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Aspirin priming circumvents the salinity-induced effects on wheat emergence and seedling growth by regulating starch metabolism and antioxidant enzyme activities

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Abstract

Though seed priming has been emerged as an effective and pragmatic approach, efforts are being made to discover and optimize the new priming agents which are cheaper and easily accessible to the farmers. Here, we established two independent experiments, to ascertain the role of aspirin priming in salinity tolerance of wheat. In the first experiment, various concentrations of aspirin (125, 250, 375, and 500 ppm) were examined for emergence and seedling growth of wheat. A non-primed control, hydropriming, and hydrogen peroxide priming treatments were also maintained for comparison. Among the different treatments, seeds primed with 125 and 250 ppm aspirin depicted better emergence, vigorous seedling growth, and higher starch metabolism. Therefore, these treatments were further used in the second experiment under salinity stress (10 dS m−1). Salinity stress caused delayed and erratic emergence hampered the shoot and root growth, chlorophyll contents, and enhanced the lipid peroxidation and phenolics content in wheat seedlings. However, wheat seed priming particularly with aspirin effectively alleviated the negative effects of salinity on most of the observed parameters. Aspirin priming also significantly enhanced the activities of antioxidant enzymes (catalase, peroxidase, and superoxide dismutase), and reduced oxidative stress in wheat seedlings. Vigorous growth and greater salinity tolerance of wheat seedlings derived from aspirin primed seeds were related with better starch metabolism, strong antioxidative defense system, and lower lipid peroxidation.

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References

  • Abdul-Baki BAA, Anderson JP (1973) Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci 13:227–234

    Article  CAS  Google Scholar 

  • Afzal I, Basra SMA, Farooq M, Nawaz A (2006) Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int J Agric Biol 8:23–28

    CAS  Google Scholar 

  • Ahanger MA, Tyagi SR, Wani MR, Ahmad P (2014) Drought tolerance: role of organic osmolytes, growth regulators, and mineral nutrients. Physiological mechanisms and adaptation strategies in plants under changing environment. Springer, New York, pp 25–55

    Chapter  Google Scholar 

  • Anjum SA, Tanveer M, Hussain S, Bao M, Wang L, Khan I, Ehsanullah Tung SA, Samad RA, Shahzad B (2015a) Cadmium toxicity in maize (Zea mays L.): consequences on antioxidative systems, reactive oxygen species and cadmium accumulation. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-015-4882-z

    Google Scholar 

  • Anjum SA, Tanveer M, Hussain S, Ullah E, Wang LC, Khan I, Samad RA, Tung SA, Anam M, Shahzad B (2015b) Morpho-physiological responses of two contrasting maize cultivars to cadmium toxicity. Clean Soil Air Water. https://doi.org/10.1002/clen.201400905

    Google Scholar 

  • Anjum SA, Ashraf U, Tanveer M, Khan I, Hussain S, Zohaib A, Abbas F, Saleem MF, Wang L (2017) Drought induced changes in growth, osmolyte accumulation and antioxidant metabolism of three maize hybrids. Front Plant Sci 8:69. https://doi.org/10.3389/fpls.2017.00069

    Article  PubMed  PubMed Central  Google Scholar 

  • AOSA (1983) Seed vigor hand testing book. Contribution No. 32 to the handbook on seed testing. Association of Official Seed Analysts (AOSA), Springfield

    Google Scholar 

  • AOSA (1990) Rules for testing seeds. Association of Official Seed Analysts (AOSA). J Seed Technol 12:1–112

    Google Scholar 

  • Bailly C, Benamar A, Corbineau F, Dome D (1996) Changes in malondialdehyde contents and in superoxide dismutase, catalase, glutathione reductase activities in sunflower seeds related to accelerated seed aging. Physiol Plant 97:104–110

    Article  CAS  Google Scholar 

  • Bradford KJ (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hortic Sci 21:1105–1112

    Google Scholar 

  • Chen W, Guo C, Hussain S, Zhu B, Deng F, Xue Y, Geng M, Wu L (2016) Role of xylo-oligosaccharides in protection against salinity-induced adversities in Chinese cabbage. Environ Sci Pollut Res 23:1254–1264

    Article  CAS  Google Scholar 

  • Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101

    Article  CAS  Google Scholar 

  • Dubios M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugar and related substances. Anal Chem 28:350–356

    Article  Google Scholar 

  • Egley GH, Paul RN, Vaughn KC, Duke SO (1983) Role of peroxidase in the development of water impermeable seed coats in Sida spinosa L. Planta 157:224–232

    Article  CAS  PubMed  Google Scholar 

  • Ellis RA, Roberts EH (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9:373–409

    Google Scholar 

  • Fahad S, Hussain S, Matloob A, Khan FA, Khaliq A, Saud S et al (2014) Phytohormones and plant responses to salinity stress: a review. Plant Growth Regul 75:391–404

    Article  Google Scholar 

  • Farooq M, Basra SMA, Hafeez K, Ahmad N (2005) Thermal hardening: a new seed vigor enhancing tool in rice. J Integr Plant Biol 47:187–193

    Article  Google Scholar 

  • Farooq M, Basra SMA, Hafeez K (2006) Seed invigoration by osmohardening in coarse and fine rice. Seed Sci Technol 34:181–187

    Article  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutase: I. occurrence in higher plants. Plant Physiol 59:309–314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  CAS  PubMed  Google Scholar 

  • Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68:14–25

    Article  CAS  Google Scholar 

  • Hussain S, Khaliq A, Matloob A, Wahid MA, Afzal I (2013) Germination and growth response of three wheat cultivars to NaCl salinity. Soil Environ 32:36–43

    CAS  Google Scholar 

  • Hussain S, Khaliq A, Matloob A, Fahad S, Tanveer A (2015a) Interference and economic threshold level of little seed canary grass in wheat under different sowing times. Environ Sci Pollut Res 22:441–449

    Article  Google Scholar 

  • Hussain S, Zheng M, Khan F, Khaliq A, Fahad S, Peng S, Huang J, Cui K, Nie L (2015b) Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Sci Rep 5:8101. https://doi.org/10.1038/srep08101

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hussain S, Khan F, Cao W, Wu L, Geng M (2016) Seed priming alters the production and detoxification of reactive oxygen intermediates in rice seedlings grown under sub-optimal temperature and nutrient supply. Front Plant Sci 7:439. https://doi.org/10.3389/fpls.2016.00439

    PubMed  PubMed Central  Google Scholar 

  • Iqbal M, Ashraf M (2007) Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants. J Integr Plant Biol 49:1003–1015

    Article  CAS  Google Scholar 

  • Jisha KC, Vijayakumari K, Puthur JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35:1381–1396

    Article  Google Scholar 

  • Khaliq A, Zia-ul-Haq M, Ali F, Aslam F, Matloob A, Navab A, Hussain S (2015) Salinity tolerance in wheat cultivars is related to enhanced activities of enzymatic antioxidants and reduced lipid peroxidation. Clean Soil Air Water 43:1248–1258

    Article  CAS  Google Scholar 

  • Khan HA, Pervez MA, Ayub CM, Ziaf K, Balal RM, Shahid MA, Akhtar N (2009) Hormonal priming alleviates salt stress in hot pepper (Capsicum annuum L.). Soil Environ 28:130–135

    CAS  Google Scholar 

  • Lai K, Berkman PJ, Lorenc MT, Duran C, Smits L, Manoli S et al (2012) Wheat genome.info: an integrated database and portal for wheat genome information. Plant Cell Physiol 53:e2

    Article  CAS  PubMed  Google Scholar 

  • Lee SS, Kim JH (2000) Total sugars, α-amylase activity, and emergence after priming of normal and aged rice seeds. Korean Crop Sci 45:108–111

    Google Scholar 

  • Lichtenthaler HK (1987) Chlorophyll and carotenoids: pigments of photosynthetic bio-membranes. In: Packer L, Douce R (eds) Methods in enzymology. Academic Press, San Diego, pp 350–382

    Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681

    Article  CAS  PubMed  Google Scholar 

  • Munns R, James RA, Lauchli A (2006) Approaches to increasing the salt tolerance of wheat and other cereals. J Exp Bot 57:1025–1043

    Article  CAS  PubMed  Google Scholar 

  • Mustafa Z, Pervez MA, Ayyub CM, Matloob A, Khaliq A, Hussain S, Ihsan MZ, Butt M (2014) Morpho-physiological characterization of chilli genotypes under NaCl salinity. Soil Environ 33:133–141

    Google Scholar 

  • Paparella S, Araújo SS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A (2015) Seed priming: state of the art and new perspectives. Plant Cell Rep. https://doi.org/10.1007/s00299-015-1784-y

    PubMed  Google Scholar 

  • Randhir R, Shetty K (2005) Developmental stimulation of total phenolics and related antioxidant activity in light and dark germinated maize by natural elicitors. Proc Biochem 40:1721–1732

    Article  CAS  Google Scholar 

  • Ruan S, Xue Q, Tylkowska K (2002) The influence of priming on germination of rice (Oryza sativa L.) seeds and seedling emergence and performance in flooded soils. Seed Sci Technol 30:61–67

    Google Scholar 

  • Szalai G, Janda T (2009) Effect of salt stress on the salicylic acid synthesis in young maize (Zea mays L.) plants. J Agron Crop Sci 195:165–171

    Article  CAS  Google Scholar 

  • Tanveer M, Shah AN (2017) An insight into salt stress tolerance mechanisms of Chenopodium album. Environ Sci Pollut Res 24:16531–16535

    Article  CAS  Google Scholar 

  • Zhang S, Hu J, Zhang Y, Xie XJ, Knapp A (2007) Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Aust J Agric Res 58:811–815

    Article  CAS  Google Scholar 

  • Zheng M, Tao Y, Hussain S, Jiang Q, Peng S, Huang J, Cui K, Nie L (2016) Seed priming in dry direct-seeded rice: consequences for emergence, seedling growth and associated metabolic events under drought stress. Plant Growth Regul 78:167–178

    Article  CAS  Google Scholar 

  • Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71

    Article  CAS  PubMed  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Physiol 53:247–273

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Agronomy, University of Agriculture, Faisalabad, Punjab, 38040, Pakistan

    Saddam Hussain & Abdul Khaliq

  2. School of Land and Food, University of Tasmania, Hobart, Australia

    Mohsin Tanveer

  3. Department of Agronomy, Muhammad Nawaz Shareef University of Agriculture, Multan, Punjab, Pakistan

    Amar Matloob

  4. College of Agronomy and Biotechnology, Southwest University, Chongqing, China

    Hafiz Athar Hussain

Authors
  1. Saddam Hussain
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  2. Abdul Khaliq
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  3. Mohsin Tanveer
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  4. Amar Matloob
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  5. Hafiz Athar Hussain
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Corresponding authors

Correspondence to Saddam Hussain or Mohsin Tanveer.

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The authors declare no conflict of interest.

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Communicated by S. Weidner.

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Hussain, S., Khaliq, A., Tanveer, M. et al. Aspirin priming circumvents the salinity-induced effects on wheat emergence and seedling growth by regulating starch metabolism and antioxidant enzyme activities. Acta Physiol Plant 40, 68 (2018). https://doi.org/10.1007/s11738-018-2644-5

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