Advertisement

Internal and Emergency Medicine

, Volume 8, Issue 6, pp 497–501 | Cite as

High correlation of babA 2 -positive strains of Helicobacter pylori with the presence of gastric cancer

  • Amin Talebi Bezmin Abadi
  • Tarang TaghvaeiEmail author
  • Ashraf Mohabbati Mobarez
  • Giuseppina Vaira
  • Dino Vaira
IM - ORIGINAL

Abstract

Helicobacter pylori (H. pylori) is a Gram-negative bacterium that is well known in the involvement of chronic inflammation in the gastric mucosa of the human stomach. Several studies have investigated the possible role of H. pylori presence in different gastroduodenal disorders with conflicting results. This study aimed to further investigate such a field. Helicobacter pylori strains were cultured from 160 patients (mean age of 42 years; range 15–75; 90 were male, and 70 were female) [40 gastric cancer (GC), 55 duodenal ulcer (DU) and 65 non-ulcer dyspepsia (NUD)]. In this study, allelic variants of iceA 1 , iceA 2 and babA 2 were identified by polymerase chain reaction. The overall prevalence of babA 2 gene was 40.6% (65/160). The prevalence of babA 2 gene was 95% with gastric cancer, 18.1% with duodenal ulcer and 26.1% with non ulcer dyspepsia, respectively. The prevalence of babA 2 in GC patients was significantly higher as compared to either NUD or UD patients (P = 0.0004), while no statistical significance was found between the latter two patient groups. Our study finds that babA 2 and iceA 1 genes are more prevalent in GC compared to either NUD or DU patients in Iran.

Keywords

Helicobacter pylori babA2 iceA1 iceA2 Iran 

Notes

Acknowledgments

We would like to acknowledge Ali Ghasemzadeh from Marquette University, Milwaukee, USA for his genuine and helpful contributions in our project. This project was supported by Talent Student grant which gifted to Amin talebi at 2007 by Research, Technology and Sciences Ministry of Islamic Republic of Iran.

Conflict of interest

None.

References

  1. 1.
    Blaser M, Atherton JC (2004) Helicobacter pylori persistence: biology and disease. J Clin Invest 113:321–333PubMedGoogle Scholar
  2. 2.
    Goodman KJ, Correa P (2000) Transmission of Helicobacter pylori among siblings. Lancet 355:358–362PubMedCrossRefGoogle Scholar
  3. 3.
    Kusters JG, Arnoud Vliet HM, Kuipers EJ (2006) Pathogenesis of Helicobacter pylori Infection. Clin Microbiol Rev 19:449–490PubMedCrossRefGoogle Scholar
  4. 4.
    Costa C, Figueiredo C, Touati E (2009) Pathogenesis of Helicobacter pylori Infection. Helicobacter 14(Suppl. 1):15–20PubMedCrossRefGoogle Scholar
  5. 5.
    Wang G, Maier RJ (2009) A RecB-like helicase in Helicobacter pylori is important for DNA repair and host colonization. Infect Immun 77:286–291PubMedCrossRefGoogle Scholar
  6. 6.
    Amundsen SK, Fero J, Hansen LM et al (2008) Helicobacter pyloriAddAB helicase-nuclease and RecA promote recombination related DNA repair and survival during stomach colonization. Mol Microbiol 69:994–1007PubMedCrossRefGoogle Scholar
  7. 7.
    Lamarque D, Peek RM (2003) Pathogenesis of Helicobacter pylori infection. Helicobacter 8(suppl):21–30PubMedCrossRefGoogle Scholar
  8. 8.
    Ilver D, Arnqvist A, Ogren J, Frick IM, Kersulyte D, Incecik ET et al (1998) Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science 279:373–377PubMedCrossRefGoogle Scholar
  9. 9.
    Gerhard M, Lehn N, Neumayer N, Boren T, Rad R, Schepp W et al (1999) Clinical relevance of the Helicobacter pylori gene for blood group antigen-binding adhesin. Proc Natl Acad Sci USA 96:12778–12783PubMedCrossRefGoogle Scholar
  10. 10.
    Peek RM, Thompson SA, Donahue JP, Tham KT, Atherton JC, Blaser MJ, Miller GG (1998) Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA that is associated with clinical outcome. Proc Assoc Am Physicians 110:531–544PubMedGoogle Scholar
  11. 11.
    Van Doorn LJ, Figueiredo C, Sanna R, Plaisier A, Schneeberger P, De Boer W, Quint W (1998) Clinical relevance of cagA, vacA and iceA status of Helicobacter pylori. Gastroenterology 115:58–66PubMedCrossRefGoogle Scholar
  12. 12.
    Ito Y, Azuma T, Ito S (2000) Sequence analysis and clinical significance of the iceA gene from Helicobacter pylori strains in Japan. J Clin Microbiol 38:483–488PubMedGoogle Scholar
  13. 13.
    Mukhopadhyay AK, Kersulyte D, Jeong JN et al (2000) Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India. J Bacteriol 182:3219–3227PubMedCrossRefGoogle Scholar
  14. 14.
    Yamaoka Y, Kodama T, Gutierrez O, Kim JG, Kashima K, Graham DY (1999) Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol 37:2274–2279PubMedGoogle Scholar
  15. 15.
    Malekzadeh R, Derakhshan MH, Malekzadeh Z (2009) Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med 12:576–583PubMedGoogle Scholar
  16. 16.
    Dixon MF, Genta RM, Yardley JH, Correa P (1996) Classification and grading of gastritis. The updated Sydney system. International workshop on the histopathology of gastritis, Houston 1994. Am J Surg Pathol 20:1161–1181PubMedCrossRefGoogle Scholar
  17. 17.
    Yamazaki S, Yamakawa A, Okuda T, Ohtani M et al (2005) Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. J Clin Microbiol 43:3906–3916PubMedCrossRefGoogle Scholar
  18. 18.
    Podzorski RP, Podzorski DS, Wuerth A, Tolia V (2003) Analysis of the vacA, cagA, cagE, iceA, and babA2 genes in Helicobacter pylori from sixty-one pediatric patients from the Midwestern United States. Diagn Microbiol Infect Dis 46:83–88PubMedCrossRefGoogle Scholar
  19. 19.
    Xu Q, Blaser MJ (2001) Promoters of the CATG-specific methyltransferase gene hpyIM differ between iceA 1 and iceA 2 Helicobacter pylori strains. J Bacteriol 183:3875–3884PubMedCrossRefGoogle Scholar
  20. 20.
    Chomvarin C, Namwat W, Chaicumpar K, Mairiang P et al (2008) Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis 12:30–36PubMedCrossRefGoogle Scholar
  21. 21.
    Miehlike S, Schuppler M, Frings C et al (2001) Helicobacter pylori vacA, iceA and cagA status and pattern of gastritis in patients with malignant and benign gastroduodenal disease. Am J Gastroenterol 4:1008–1030CrossRefGoogle Scholar
  22. 22.
    Nishiya D, Shimoyama T, Fukuda S et al (2000) Evaluation of the clinical relevance of the iceA 1 gene in patients with H. pylori infection in Japan. Scand J Gastroenterol 35:36–39PubMedCrossRefGoogle Scholar
  23. 23.
    Han YH, Liu WZ, Zhu HY, Xiao SD (2004) Clinical relevance of iceA and babA 2 genotypes of Helicobacter pylori in a Shanghai population. Chin J Dig Dis 5:181–185PubMedCrossRefGoogle Scholar
  24. 24.
    Kim SY, Woo CW, Lee YM, Son BR, Kim JW, Chae HB et al (2001) Genotyping cagA, vacA subtype, iceA 1, and babA of Helicobacter pylori isolates from Korean patients, and their association with gastroduodenal diseases. J Korean Med Sci 16:579–584PubMedGoogle Scholar
  25. 25.
    Ashour AA, Collares GB, Mendes EN, De Gusmao VR, Queiroz DM, Magalhaes PP et al (2001) iceA genotypes of Helicobacter pylori strains isolated from Brazilian children and adults. J Clin Microbiol 39:1746–1750PubMedCrossRefGoogle Scholar
  26. 26.
    Lai CH, Kuom CH, Chen YC, Chao FY, Poon SK, Chang CS, Wang WC (2002) High prevalence of cagA- and babA2-positive Helicobacter pylori clinical isolates in Taiwan. J Clin Microbiol 40:3860–3862PubMedCrossRefGoogle Scholar
  27. 27.
    Mizushima T, Toshiro S, Komatsu Y, Ishizuka J, Mototsgu K, Asaka M (2001) Clinical relevance of the babA 2 genotype of Helicobacter pylori in Japanese clinical isolates. J Clin Microbiol 39:2463–2465PubMedCrossRefGoogle Scholar
  28. 28.
    Oleastro M, Gerhard M, Lopes AI, Ramalho P, Cabral J, Sousa Guerreiro A, Monteiro L (2003) Helicobacter pylori virulence genotypes in Portuguese children and adults with gastroduodenal pathology. Eur J Clin Microbiol Infect Dis 22:85–91PubMedGoogle Scholar
  29. 29.
    Oliveira AG, Santos A, Guerra JB et al (2003) babA2- and cagA-positive Helicobacter pylori strains are associated with duodenal ulcer and gastric carcinoma in Brazil. J Clin Microbiol 41:3964–3966PubMedCrossRefGoogle Scholar
  30. 30.
    Gatti LL, Proenc JL, Marques Payao SL et al (2006) Prevalence of Helicobacter pylori cagA, iceA and babA 2 alleles in Brazilian patients with upper gastrointestinal diseases. Acta Trop 100:232–240PubMedCrossRefGoogle Scholar
  31. 31.
    Torres LE, Melian K, Moreno A et al (2009) Prevalence of vacA, cagA and babA 2 genes in Cuban Helicobacter pylori isolates. World J Gastroenterol 14:204–210CrossRefGoogle Scholar
  32. 32.
    Eshaghi M, GhasemianSafaei H, Havaei A, Navabakbar A et al (2008) Assessment of babA 2 genotype frequency in H. Pylori and its relationship with digestive tract diseases in patients in Isfahan’s Alzahra Hospital. Sci J Kurdistan Univ Med Sci 14:21–27Google Scholar
  33. 33.
    Talebi Bezmin Abadi A, Taghvaei T, Vaira D (2010) Considerable use of furazolidone in Iran. Saudi J Gastroenterol 16:308–309CrossRefGoogle Scholar
  34. 34.
    Talebi Bezmin Abadi A, Mohabati Mobarez A, Taghvaei T, Wolfram L (2010) Antibiotic resistance of Helicobacter pylori in Mazandaran, North of Iran. Helicobacter 15:505–509PubMedCrossRefGoogle Scholar

Copyright information

© SIMI 2011

Authors and Affiliations

  • Amin Talebi Bezmin Abadi
    • 1
  • Tarang Taghvaei
    • 3
    Email author
  • Ashraf Mohabbati Mobarez
    • 1
  • Giuseppina Vaira
    • 2
  • Dino Vaira
    • 2
  1. 1.Department of Bacteriology, School of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Internal Medicine and GastroenterologyS. Orsola HospitalBolognaItaly
  3. 3.Endoscopic RoomTooba ClinicSariIran

Personalised recommendations