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Estimating the contribution of arbuscular mycorrhizal fungi to drought tolerance of potted olive trees (Olea europaea)

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Abstract

The aim of the present study is to investigate the contribution of mycorrhization to the resilience of olive trees to drought. One-year-old olive plants were inoculated (Myc+) or not (Myc−) with arbuscular mycorrhizal fungi (AMF), and subjected to a 40-day-drought period. At regular intervals of the watering-off period and after rehydration period, water relations and gas exchanges parameters were measured. Similarly, the total soluble sugars, proline, and mineral nutrients concentrations were determined. The results revealed that Myc+ plants were less affected by drought than Myc− plants proving the involvement of the AMF in the alleviation of drought impact on olive tree. In fact, the turgor potential (Ψp) in Myc+ plants exhibited positive values during the whole treatment period, while Ψp in Myc− plants was negative mainly under severe stress intensity. Moreover, the stomatal function of Myc+ plants was less affected by drought compared to Myc− plants. The maximum of mycorrhizas relative drought alleviation rate (RDAR) was estimated to be 40% for Ψpd and RWC, 36% for the osmotic potential (ΨS), 86% for Ψp, 16% for gs, and 27% for E. The osmotic adjustment by proline was earlier in Myc+ plants than in Myc− ones. The inoculation with AMF also improved mineral uptake (K, N, Zn, and Fe). After 40 days of drought, Myc+ plants survive but not Myc− ones. In addition, the restoration of the irrigation permitted the Myc+ plants to recuperate from severe drought stress. To sum up, inoculation of young olive trees with the AMF improved their resilience to drought.

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Abbreviations

RDAR:

Relative drought alleviation rate

\( {\text{DI}}_{{{\text{Myc}}^{ - } }} \) :

Drought impact in non-mycorrhizal plants

\( {\text{DI}}_{{{\text{Myc}}^{ + } }} \) :

Drought impact in mycorrhizal plants

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Authors and Affiliations

  1. Research Unit of Biodiversity and Valorization of Bioresources in Arid Zones (BVBZA), Faculty of Sciences of Gabes, University of Gabes, City Erriadh, Zrig, 6072, Gabes, Tunisia

    Sarra Ouledali, Mustapha Ennajeh, Ahlem Zrig & Habib Khemira

  2. INOCULUMplus Technopôle Agro-Environnement RD31, 21110, Bretenière, France

    Silvio Gianinazzi

  3. Center for Environmental Research and Studies (CERS), Jazan University, Jazan, 82817, Saudi Arabia

    Habib Khemira

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  1. Sarra Ouledali
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Correspondence to Mustapha Ennajeh.

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Communicated by M. H. Walter.

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Ouledali, S., Ennajeh, M., Zrig, A. et al. Estimating the contribution of arbuscular mycorrhizal fungi to drought tolerance of potted olive trees (Olea europaea). Acta Physiol Plant 40, 81 (2018). https://doi.org/10.1007/s11738-018-2656-1

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