Identification, evolution and expression analyses of Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene family in wheat (Triticum aestivum L.)

  • Lingyue Qin
  • Yuanxia Xue
  • Ying Fei
  • Lingfeng Zeng
  • Shushen Yang
  • Xiping Deng
Original Article


Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) serves as a plentiful leaf protein which functions in both eukaryote and prokaryote photosynthesis. The small subunits of Rubisco (RBCS) exist as a multigene family which regulates the catalytic efficiency of holoenzyme. Here, 20 RBCS family genes were identified in Triticum aestivum genome, and were clustered into 4 clades according to phylogenetic analysis. On the basis of the identified 9 and 8 RBCSs in Triticum urartu and Aegilops tauschii, homology analysis revealed some TaRBCS genes were orthologous to TuRBCSs and AetRBCSs, and the number of in-paralog pairs between RBCSs in wheat were much more than that in T. urartu or A. tauschii. Gene structure, protein motif and cis-acting element analysis exhibited that TaRBCSs in each clade shared some identity. The in silico expression of RBCS genes showed that RBCSs mainly expressed in leaf, flower and caryopsis. Quantitative real-time PCR analysis showed that TaRBCSs were remarkably responsive to drought, salt, ABA and darkness stresses. The work comprehensively studies the RBCS family genes in wheat, and lays the foundation for subsequent functional research of TaRBCSs.


RBCS Wheat Phylogenetic analysis Gene expression Abiotic stress 



This work was supported by The National Nature Science Foundation of China (No. 31671609 and No. 51479189), The State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Institute of Soil and Water Conversation, Chinese Academy of Science (No. 10502), and The National Basic Research Program of China (No. 2015CB150402).

Supplementary material

11738_2018_2658_MOESM1_ESM.pdf (1.9 mb)
Fig. S1 Distribution of RBCSs throughout all clades. RBCSs in wheat, T. urartu, Ae. tauschii, B. distachyon, rice, poplar, and Arabidopsis were divided into 5 clades. Sum of RBCSs in particular genome was presented by number in the bracket (PDF 1989 kb)
11738_2018_2658_MOESM2_ESM.pdf (4.8 mb)
Fig. S2 The number of ortholog pairs between T. urartu or Ae. tauschii genome and wheat sub-genomes. Numbers besides straight line indicate the sum of ortholog pairs between particular genome and sub-genome (PDF 4882 kb)
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Fig. S3 Orthologs between RBCS genes in T. urartu and Ae. tauschi (PDF 2048 kb)
11738_2018_2658_MOESM4_ESM.pdf (1.1 mb)
Fig. S4 Multiple alignment of Rubisco_small domain sequences in moss, gymnosperm, monocot or dicot. Conserved residues across all sequences are displayed in blue. The protein secondary structure α-helix A, B, and β-strands A to D are colored in green and yellow. The accession numbers of these RBCSs are BAA83481.1, BAR94274.1, EFJ27478.1, EFJ07320.1, XP_012074043.1, ABK25403.1, XP_010450913.1, CDX74532.1, AIF75325.1, AED94314.1, BAB19812.1, EMS44969.1, EMT21848.1, AAF07946.1 (PDF 1092 kb)
11738_2018_2658_MOESM5_ESM.pdf (4.8 mb)
Fig. S5 Details (width and site) for conserved motifs among RBCS family members detected by MEME. The maximum motif was set as 50 (PDF 4928 kb)
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Supplementary material 6 (PDF 135 kb)
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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.College of Life ScienceNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Yangling Vocational and Technical CollegeYanglingPeople’s Republic of China
  3. 3.Institute of Soil and Water ConservationChinese Academy of SciencesYanglingPeople’s Republic of China

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