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His-tagged protein immobilization on cationic ferrite magnetic nanoparticles

  • Biotechnology
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Abstract

Magnetic nanoparticles have been applied in various fields because of their interesting magnetic properties. Immobilization on magnetic nanoparticles is a very important step in functionalizing them. We examined protein immobilization efficiency using interactions between his-tagged enhanced green fluorescence protein and affordable cationic ferrite magnetic nanoparticles for the first time. Four types of ferrite magnetic nanoparticles were verified: cobalt iron oxide, copper iron oxide, nickel iron oxide, and iron (III) oxide as negative controls. Among the four ferrite magnetic nanoparticles, copper ferrite magnetic nanoparticle was confirmed to have the highest immobilization efficiency at 3.0 mg proteins per gram ferrite magnetic nanoparticle and 78% of total enhanced green fluorescence protein. In addition, the maximum binding efficiency was determined for copper ferrite magnetic nanoparticle. Consequently, this newly verified his-tag-immobilizing capacity of copper ferrite magnetic nanoparticle could provide a facile, capable, and promising strategy for immobilizing his-tagged proteins or peptides with high purity for biosensors, magnetic separation, or diagnostics.

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References

  1. X. M. Li, J.R. Wei, K. E. Aifantis, Y. B. Fan, Q. L. Feng, F. Z. Cui and F. Watari, J. Biomed. Mater. Res. A, 104, 1285 (2016).

    Article  CAS  PubMed  Google Scholar 

  2. I. S. Lee, N. Lee, J. Park, B. H. Kim, Y. W. Yi, T. Kim, T. K. Kim, I. H. Lee, S. R. Paik and T. Hyeon, J. Am. Chem. Soc., 128, 10658 (2006).

    Article  CAS  PubMed  Google Scholar 

  3. J. L. Gu, H. F. Tong and L. Y. Sun, Biotechnol. Bioproc. E., 22, 76 (2017).

    Article  CAS  Google Scholar 

  4. I. M. El-Sherbiny, N. M. Elbaz, M. Sedki, A. Elgammal and M. H. Yacoub, Nanomedicine, 12, 387 (2017).

    Article  CAS  PubMed  Google Scholar 

  5. C. Sun, J. S. Lee and M. Zhang, Adv. Drug. Deliv. Rev., 60, 1252 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. J. P. Fortin, C. Wilhelm, J. Servais, C. Menager, J. C. Bacri and F. Gazeau, J. Am. Chem. Soc., 129, 2628 (2007).

    Article  CAS  PubMed  Google Scholar 

  7. A. H. Lu, E. L. Salabas and F. Schuth, Angew. Chem. Int. Ed., 46, 1222 (2007).

    Article  CAS  Google Scholar 

  8. J. Xu, J. Sun, Y. Wang, J. Sheng, F. Wang and M. Sun, Molecules, 19, 11465 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. C. Xu, K. Xu, H. Gu, R. Zheng, H. Liu, X. Zhang, Z. Guo and B. Xu, J. Am. Chem. Soc., 126, 9938 (2004).

    Article  CAS  PubMed  Google Scholar 

  10. H.Y. Park, M. J. Schadt, L. Wang, I. S. Lim, P. N. Njoki, S. H. Kim, M.Y. Jang, J. Luo and C. J. Zhong, Langmuir, 23, 9050 (2007).

    Article  CAS  PubMed  Google Scholar 

  11. W. Wang, Y. Xu, D. I. Wang and Z. Li, J. Am. Chem. Soc., 131, 12892 (2009).

    Article  CAS  PubMed  Google Scholar 

  12. C. Xu, K. Xu, H. Gu, X. Zhong, Z. Guo, R. Zheng, X. Zhang and B. Xu, J. Am. Chem. Soc., 126, 3392 (2004).

    Article  CAS  PubMed  Google Scholar 

  13. J. B. Yang, K. F. Ni, D. Z. Wei and Y.H. Ren, Biotechnol. Bioproc. E., 20, 901 (2015).

    Article  CAS  Google Scholar 

  14. Z. Rashid, H. Naeimi, A. H. Zarnani, F. Mohammadi and R. Ghahremanzadeh, Mat. Sci. Eng. C-Mater., 80, 670 (2017).

    Article  CAS  Google Scholar 

  15. Y. Zhou, S.F. Yuan, Q. Liu, D.D. Yan, Y. Wang, L. Gao, J. Han and H. F. Shi, Sci. Rep.-Uk, 7, 41741 (2017).

    Article  CAS  Google Scholar 

  16. J. Lee and J. H. Chang, Nanoscale Res. Lett., 9, 647 (2014).

    Article  CAS  Google Scholar 

  17. T. Kobayashi, N. Morone, T. Kashiyama, H. Oyamada, N. Kurebayashi and T. Murayama, PLoS One, 3, e3822 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. M.M. Bradford, Anal. Biochem., 72, 248 (1976).

    Article  CAS  PubMed  Google Scholar 

  19. E. E. Balint, J. Petres, M. Szabo, C. K. Orban, L. Szilagyi and B. Abraham, J. Fluoresc., 23, 273 (2013).

    Article  CAS  PubMed  Google Scholar 

  20. V. Gaberc-Porekar and V. Menart, J. Biochem. Biophys. Methods, 49, 335 (2001).

    Article  CAS  PubMed  Google Scholar 

  21. E.K. Ueda, P.W. Gout and L. Morganti, J. Chromatogr. A, 988, 1 (2003).

    Article  CAS  PubMed  Google Scholar 

  22. J.A. Bornhorst and J. J. Falke, Methods Enzymol., 326, 245 (2000).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. J. Arnau, C. Lauritzen, G.E. Petersen and J. Pedersen, Protein Expres. Purif., 48, 1 (2006).

    Article  CAS  Google Scholar 

  24. G. Nabiyouni, M. J. Fesharaki, M. Mozafari and J. Amighian, Chin. Phys. Lett., 27, 126401 (2010).

    Article  CAS  Google Scholar 

  25. K. Venkatesan, D. Rajan Babu, M.P. Kavya Bai, R. Supriya, R. Vidya, S. Madeswaran, P. Anandan, M. Arivanandhan and Y. Hayakawa, Int. J. Nanomedicine, 10 Suppl 1, 189 (2015).

    CAS  PubMed  PubMed Central  Google Scholar 

  26. B. L. Liu, Y. P. Fu and M. L. Wang, J. Nanosci. Nanotechnol., 9, 1491 (2009).

    Article  CAS  PubMed  Google Scholar 

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  1. Equal contribution.

Authors and Affiliations

  1. Division of Bioengineering, Incheon National University, Incheon, 22012, Korea

    Sung Jin Park, SeungYeon Kim, Seung Hoon Kim, Kyung Min Park & Byeong Hee Hwang

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  1. Sung Jin Park
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  2. SeungYeon Kim
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  3. Seung Hoon Kim
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  4. Kyung Min Park
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  5. Byeong Hee Hwang
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Correspondence to Kyung Min Park or Byeong Hee Hwang.

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Park, S.J., Kim, S., Kim, S.H. et al. His-tagged protein immobilization on cationic ferrite magnetic nanoparticles. Korean J. Chem. Eng. 35, 1297–1302 (2018). https://doi.org/10.1007/s11814-018-0043-7

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  • DOI: https://doi.org/10.1007/s11814-018-0043-7

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