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The effects of exogenous ABA applied to maize (Zea mays L.) roots on plant responses to chilling stress

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Abstract

This work aimed to discuss the effects of exogenous abscisic acid (ABA) on the root growth regulation of maize seedlings under chilling stress. The roots of the maize cultivar Zhengdan 958 were irrigated with ABA (10−7, 10−6, 10−5 and 10−4 M) at the third true leaf stage under chilling duration (0, 2, 4, 6, and 8 days). The biomass, the phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) enzyme activities, total phenolic and flavonoid contents, the ferric reducing ability of plasma (FRAP) antioxidant capacity, and 2,2-azinobis (3-ethlbenzothiazo-line-6-sulfonic acid) diammonium salt radical (ABTS·+) scavenging capacity of the roots of maize seedlings were measured after the treatment. The results showed that appropriate concentrations of exogenous ABA effectively enhanced root biomass, increased PAL and PPO enzyme activities, and significantly increased total phenolic contents and flavonoid contents. Moreover, the ABA markedly improved the FRAP antioxidant capacity and ABTS·+ scavenging capacity under low-temperature stress. These results indicate that ABA-treated maize seedlings are resistant to chilling stress and that the optimum concentration of ABA is 10−5 M. Exogenous applications of ABA have a concentration effect in alleviating chilling stress, in which low concentrations have a promoting effect and high concentrations have an inhibiting effect.

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Acknowledgements

This work was funded by the National Key Research and Development (R&D) Program of China (No. 2017YFD0300405).

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  1. College of Agronomy, Northeast Agricultural University, Harbin, 150030, China

    Li-xin Tian & Jing Li

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  1. Li-xin Tian
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Correspondence to Jing Li.

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Communicated by P. Wojtaszek.

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Tian, Lx., Li, J. The effects of exogenous ABA applied to maize (Zea mays L.) roots on plant responses to chilling stress. Acta Physiol Plant 40, 77 (2018). https://doi.org/10.1007/s11738-018-2655-2

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