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Isolation and characterization of the rice NPR1 promoter

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Abstract

NPR1 is a positive regulator of systemic acquired resistance in Arabidopsis and rice. Expression of the rice gene OsNPR1 is induced by salicylic acid (SA). To identify the region of the OsNPR1 promoter involved in response to SA, we carried out deletion mutagenesis of the region 1005 bp upstream of the OsNPR1 start codon. Cis-element analysis revealed that the OsNPR1 promoter contains W-boxes and ASF1 motifs, both of which are known to be functional cis-elements of the WRKY and bZIP proteins, respectively. The deletion constructs 1005:LUC and 752:LUC, were induced by up to 4.3- and 3.8-fold, respectively, following SA treatment, suggesting that W-boxes and ASF1 motifs may play an important role in the strong induction of these constructs by SA. Using mutation analysis, we also showed that both the W-box and ASF1 motif are necessary for SA-induced expression of OsNPR1.

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Abbreviations

JA:

Jasmonic acid

SA:

Salicylic acid

SAR:

Systemic acquired resistance

Xoo :

Xanthomonas oryzae pv. oryzae

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Acknowledgments

This work was supported in part by a grant CG3134-1 from the Twenty-first Century Frontier Crop Functional Genomics and two grants from the National Academy of Agricultural Sciences (NAAS), Rural Development Administration (RDA) to Duk-Ju Hwang.

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  1. National Academy of Agricultural Sciences, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Seon-Hee Hwang & Duk-Ju Hwang

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  1. Seon-Hee Hwang
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  2. Duk-Ju Hwang
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Correspondence to Duk-Ju Hwang.

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Hwang, SH., Hwang, DJ. Isolation and characterization of the rice NPR1 promoter. Plant Biotechnol Rep 4, 29–35 (2010). https://doi.org/10.1007/s11816-009-0116-5

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