Plant Biotechnology Reports

, Volume 13, Issue 2, pp 131–140 | Cite as

A PeMYB-like gene induced by brassinosteroids regulates cellulose synthesis in tobacco

  • Xilong DuEmail author
  • Shan Liu
  • Chang Ma
  • Mingguo Ma
Original Article


MYB is a well-known key transcriptional regulator involved in plant development and metabolism. In this study, a new MYB gene was isolated from Phyllostachys edulis and named PeMYB-like. A close link between brassinosteroids (BRs) and PeMYB-like gene expression was suggested in this study. Functional characterization of PeMYB-like was elucidated through overexpression of this gene in tobacco. Expression of PeMYB-like in ten independent transgenic tobacco lines was quantified by semi-quantitative RT-PCR. The expression levels NtCESA1 and NtCESA2 in transgenic plants were higher than that in control plants. The transgenic lines also showed a significant increase in cellulose content, suggesting that PeMYB-like may enhance cellulose biosynthesis through the upregulation of genes encoding enzymes involved in cellulose biosynthesis in plant stems. Our study indicated that there may be a close link between PeMYB-like gene expression and cellulose biosynthesis; moreover, BR signaling pathway might be involved in cellulose biosynthesis processes through the regulation of the MYB gene transcription.


MYB Phyllostachys edulis Cellulose Brassinosteroids 



This work was supported by the Fundamental Research Funds for the Central Universities (No. 2015ZCQ-CL-03).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Ambawat S, Sharma P, Yadav NR, Yadav RC (2013) MYB transcription factor genes as regulators for plant responses: an overview. Physiol Mol Biol Plants 19:307–321CrossRefGoogle Scholar
  2. Chai G, Wang Z, Tang X, Yu L, Qi G, Wang D, Yan X, Kong Y, Zhou G (2014) R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time. J Exp Bot 65:4255–4269CrossRefGoogle Scholar
  3. Dai X, Wang Y, Yang A, Zhang W-H (2012) OsMYB2P-1, an R2R3 MYB transcription factor, is involved in the regulation of phosphate-starvation responses and root architecture in rice. Plant Physiol 159:169–183CrossRefGoogle Scholar
  4. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573–581CrossRefGoogle Scholar
  5. Endler A, Kesten C, Schneider R, Zhang Y, Ivakov A, Froehlich A, Funke N, Persson S (2015) A mechanism for sustained cellulose synthesis during salt stress. Cell 162:1353–1364CrossRefGoogle Scholar
  6. Guan C, Liu X, Song X, Wang G, Ji J, Jin C (2014) Overexpression of a peroxiredoxin Q gene, SsPrxQ, in Eustoma grandiflorum Shinn enhances its tolerance to salt and high light intensity. Mol Breed 33:657–667CrossRefGoogle Scholar
  7. Horsch R, Fry J, Hoffmann N, Eichholz D, Rogers D, Fraley R (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231CrossRefGoogle Scholar
  8. Kim WC, Ko JH, Han KH (2012) Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis. Plant Mol Biol 78:489–501CrossRefGoogle Scholar
  9. Kim WC, Ko JH, Kim JY, Kim J, Bae HJ, Han KH (2013) MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis. Plant J 73:26–36CrossRefGoogle Scholar
  10. Kuppusamy KT, Chen AY, Nemhauser JL (2009) Steroids are required for epidermal cell fate establishment in Arabidopsis roots. Proc Natl Acad Sci USA 106:8073–8076CrossRefGoogle Scholar
  11. Li J (2010) Regulation of the nuclear activities of brassinosteroid signaling. Curr Opin Plant Biol 13:540–547CrossRefGoogle Scholar
  12. Li J, Jin H (2007) Regulation of brassinosteroid signaling. Trends Plant Sci 12:37–41CrossRefGoogle Scholar
  13. Li L, Yu X, Thompson A, Guo M, Yoshida S, Asami T, Chory J, Yin Y (2009) Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression. Plant J 58:275–286CrossRefGoogle Scholar
  14. Liu Y, Wei M, Hou C, Lu T, Liu L, Wei H, Cheng Y, Wei Z (2017) Functional characterization of Populus PsnSHN2 in coordinated regulation of secondary wall components in tobacco. Sci Rep 7:42CrossRefGoogle Scholar
  15. McCarthy RL, Zhong R, Ye Z (2009) MYB83 is a direct target of SND1 and acts redundantly with MYB46 in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant Cell Physiol 50:1950–1964CrossRefGoogle Scholar
  16. Peng Z, Zhang C, Zhang Y, Hu T, Mu S, Li X, Gao J (2013) Transcriptome sequencing and analysis of the fast growing shoots of moso bamboo (Phyllostachys edulis). PLoS One 8:e78944CrossRefGoogle Scholar
  17. Sánchez-Rodríguez C, Ketelaar K, Schneider R, Villalobos JA, Somerville CR, Persson S, Wallace IS (2017) BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis in Arabidopsis by phosphorylating cellulose synthase 1. Proc Natl Acad Sci USA 114:3533–3538CrossRefGoogle Scholar
  18. Shen H, He X, Poovaiah CR, Wuddineh WA, Ma J, Mann DGJ, Wang H, Jackson L, Tang Y, Neal Stewart C Jr, Chen F, Dixon RA (2012) Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytol 193:121–136CrossRefGoogle Scholar
  19. Shi Y, Liu X, Li X, Dong W, Grierson D, Yin X, Chen K (2017) SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco. J Integr Agric 16:65–75CrossRefGoogle Scholar
  20. Soltész A, Vágújfalvi A, Rizza F, Kerepesi I, Galiba G, Cattivelli L, Coraggio I, Crosatti C (2012) The rice Osmyb4 gene enhances tolerance to frost and improves germination under unfavourable conditions in transgenic barley plants. J Appl Genet 53:133–143CrossRefGoogle Scholar
  21. Sun Y, Veerabomma S, Abdel-Mageed HA, Fokar M, Asami T, Yoshida S, Allen RD (2005) Brassinosteroid regulates fiber development on cultured cotton ovules. Plant Cell Physiol 46:1384–1391CrossRefGoogle Scholar
  22. Vannini C, Campa M, Iriti M, Genga A, Faoro F, Carravieri S, Rotino GL, Rossoni M, Spinardi A, Bracale M (2007) Evaluation of transgenic tomato plants ectopically expressing the rice Osmyb4 gene. Plant Sci 173:231–239CrossRefGoogle Scholar
  23. Xie L, Yang C, Wang X (2011) Brassinosteroids can regulate cellulose biosynthesis by controlling the expression of CESA genes in Arabidopsis. J Exp Bot 62:4495–4506CrossRefGoogle Scholar
  24. Yang C, Li D, Liu X, Ji C, Hao L, Zhao X, Li X, Chen C, Cheng Z, Zhu L (2014) OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.). BMC Plant Biol 14:158CrossRefGoogle Scholar
  25. Zhong R, Lee C, Zhou J, McCarthy RL, Ye Z-H (2008) A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant Cell 20:2763–2782CrossRefGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Lignocellulosic Chemistry, College of Materials Science and TechnologyBeijing Forestry UniversityBeijingPeople’s Republic of China

Personalised recommendations