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Dynamics of circulating microparticles in obesity after weight loss

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Abstract

A definitive relationship between adiposity and MP production is yet to be demonstrated. The aim of our study was to prospectively evaluate the levels of microparticles (MP) in a group of 20 III degree obese patients before and after weight loss. Plasma levels of annexin V-MP, endothelial-derived MP, platelet-derived MP (CD61+ and P-Selectin+), leukocyte-derived MP, tissue factor-bearing (TF+) and CD36+MP were prospectively measured in 20 patients with III degree obesity (BMI ≥ 40 kg/m2) before (T0) and 3 (T3) and 12 (T12) months after sleeve gastrectomy (SLG). Obese patients had lost 18 % of their body weight at T3 and 41 % at T12. We find that considering all MP, except for endothelial-derived MP, which had significantly decreased at T3, all MP subtypes had significantly decreased at T12. At T12, subjects showed a higher median level of all types of MP, except endothelial-derived MP, compared to T3, but without a statistically significant difference. The percentages of reduction of all the MP were significantly correlated with the percentage of reduction of BMI. The reductions of leukocyte-derived, TF+ and CD36+MP were significantly correlated with the reduction of leptin. Moreover, the reductions of leukocyte-derived and CD36+MP were significantly correlated with hs-CRP decrease. The decrease of BMI post-SLG in morbid obesity was matched with a decrease of circulating MP of endothelial, platelet, leukocyte origin, TF+ and CD36+. A trend of slight increase in all MP subtypes, except endothelial-derived, was detected 12 months after gastrectomy, indicating a possible underlying slow low-grade inflammatory/hypercoagulability state from adipose tissue before the potential overt weight gain.

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    Authors and Affiliations

    1. Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine (DIMED), University of Padua, Via Ospedale Civile 105, 35100, Padua, Italy

      Elena Campello, Eva Zabeo, Claudia M. Radu, Luca Spiezia, Sabrina Gavasso, Cristiana Bulato, Roberto Vettor & Paolo Simioni

    2. Week Surgery, Bariatric Unit, University of Padua, Padua, Italy

      Mirto Foletto & Luca Prevedello

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    1. Elena Campello
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    2. Eva Zabeo
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    3. Claudia M. Radu
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    Correspondence to Elena Campello.

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    Statement of human and animal rights

    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the author.

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    Each patient considered for the study gave his/her personal informed consent at the time of enrollment.

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    E. Campello and E. Zabeo have contributed to this work.

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    Campello, E., Zabeo, E., Radu, C.M. et al. Dynamics of circulating microparticles in obesity after weight loss. Intern Emerg Med 11, 695–702 (2016). https://doi.org/10.1007/s11739-016-1397-7

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