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Lipids

, Volume 47, Issue 1, pp 47–58 | Cite as

Dietary CLA Combined with Palm Oil or Ovine Fat Differentially Influences Fatty Acid Deposition in Tissues of Obese Zucker Rats

  • Susana V. Martins
  • Paula A. Lopes
  • Susana P. Alves
  • Cristina M. Alfaia
  • Matilde F. Castro
  • Rui J. B. Bessa
  • José A. M. PratesEmail author
Original Article
First Online:

Abstract

The effect of dietary conjugated linoleic acid (CLA) supplementation in combination with fat from vegetable versus animal origin on the fatty acid deposition, including that of individual 18:1 and 18:2 (conjugated and non-conjugated) isomers, in the liver and muscle of obese rats was investigated. For this purpose, 32 male Zucker rats were randomly assigned to one of four diets containing palm oil or ovine fat, supplemented or not with 1% of 1:1 cis(c)9,trans(t)11 and t10,c12 CLA isomers mixture. Total fatty acid content decreased in the liver and muscle of CLA-fed rats. In the liver, CLA increased saturated fatty acids (SFA) in 11.9% and decreased monounsaturated fatty acids (MUFA) in 6.5%. n-3 Polyunsaturated fatty acids (PUFA) relative proportions were increased in 30.6% by CLA when supplemented to the ovine fat diet. In the muscle, CLA did not affect SFA but decreased MUFA and PUFA percentages. The estimation of Δ9-indices 16 and 18 suggested that CLA inhibited the stearoyl-CoA desaturase activity in the liver (a decrease of 13–38%), in particular when supplemented to the ovine fat diet. Concerning CLA supplementation, the t10,c12 isomer percentage was 60–80% higher in the muscle than in the liver. It is of relevance that rats fed ovine fat, containing bio-formed CLA, had more c9,t11 CLA isomer deposited in both tissues than rats fed palm oil plus synthetic CLA. These results highlight the importance to further clarify the biological effects of consuming foods naturally enriched in CLA, alternatively to CLA dietary supplementation.

Keywords

Conjugated linoleic acid Palm oil Ovine fat Fatty acid composition Obese Zucker rat Liver Muscle 

Abbreviations

Ag+-HPLC

Silver ion-high pressure liquid chromatography

c9,t11

cis9,trans11 CLA isomer

CLA

Conjugated linoleic acid

FAME

Fatty acid methyl esters

GC

Gas chromatography

MUFA

Monounsaturated fatty acids

O

Ovine fat group

OCLA

Ovine fat plus 1% CLA group

P

Palm oil group

PCLA

Palm oil plus 1% CLA group

PUFA

Polyunsaturated fatty acids

SCD

Stearoyl-CoA desaturase

SFA

Saturated fatty acids

t10,c12

trans10,cis12 CLA isomer

TFA

trans fatty acids

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Notes

Acknowledgments

The CLA oil was a generous gift from PharmaNutrients Inc. (Gurnee, IL, USA). This study was supported by the Fundação para a Ciência e a Tecnologia (FCT, Lisbon, Portugal) Grant POCTI/CVT/2002/44750 and individual fellowships to Susana Martins and Susana Alves, SFRH/BD/2005/22566 and SFRH/BD/2007/37793, respectively. Paula A. Lopes is a researcher from the program “Ciência 2008” from FCT.

Conflict of interest

None.

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Copyright information

© AOCS 2011

Authors and Affiliations

  • Susana V. Martins
    • 1
  • Paula A. Lopes
    • 1
  • Susana P. Alves
    • 2
    • 3
  • Cristina M. Alfaia
    • 1
  • Matilde F. Castro
    • 4
  • Rui J. B. Bessa
    • 1
    • 2
  • José A. M. Prates
    • 1
    Email author
  1. 1.Secção de Bioquímica, CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade TécnicaPólo Universitário do Alto da AjudaLisbonPortugal
  2. 2.Unidade de Produção Animal, L-INIAINRB I.P.Vale de SantarémPortugal
  3. 3.REQUIMTE, Instituto de Ciências Biomédicas Abel SalazarUniversidade do PortoVairão, VCPortugal
  4. 4.Faculdade de FarmáciaiMed.ULLisbonPortugal

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