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Mg2+-Triggered and pH-Tuned in vitro Assembly of Trehalose-6-Phosphate Synthase

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Wuhan University Journal of Natural Sciences

Abstract

The enzyme OtsA (trehalose-P synthase) plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering technique, we discovered that OtsA in Arthrobacter strain A3 polymerized in the presence of divalent metal ions (Mg2+ or Ca2+), and the kinetics of the assembly was dependent on their concentrations. We identified potential compounds that can affect the kinetics of the polymerization, particularly, heparin, which acts as a very promising inhibitor of the polymerization. The OtsA assembly turns out to be a very delicate process that is finely regulated by pH. OtsA may be in the polymerized form at physiological pH in vivo, suggesting a more complicated mechanism of the enzyme. These unique properties of OtsA provide novel insights into the molecular mechanism of the biosynthesis of trehalose.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, Gansu, China

    Shanshan Zhang, Fan Yang, Yuping Zhang & Jianxi Xiao

  2. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China

    Shanshan Zhang & Zaiman Liu

  3. School of Stomatology, Lanzhou University, Lanzhou, 730000, Gansu, China

    Fan Yang & Linghui Yu

  4. Key Laboratory of Desert & Desertification, Cold & Arid Regions Environmental & Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China

    Ximing Chen

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  1. Shanshan Zhang
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  2. Fan Yang
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  3. Yuping Zhang
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  5. Linghui Yu
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Correspondence to Ximing Chen or Jianxi Xiao.

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Foundation item: Supported by the National Natural Science Foundation of China (21305056) and the Open Fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics (T151402)

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Zhang, S., Yang, F., Zhang, Y. et al. Mg2+-Triggered and pH-Tuned in vitro Assembly of Trehalose-6-Phosphate Synthase. Wuhan Univ. J. Nat. Sci. 23, 396–402 (2018). https://doi.org/10.1007/s11859-018-1339-5

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  • DOI: https://doi.org/10.1007/s11859-018-1339-5

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