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Plant Biotechnology Reports

, Volume 13, Issue 2, pp 131–140 | Cite as

A PeMYB-like gene induced by brassinosteroids regulates cellulose synthesis in tobacco

  • Xilong DuEmail author
  • Shan Liu
  • Chang Ma
  • Mingguo Ma
Original Article
  • 84 Downloads

Abstract

MYB is a well-known key transcriptional regulator involved in plant development and metabolism. In this study, a new MYB gene was isolated from Phyllostachys edulis and named PeMYB-like. A close link between brassinosteroids (BRs) and PeMYB-like gene expression was suggested in this study. Functional characterization of PeMYB-like was elucidated through overexpression of this gene in tobacco. Expression of PeMYB-like in ten independent transgenic tobacco lines was quantified by semi-quantitative RT-PCR. The expression levels NtCESA1 and NtCESA2 in transgenic plants were higher than that in control plants. The transgenic lines also showed a significant increase in cellulose content, suggesting that PeMYB-like may enhance cellulose biosynthesis through the upregulation of genes encoding enzymes involved in cellulose biosynthesis in plant stems. Our study indicated that there may be a close link between PeMYB-like gene expression and cellulose biosynthesis; moreover, BR signaling pathway might be involved in cellulose biosynthesis processes through the regulation of the MYB gene transcription.

Keywords

MYB Phyllostachys edulis Cellulose Brassinosteroids 

Notes

Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (No. 2015ZCQ-CL-03).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Lignocellulosic Chemistry, College of Materials Science and TechnologyBeijing Forestry UniversityBeijingPeople’s Republic of China

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