A tissue culture system for callus formation and plant regeneration using tuber discs of Dioscorea tokoro Makino, a pharmaceutical yam species

Abstract

Yam (Dioscorea sp.) is a recalcitrant plant species with respect to plant regeneration in tissue culture. We report here on callus formation and plantlet regeneration in the pharmaceutical yam species D. tokoro Makino. Explants of leaves, nodes, internodes, roots, and tuber discs of three lines of D. tokoro were cultured on media containing the synthetic auxins 2,4-dichlorophenoxyacetic acid or picloram at 10 µM. Explants of nodes, roots, and tuber discs formed calluses at high frequency in all lines we examined, regardless of type of auxin added to the media. However, explants of leaves and internodes rarely formed calluses. Some calluses derived from tuber discs differentiated shoots through the formation of bud-like structures by culture on media with or without 10 µM 6-benzyladenine. The T-1110 line showed the highest shoot formation efficiency. The regenerants from the calluses derived from tuber discs successfully acclimatized to a greenhouse, grew normally and ultimately formed tubers in soil.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzyladenine

MS:

Murashige and Skoog

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Acknowledgements

This work was conducted as part of the international collaborative research project, “Use of genomic information and molecular tools for yam germplasm utilization and improvement for West Africa (EDITS-Yam)” funded by the Japan International Research Center for Agricultural Sciences (JIRCAS). The authors thank Dr. Natsume (Iwate Biotechnology Research Center) for kindly providing the yam materials, and are grateful to Drs. Hiroko Takagi and Satoru Muranaka (JIRCAS) for their valuable suggestions and continuous encouragements. We appreciate the excellent technical support provided by Suwako Yajima, Mariko Tamanaha, Saori Hoshi, Yoko Komuro, Akiko Nemoto and Ayumi Nakamine (JIRCAS).

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Correspondence to Takuma Ishizaki.

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This work was conducted as part of the international collaborative research project, “Use of genomic information and molecular tools for yam germplasm utilization and improvement for West Africa (EDITS-Yam)” funded by the Japan International Research Center for Agricultural Sciences (JIRCAS).

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The author declares no conflict of interest.

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Ishizaki, T. A tissue culture system for callus formation and plant regeneration using tuber discs of Dioscorea tokoro Makino, a pharmaceutical yam species. Biologia (2021). https://doi.org/10.1007/s11756-021-00701-4

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Keywords

  • Dioscorea tokoro Makino
  • In vitro plantlet regeneration
  • Tuber disc
  • Yam