Skip to main content
SpringerLink
Log in

Purification and characterization of angiotensin I converting enzyme inhibition peptides from sandworm Sipunculus nudus

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

Three angiotensin I converting enzyme (ACE) inhibition peptides were isolated from sandworm Sipunculus nudus protein hydrolysate prepared using protamex. Consecutive purification methods, including size exclusion chromatography and reverse-phase high performance liquid chromatography (RP-HPLC), were used to isolate the ACE inhibition peptides. The amino acid sequences of the peptides were identified as Ile-Asn-Asp, Val-Glu-Pro-Gly and Leu-Ala-Asp-Glu-Phe. The IC50 values of the purified peptides for ACE inhibition activity were 34.72 μmol L−1, 20.55 μmol L−1 and 22.77 μmol L−1, respectively. These results suggested that S. nudus proteins contain specific peptides that can be released by enzymatic hydrolysis. This study may provide an experimental basis for further systematic research, rational development and clinical utilization of sandworm resources.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bougatef, A., Nedjar-Arroume, N., Ravallec-Plé, R., Leroy, Y., Guillochon, D., Barkia, A., and Nasri, M., 2008. Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. Food Chemistry, 111: 350–356.

    Article  Google Scholar 

  • Byun, H. G., and Kim, S. K., 2001. Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin. Process Biochemistry, 36: 1155–1162.

    Article  Google Scholar 

  • Chen, J., Okada, T., Muramoto, K., Suetsuna, K., and Yang, S., 2003. Identification of angiotensin I-converting enzyme inhibitory peptides derived from the peptic digest of soybean protein. Journal of Food Biochemistry, 26: 543–554.

    Article  Google Scholar 

  • Clemente, A., 2000. Enzymatic protein hydrolysates in human nutrition. Trends in Food Science & Technology, 11: 254–262.

    Article  Google Scholar 

  • Hartmann, R., and Meisel, H., 2007. Food-derived peptides with biological activity: From research to food applications. Current Opinion in Biotechnology, 18: 163–169.

    Article  Google Scholar 

  • He, H. L., Chen, X. L., Sun, C. Y., Zhang, Y. Z., and Zhou, B. C., 2006. Analysis of novel angiotensin I-converting enzyme inhibitory peptides from protease-hydrolyzed marine shrimp Acetes chinensis. Journal of Peptide Science, 12: 726–733.

    Article  Google Scholar 

  • Hernández-Álvarez, A. J., Carrasco-Castilla, J., Dávila-Ortiz, G., Alaiz, M., Girón-Calle, J., Vioque-Peña, J., Jacinto-Hernández, C., and Jiménez-Martínez, C., 2013. Angiotensin-converting enzyme-inhibitory activity in protein hydrolysates from normal and anthracnose disease-damaged Phaseolus vulgaris seeds. Journal of the Science of Food and Agriculture, 93: 961–966.

    Article  Google Scholar 

  • Li, G. H., Le, G. W., Shi, Y. H., and Shrestha, S., 2004. Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiology and pharmacological effects. Nutrition Research, 24: 469–486.

    Article  Google Scholar 

  • Liu, T., Wu, H., Zhao, C. Y., Xie, J., and Lin, Y., 2012. Analyse of Sipunculus nudus’s composition and study of its craft of enzymolysis. Food Industry, 33: 71–74.

    Google Scholar 

  • Liu, X., Zhang, M., Jia, A., Zhang, Y., Zhu, H., Zhang, C., Sun, Z., and Liu, C., 2013. Purification and characterization of angiotensin I converting enzyme inhibitory peptides from jellyfish Rhopilema esculentum. Food Research International 50: 339–343.

    Article  Google Scholar 

  • Liu, X., Zhang, M., Shi, Y., Qiao, R., Tang, W., and Sun, Z., 2016. Produciton of the angiotensin I converting enzyme inhibitory peptides and isolation of four novel peptides from jellyfish (Rhopilema esculentum) protein hydrolysate. Journal of the Science of Food and Agirculture, 96: 3240–3248.

    Article  Google Scholar 

  • Matsui, T., Li, C. H., and Osajima, Y., 1999. Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ. Journal of Peptides Sciences, 5: 289–297.

    Google Scholar 

  • Ondetti, M. A., Rubin, B., and Cushman, D. W., 1977. Design of specific inhibitor of angitotensin-converting enzyme–New class of orally active antihypertensive agents. Science, 196: 441–444.

    Article  Google Scholar 

  • Su, J., Jiang, L., Wu, J., Liu, Z., and Wu, Y., 2016. Anti-tumor and anti-virus activity of polysaccharides extracted from Sipunculus nudus (SNP) on Hepg2.2.15. International Journal of Biological Macromolecules, 87: 597–602.

    Article  Google Scholar 

  • Sun, X., Yang, J., Xu, Y., Liu, H., and Ren, Y., 2015. Study on hydrolysis conditions of Sipunculus nudus by response methodology. Food & Machinery, 31: 238–242.

    Google Scholar 

  • Wijesekara, I., and Kim, S. K., 2010. Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: Prospects in the pharmaceutical industry. Marine Drugs, 8: 1080–1093.

    Article  Google Scholar 

  • Wu, H., He, H. L., Chen, X. L., Sun, C. Y., Zhang, Y. Z., and Zhou, B. C., 2008. Purification and identification of novel angiotensin-I-converting enzyme inhibitory peptides from shark meat hydrolysate. Process Biochemistry, 43: 457–461.

    Article  Google Scholar 

  • Zhang, C. X., and Dai, Z. R., 2011. Anti-hypoxia activity of a polysaccharide extracted from the Sipunculus nudus L. International Journal of Biological Macromolecules, 49: 523–526.

    Article  Google Scholar 

  • Zhang, C. X., Dai, Z. R., and Cai, Q. X., 2011. Anti-inflammatory and anti-nociceptive activities of Sipunculus nudus L. extract. Journal of Ethnopharmacology, 137: 1177–1182.

    Article  Google Scholar 

  • Zhao, Y., Li, B., Liu, Z., Dong, S., Zhao, X., and Zeng, M., 2007. Antihypertensive effect and purification of an ACE inhibition peptide from sea cucumber gelatin hydrolysate. Process Biochemistry, 42: 1586–1591.

    Article  Google Scholar 

Download references

Acknowledgements

This work is supported by research grant of Guangxi Key Laboratory Traditional Chinese Medicine Quality Standards (No. GXGZZK201501) and the Open Research Fund Program of Guangxi Key Laboratory of Marine Biotechnology (No. GLMBT-201407), and partly supported by Shanghai Fengxian District Science and Technology Project (Nos. 20141001 and 20151205), Shanghai No. 6 People’s Medical Group Project and research project of Shanghai municipal health and Family Planning Commission (No. 201540027).

Author information

Authors and Affiliations

  1. Guangxi Institute of Traditional Medical and Pharmaceutical Science & Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning, 530022, P. R. China

    Xueping Sun & Buming Liu

  2. Fengxian Hospital Affiliated to Southern Medical University, Shanghai, 201499, P. R. China

    Man Wang & Zhenliang Sun

  3. Guangxi Institute of Oceanology, Guangxi Key Laboratory of Marine Biotechnology, Beihai, 536000, P. R. China

    Zhenliang Sun

Authors
  1. Xueping Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Man Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Buming Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhenliang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenliang Sun.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, X., Wang, M., Liu, B. et al. Purification and characterization of angiotensin I converting enzyme inhibition peptides from sandworm Sipunculus nudus . J. Ocean Univ. China 16, 911–915 (2017). https://doi.org/10.1007/s11802-017-3293-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-017-3293-9

Key words

Search

Navigation