domestic allergens in public places. II: Dog (Can f1) and cockroach (Bla g 2) allergens in dust and mite, cat, dog and cockroach allergens in the air in public buildings. Clin Exp Allergy. 1996;26(11):1246–52.

    Article  CAS  PubMed  Google Scholar 

  72. Chapman MD, Tsay A, Vailes LD. Home allergen monitoring and control-improving clinical practice and patient benefits. Allergy. 2001;56(7):604–10.

    Article  CAS  PubMed  Google Scholar 

  73. Casset A, Marchand C, Purohit A, le Calve S, Uring-Lambert B, Donnay C, et al. Inhaled formaldehyde exposure: effect on bronchial response to mite allergen in sensitized asthma patients. Allergy. 2006;61(11):1344–50.

    Article  CAS  PubMed  Google Scholar 

  74. Roda C, Guihenneuc-Jouyaux C, Momas I. Environmental triggers of nocturnal dry cough in infancy: new insights about chronic domestic exposure to formaldehyde in the PARIS birth cohort. Environ Res. 2013;123:46–51.

    Article  CAS  PubMed  Google Scholar 

  75. Portnoy J, Chew GL, Phipatanakul W, Williams PB, Grimes C, Kennedy K, et al. Environmental assessment and exposure reduction of cockroaches: a practice parameter. J Allergy Clin Immunol. 2013;132(4):802–8.

    Article  PubMed  Google Scholar 

  76. Portnoy J, Miller JD, Williams PB, Chew GL, Zaitoun F, Phipatanakul W, et al. Environmental assessment and exposure control of dust mites: a practice parameter. Ann Allergy Asthma Immunol. 2013;111(6):465–507.

    Article  PubMed  Google Scholar 

  77. Phipatanakul W, Matsui E, Portnoy J, Williams PB, Barnes C, Kennedy K, et al. Environmental assessment and exposure reduction of rodents: a practice parameter. Ann Allergy Asthma Immunol. 2012;109(6):375–87.

    Article  PubMed  PubMed Central  Google Scholar 

  78. Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods. 1989;118(2):227–35.

    Article  CAS  PubMed  Google Scholar 

  79. Pate AD, Hamilton RG, Ashley PJ, Zeldin DC, Halsey JF. Proficiency testing of allergen measurements in residential dust. J Allergy Clin Immunol. 2005;116(4):844–50.

    Article  CAS  PubMed  Google Scholar 

  80. van Ree R. The CREATE project: EU support for the improvement of allergen standardization in Europe. Allergy. 2004;59(6):571–4.

    Article  PubMed  Google Scholar 

  81. Vieths S, Barber D, Chapman M, Costanzo A, Daas A, Fiebig H, et al. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. reference standards and validation of ELISA methods for their measurement. Results from feasibility studies. Pharmeur Bio Sci Notes. 2012;2012:118–34.

    CAS  PubMed  Google Scholar 

  82. Filep S, Tsay A, Vailes L, Gadermaier G, Ferreira F, Matsui E, et al. A multi-allergen standard for the calibration of immunoassays: CREATE principles applied to eight purified allergens. Allergy. 2012;67(2):235–41.

    Article  CAS  PubMed  Google Scholar 

  83. Matsui EC, Simons E, Rand C, Butz A, Buckley TJ, Breysse P, et al. Airborne mouse allergen in the homes of inner-city children with asthma. J Allergy Clin Immunol. 2005;115(2):358–63.

    Article  CAS  PubMed  Google Scholar 

  84. Muti D, Purohit A, Dazy A, Verot A, de Blay F. Mouse (Mus m1) and rat (Rat n1) allergen levels in dust from private and public houses in Strasbourg, France are lower than houses in the U.S.A. Eur Ann Allergy Clin Immunol. 2012;44(2):93–5.

    CAS  PubMed  Google Scholar 

  85. Quoix E, Le Mao J, Hoyet C, Pauli G. Prediction of mite allergen levels by guanine measurements in house-dust samples. Allergy. 1993;48(5):306–9.

    Article  CAS  PubMed  Google Scholar 

  86. Haouichat H, Pauli G, Ott M, Hedelin G, de Blay F, Verot A, et al. Reevaluation of the Acarex test ten years later. Rev Fr Allergol. 2000;40(8):783–92.

    Google Scholar 

  87. Mistrello G, Gentili M, Roncarolo D, Antoniotti P, Ottoboni F, Falagiani P. Dot immunobinding assay for detection of mite antigens in house-dust samples. J Med Entomol. 1998;35(2):143–7.

    Article  CAS  PubMed  Google Scholar 

  88. Tsay A, Williams L, Mitchell EB, Chapman MD. A rapid test for detection of mite allergens in homes. Clin Exp Allergy. 2002;32(11):1596–601.

    Article  CAS  PubMed  Google Scholar 

  89. Guyton S, Tsay A, Chapman M, Eggleston P. Performance evaluation of a rapid test for dust mite allergen detection in the home. J Allergy Clin Immunol. 2000;105(1):S289–S.

    Article  Google Scholar 

  90. Saijo Y, Nakagi Y, Sugioka Y, Ito T, Endo H, Kuroda H, et al. Comparative study of simple semiquantitative dust mite allergen tests. Environ Health Prev Med. 2007;12(5):187–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  91. Earle CD, King EM, Tsay A, Pittman K, Saric B, Vailes L, et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol. 2007;119(2):428–33.

    Article  CAS  PubMed  Google Scholar 

  92. King EM, Filep S, Smith B, Platts-Mills T, Hamilton RG, Schmechel D, et al. A multi-center ring trial of allergen analysis using fluorescent multiplex array technology. J Immunol Methods. 2013;387(1–2):89–95.

    Article  CAS  PubMed  Google Scholar 

  93. de Blay F, Heymann PW, Chapman MD, Platts-Mills TA. Airborne dust mite allergens: comparison of group II allergens with group I mite allergen and cat-allergen Fel d I. J Allergy Clin Immunol. 1991;88(6):919–26.

    Article  PubMed  Google Scholar 

  94. Custovic A, Simpson A, Pahdi H, Green RM, Chapman MD, Woodcock A. Distribution, aerodynamic characteristics, and removal of the major cat allergen Fel d 1 in British homes. Thorax. 1998;53(1):33–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  95. Bollinger ME, Wood RA, Chen P, Eggleston PA. Measurement of cat allergen levels in the home by use of an amplified ELISA. J Allergy Clin Immunol. 1998;101(1 Pt 1):124–5.

    Article  CAS  PubMed  Google Scholar 

  96. de Blay F, Sanchez J, Hedelin G, Perez-Infante A, Verot A, Chapman M, et al. Dust and airborne exposure to allergens derived from cockroach (Blattella germanica) in low-cost public housing in Strasbourg (France). J Allergy Clin Immunol. 1997;99(1 Pt 1):107–12.

    Article  PubMed  Google Scholar 

  97. Tovey E, Lucca SD, Poulos L, O’Meara T. The Halogen assay-a new technique for measuring airborne allergen. Methods Mol Med. 2008;138:227–46.

    Article  CAS  PubMed  Google Scholar 

  98. Renstrom A. Exposure to airborne allergens: a review of sampling methods. J Environ Monit. 2002;4(5):619–22.

    Article  PubMed  Google Scholar 

  99. Renstrom A, Karlsson AS, Tovey E. Nasal air sampling used for the assessment of occupational allergen exposure and the efficacy of respiratory protection. Clin Exp Allergy. 2002;32(12):1769–75.

    Article  CAS  PubMed  Google Scholar 

  100. Tovey ER, Willenborg CM, Crisafulli DA, Rimmer J, Marks GB. Most personal exposure to house dust mite aeroallergen occurs during the day. PLoS One. 2013;8(7), e69900.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  101. Tovey ER, Liu-Brennan D, Garden FL, Oliver BG, Perzanowski MS, Marks GB. Time-based measurement of personal mite allergen bioaerosol exposure over 24 hour periods. PLoS One. 2016;11(5), e0153414. A new method to assess mite exposure.

    Article  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Faculty of Pharmacy, Laboratory of Conception and Application of Bioactive Molecules, CNRS, University of Strasbourg, BP 60024, 67401, Illkirch Cedex, France

    Anne Casset

  2. Chest Diseases Department, Strasbourg University Hospital; Federation of Translational Medicine, University of Strasbourg, BP 426, 67091, Strasbourg, France

    Naji Khayath & Frédéric de Blay

Authors
  1. Anne Casset
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  2. Naji Khayath
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  3. Frédéric de Blay
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Correspondence to Frédéric de Blay.

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A. Casset, N. Khayath and F. de Blay declare that they have no conflict of interest.

Human and Animal Rights and Informed Consent

Concerning the authors’ research cited in this paper, all procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

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This article is part of the Topical Collection on Immunologic/Diagnostic Tests in Allergy

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Casset, A., Khayath, N. & de Blay, F. How In Vitro Assays Contribute to Allergy Diagnosis. Curr Allergy Asthma Rep 16, 82 (2016). https://doi.org/10.1007/s11882-016-0659-9

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  • DOI: https://doi.org/10.1007/s11882-016-0659-9

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